Osteopontin Antibody | Rockland Immunochemicals 100-401-404S product information

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product summary

company name :

Rockland Immunochemicals

product type :

antibody

product name :

Osteopontin Antibody

catalog :

100-401-404S

quantity :

25 µL

price :

99.00 USD

clonality :

polyclonal

host :

rabbit

conjugate :

nonconjugated

reactivity :

human

application :

western blot, ELISA, immunohistochemistry, immunoprecipitation

product information

Catalog Number :

100-401-404S

Name :

Anti-Osteopontin (RABBIT) Antibody - 100-401-404S

Display Name :

Osteopontin Antibody

Application Note :

Anti-Osteopontin is suitable for western blotting, immunohistochemistry (formalin-fixed paraffin-embedded sections) and ELISA. The antibody exclusively recognizes C-terminal fragments of both thrombin and MMP-cleaved OPN. A 1:1000 dilution will detect strongly approximately 250 ng of OPN protein on a blot. No pretreatment is required for IHC when formalin-fixed paraffin-embedded tissue is stained.

Buffer :

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Clonality :

Polyclonal

Concentration Value :

75 mg/mL

Concentration Definition :

by Refractometry

Conjugation :

(None)

Size :

25 µL

Default Unit :

ELISA Dilution :

1:5,000 - 1:20,000

Immunohistochemistry Dilution :

1:100 - 1:300

Immunoprecipitation Dilution :

1:100

Western Blot Dilution :

1:500 - 1:2,000

Expiration :

Expiration date is one (1) year from date of opening.

Format :

Antiserum

Gene Name :

SPP1, BNSP, OPN

Host Animal :

Rabbit

General Disclaimer Note :

This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.

Immunogen :

This whole rabbit serum was prepared by repeated immunizations with a synthetic peptide, from the human osteopontin protein, conjugated to KLH using maleimide.

Packing Type :

Dry Ice

Physical State :

Liquid (sterile filtered)

Preservative :

0.1% (w/v) Sodium Azide

Purity and Specificity :

Osteopontin is directed against human osteopontin. The antibody recognizes the full-length osteopontin protein (which runs at 66 kDa on westerns), as well as the C-terminal fragments of both thrombin and MMP-cleaved OPN. The 32 kDa MMP-cleaved C-fragment is recognized, but not the 40 kDa N-terminal fragment. Reactivity is reported to occur with osteopontin from swine, dog, mouse and rat.

Species Reactivity :

human,mouse, rat, dog, swine

Storage :

Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 µL). To minimize loss of volume dilute 1:10 by adding 225 µL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.

Synonyms :

rabbit anti-Osteopontin antibody, Bone sialoprotein 1, Secreted phosphoprotein 1, SPP-1, Urinary stone protein, Nephropontin, Uropontin, SPP1, BNSP, OPN

Target Species :

Human

Background :

Anti Osteopontin Antibody recognizes Osteopontin (OPN) that is an arginine-glycine-aspartic acid (RGD)-containing glycoprotein which interacts with integrins and CD44 as major receptors. OPN is multifunctional, with activities in cell migration, cell survival, inhibition of calcification, regulation of immune cell function, and control of tumor cell phenotype. The gene encoding OPN is called spp1. Targeting this gene has revealed that while OPN is not necessary for normal embryonic development, fertility and health under pathogen-free conditions, loss of the protein has significant consequences in several models of injury/disease as diverse as renal injury, viral and bacterial infection, bone remodeling, and tumor growth. The fact that no other proteins seem to share a redundant activity with OPN under these conditions suggests that OPN has a unique functional role during tissue injury and stress. Interestingly, several members of the matrix metalloproteinase (MMP) family are also induced during injury/disease processes in patterns overlapping that of OPN. OPN has recently been shown to be a novel substrate for two MMPs, MMP-3 (stromelysin-1) and MMP-7 (matrilysin). There are three cleavage sites for MMP-3 in human OPN, two of which are also cleaved by MMP-7 (see cleavage diagram). Biological assays demonstrate that the MMP-cleaved OPN has increased activity in promoting both cell adhesion and migration compared with full-length OPN. In addition, inhibitory reagents were used to show that the same receptors that interact with OPN also mediate interaction of MMP-cleaved OPN with tumor cells. It is suggested that active forms of OPN at sites of tissue injury may be regulated by the activity of proteases including MMPs and that the differences in activity of modified OPN may be explained by differences in binding affinity of integrins or distinct downstream signaling events.

General Reference :

Weber, G. F. and Cantor, H. (1996) Cytokine Growth Factor Rev. 7, 241–248. Denhardt, D. T., Lopez, C. A., Rollo, E. E., Hwang, S. M., An, X. R. and Walther, S. E. (1995) Ann. N. Y. Acad. Sci. 760, 127–142. Denhardt, D. T. and Chambers, A. F. (1994) J. Cell. Biochem. 56, 48–51. Uede, T., Katagiri, Y., Iizuka, J. and Murakami, M. (1997) Microbiol. Immunol. 41, 641–648.

Specific Reference :

Kim SJ, Shin TK. Immunohistochemical study of osteopontin in boar testis. J Vet Sci. 2007 June; 8(2): 107–110. Published online 2007 June 30. doi: 10.4142/jvs.2007.8.2.107 PMCID: PMC2872706 Robertson BW, Bonsal L, Chellaiah MA. Regulation of Erk1/2 activation by osteopontin in PC3 human prostate cancer cells. Mol. Cancer. 2010;9:260. Robertson BW, Chellaiah MA. Osteopontin induces beta-catenin signaling through activation of Akt in prostate cancer cells. Exp. Cell Res. 2010 Jan 1;316(1):1–11. Cherepanova O, Gomez D, Shankman L, Swiatlowska P, Williams J, Sarmento O, Alencar G, Hess D, Bevard M, Greene E, Murgai M, Turner S, Geng Y, Bekiranov S, Connelly J, Tomilin A, Owens G. (2016) Activation of the pluripotency factor OCT4 in smooth muscle cells is atheroprotective. Nature Medicine volume 22, pages 657–665.

Immunogen Type :

Peptide

Low Endotoxin :

Sample Size :

Yes

Target Domain of Reactivity :

Internal

Application Text :

ELISA,Immunohistochemistry,Immunoprecipitation,Western Blot,

Other :

User Optimized

Category :

Primary Antibodies

Conjugation Name :

Unconjugated

2019 Price :

99.00 USD

UniProt :

P10451

Primary Image Name :

Anti-Osteopontin Anitbody - Immunohistochemistry