Jagged 1 Antibody | Rockland Immunochemicals 200-401-698S product information

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product summary

company name :

Rockland Immunochemicals

product type :

antibody

product name :

Jagged 1 Antibody

catalog :

200-401-698S

quantity :

25 µL

price :

99.00 USD

clonality :

polyclonal

host :

rabbit

conjugate :

nonconjugated

reactivity :

human, mouse

application :

western blot, ELISA, immunohistochemistry

product information

Catalog Number :

200-401-698S

Name :

Anti-Jagged-1 (RABBIT) Antibody - 200-401-698S

Display Name :

Jagged 1 Antibody

Application Note :

This protein A purified antibody has been tested for use in ELISA, immunohistochemistry, immunofluorescence microscopy and western blot. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 150 kDa in size corresponding to Jagged-1 in mouse liver whole cell lysates, and a 75 kDa band in human brain and kidney lysates.

Buffer :

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Clonality :

Polyclonal

Concentration Value :

1.1 mg/mL

Concentration Definition :

by UV absorbance at 280 nm

Conjugation :

(None)

Size :

25 µL

Default Unit :

ELISA Dilution :

1:20,000 - 1:100,000

Immunohistochemistry Dilution :

1:100 - 1:500

IF Microscopy Dilution :

1:200 - 1:1,000

Western Blot Dilution :

1:500 - 1:2,000

Expiration :

Expiration date is one (1) year from date of opening.

Format :

IgG

Gene Name :

JAG1

Host Animal :

Rabbit

General Disclaimer Note :

This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.

Immunogen :

This protein A purified Jagged 1 antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to amino acids near the N-terminus of human Jagged-1 protein.

Packing Type :

Dry Ice

Physical State :

Liquid (sterile filtered)

Preservative :

0.01% (w/v) Sodium Azide

Purity and Specificity :

This protein A purified antibody is directed against human Jagged-1 protein. The product was purified from mono-specific antiserum by affinity chromatography. A BLAST analysis was used to suggest cross reactivity with Jagged-1 protein from human, chimpanzee, rat and mouse based on 100% homology with the immunizing sequence. Partial reactivity is expected against dog (81%) and Xenopus laevis (85%) based on partial sequence homologies as indicated. Reactivity against homologues from other sources is not known.

Species Reactivity :

Human, mouse

Storage :

Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 µL). To minimize loss of volume dilute 1:10 by adding 225 µL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.

Synonyms :

rabbit anti-Jagged 1 Antibody, rabbit anti-Jagged1 Antibody, rabbit anti-Jagged-1 Antibody, Ser 1 antibody, AGS antibody, AHD antibody, AWS antibody, CD 339 antibody, CD339 antibody, CD339 antigen antibody, Headturner antibody, HJ1 antibody, Htu antibody

Background :

Anti-Jagged 1 Antibody recognizes the Jagged 1 protein encoded by the JAG1 gene, that is the human homolog of the Drosophilia jagged protein. Human Jagged 1 is the ligand for multiple Notch receptors and mediation of Notch signaling. Jagged 1 signaling through Notch 1 has also been shown to play a role in hematopoiesis. Mutations that alter the Jagged 1 protein cause Alagille syndrome, deafness, congenital heart defects, and posterior embryotoxon. Anti-Jagged-1 Antibody is useful for researchers interested in Notch activation, Growth Factor activities, and Cardiovascular health.

General Reference :

Yabe,Y., Matsumoto,T., Tsurumoto,T. and Shindo,H. (2005) Immunohistological localization of Notch receptors and their ligands Delta and Jagged in synovial tissues of rheumatoid arthritis. J Orthop Sci 10 (6), 589-594. Veeraraghavalu,K., Subbaiah,V.K., Srivastava,S., Chakrabarti,O., Syal,R. and Krishna,S. (2005) Complementation of human papillomavirus type 16 E6 and E7 by Jagged1-specific Notch1-phosphatidylinositol 3-kinase signaling involves pleiotropic oncogenic functions independent of CBF1;Su(H);Lag-1 activation. J. Virol. 79 (12), 7889-7898. Kuure,S., Sainio,K., Vuolteenaho,R., Ilves,M., Wartiovaara,K., Immonen,T., Kvist,J., Vainio,S. and Sariola,H. (2005) Crosstalk between Jagged1 and GDNF/Ret/GFRalpha1 signalling regulates ureteric budding and branching. Mech. Dev. 122 (6), 765-780.

Immunogen Type :

Peptide

Low Endotoxin :

Sample Size :

Yes

Target Domain of Reactivity :

Internal

Application Text :

ELISA,Immunohistochemistry,IF Microscopy,Western Blot,

Other :

User Optimized

Category :

Primary Antibodies

Conjugation Name :

Unconjugated

2019 Price :

99.00 USD

UniProt :

P78504

NCBI :

4557679

Primary Image Name :

Immunofluorescence - Jagged-1 Antibody