NOTCH 1 Antibody | Rockland Immunochemicals 100-401-407S product information

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product summary

company name :

Rockland Immunochemicals

product type :

antibody

product name :

NOTCH 1 Antibody

catalog :

100-401-407S

quantity :

25 µL

price :

99.00 USD

clonality :

polyclonal

host :

rabbit

conjugate :

nonconjugated

reactivity :

human

application :

western blot, ELISA, immunohistochemistry

product information

Catalog Number :

100-401-407S

Name :

Anti-NOTCH 1 (Cleaved N terminal) (Human specific) (RABBIT) Antibody - 100-401-407S

Display Name :

NOTCH 1 Antibody

Application Note :

This antibody is reactive with Notch 1 by western blotting, Immunohistochemistry (IHC) and ELISA. An 80 kDa band corresponding to Notch 1 was observed at a 1:500 dilution. Specific conditions for reactivity should be optimized by the end user.

Buffer :

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Clonality :

Polyclonal

Concentration Value :

80 mg/mL

Concentration Definition :

by Refractometry

Conjugation :

(None)

Size :

25 µL

Default Unit :

ELISA Dilution :

1:20,000 - 1:60,000

Immunohistochemistry Dilution :

1:200

Western Blot Dilution :

1:500- 1:2,000

Expiration :

Expiration date is one (1) year from date of opening.

Format :

Antiserum

Gene Name :

NOTCH1

Host Animal :

Rabbit

General Disclaimer Note :

This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.

Immunogen :

This whole rabbit serum was prepared by repeated immunizations with a synthetic peptide corresponding to amino acid residues of human Notch 1 located near the N-terminal sequence of the cleaved N intracellular domain (NICD).

Packing Type :

Dry Ice

Physical State :

Liquid (sterile filtered)

Preservative :

0.1% (w/v) Sodium Azide

Purity and Specificity :

This antiserum is directed against human NOTCH 1. Based on the immunogen sequence, we expect this antibody to react as well with mouse and rat NOTCH 1 (100% sequence homology). This antibody reacts with mouse Notch constructs present in lysates of HEK 293 cells. Only the cleaved intracellular (activated) form (NICD) is detected. No reactivity is detected against mouse N2, N3 or N4. The immunogen epitope is only exposed after gamma secretase cleavage and is not accessible in the uncleaved form.

Species Reactivity :

human, mouse, rat

Storage :

Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 µL). To minimize loss of volume dilute 1:10 by adding 225 µL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.

Synonyms :

rabbit anti-notch1 antibody, Neurogenic locus Notch homolog protein 1, hN1, Translocation-associated Notch protein TAN-1

Target Species :

Human

Background :

Anti Notch 1 Antibody recognizes Notch 1 that is synthesized in the endoplasmic reticulum as an inactive form which is proteolytically cleaved by a furin-like convertase (S1 cleavage) in the trans-golgi network before it reaches the plasma membrane to yield an active, ligand-accessible form. Cleavage results in a C-terminal fragment N(TM) and a N-terminal fragment N(EC). Following ligand binding, it is cleaved (S2 cleavage) by TNF-alpha converting enzyme (TACE) to yield a membrane-associated intermediate fragment called Notch extracellular truncation (NEXT). This fragment is then cleaved by presenilin-dependent gamma-secretase (S3 cleavage) to release the intracellular domain (NICD) from the membrane.

General Reference :

Bertrand FE, Eckfeldt CE, Lysholm AS, LeBien TW (2000) Notch-1 and Notch-2 exhibit unique patterns of expression in human B-lineage cells. Leukemia 12: 2095-2102. Gridley T (2003) Notch signaling and inherited disease syndromes. Hum. Mol. Genet. 12: Suppl 1, R9-R13.

Specific Reference :

Gupta S, Li S, Abedin Md J, Wang L, Schneider E, Najafian B, Rosenberg M. Effect of Notch activation on the regenerative response to acute renal failure. Am J Physiol Renal Physiol 298: F209–F215, 2010. Published online before print October 14, 2009, doi: 10.1152/ajprenal.00451.200 Solomon A, Mian Y, Ortega-Cava C, et al. Upregulation of the let-7 microRNA with precocious development in lin-12/Notch hypermorphic Caenorhabditis elegans mutants. Dev. Biol. 2008 Apr 15;316(2):191–199. Stylianou S, Clarke RB, Brennan K. Aberrant activation of notch signaling in human breast cancer. Cancer Res. 2006 Feb 1;66(3):1517–1525. E Poli, C Zalfa, F D’Avanzo, R Tomanin, L Carlessi, M Bossi, L Rota Nodari, E Binda, P Marmiroli, M Scarpa, D Delia, A L Vescovi, L De Filippis. (2013) Murine neural stem cells model Hunter disease in vitro: glial cell-mediated neurodegeneration as a possible mechanism involved. Cell Death & Disease vol4, page e906.

Immunogen Type :

Peptide

Low Endotoxin :

Sample Size :

Yes

Target Domain of Reactivity :

N-Terminus

Application Text :

ELISA,Immunohistochemistry,Western Blot,

Other :

User Optimized

Category :

Primary Antibodies

Conjugation Name :

Unconjugated

2019 Price :

99.00 USD

UniProt :

P46531

Primary Image Name :

Immunohistochemistry- anti-Notch1 antibody