AKT Antibody | Rockland Immunochemicals 100-401-401S product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Rockland Immunochemicals

product type :

antibody

product name :

AKT Antibody

catalog :

100-401-401S

quantity :

25 µL

price :

99.00 USD

clonality :

polyclonal

host :

rabbit

conjugate :

nonconjugated

reactivity :

human, mouse, rat, chicken

application :

western blot, ELISA, immunohistochemistry, flow cytometry

product information

Catalog Number :

100-401-401S

Name :

Anti-AKT (RABBIT) Antibody - 100-401-401S

Display Name :

AKT Antibody

Application Note :

AKT Antibody is suitable for western blotting to detect a single band of the expected apparent molecular weight, immunohistochemistry (Formalin-fixed paraffin-embedded sections), immunofluorescence microscopy using paraformaldehyde-fixed primary cardiomyocyte cultures, immunoprecipitation, transfected cell culture, primary cell culture and ELISA. Researchers should determine optimal titers for applications that are not stated below.

Buffer :

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Clonality :

Polyclonal

Concentration Value :

75 mg/ml

Concentration Definition :

by Refractometry

Conjugation :

(None)

Size :

25 µL

Default Unit :

ELISA Dilution :

1:2,000 - 1:10,000

Flow Cytometry Dilution :

User Optimized

Immunohistochemistry Dilution :

1:500 - 1:2,000

IF Microscopy Dilution :

1:100 - 1:1,000

Western Blot Dilution :

1:500 - 1:2,000

Format :

Antiserum

Gene Name :

AKT1

Host Animal :

Rabbit

General Disclaimer Note :

This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.

Immunogen :

AKT Antibody was produced from whole rabbit serum prepared by repeated immunizations with a synthetic peptide R-P-H-F-P-Q-F-S-Y-S-A-S-G-T-A corresponding to the C-terminus (460-480) of human AKT proteins conjugated to KLH using maleimide. A residue of cysteine was added to the amino terminal end to facilitate coupling. A BLAST analysis was used to suggest reactivity with this protein from rat, mouse, and chicken based on 100% homology for the immunogen sequence.

Packing Type :

Dry Ice

Physical State :

Liquid (sterile filtered)

Preservative :

0.1% (w/v) Sodium Azide

Purity and Specificity :

This product was prepared from monospecific antiserum by a delipidation and defibrination. Pan Anti-AKT Antibody reacts with the AKT from human tissues. Based on sequence we expect this antibody to react as well with rat, mouse, and chicken AKT.

Species Reactivity :

human, mouse, rat, chicken

Storage :

Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 µL). To minimize loss of volume dilute 1:10 by adding 225 µL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.

Synonyms :

rabbit anti-AKT antibody, RAC-PK-alpha, Protein kinase B, PKB, C-AKT, RAC-alpha serine/threonine-protein kinase, Proto-oncogene c-Akt, AKT1, AKT 1, AKT-1

Target Species :

Human

Background :

AKT Antibody detects AKT which is a component of the PI-3 kinase pathway and is activated by phosphorylation at Ser 473 and Thr 308. AKT is a cytoplasmic protein also known as Protein Kinase B (PKB) and rac (related to A and C kinases). AKT is a key regulator of many signal transduction pathways. AKT Exhibits tight control over cell proliferation and cell viability. Overexpression or inappropriate activation of AKT is noted in many types of cancer. AKT mediates many of the downstream events of PI 3-kinase (a lipid kinase activated by growth factors, cytokines and insulin). PI 3-kinase recruits AKT to the membrane, where it is activated by PDK1 phosphorylation. Once phosphorylated, AKT dissociates from the membrane and phosphorylates targets in the cytoplasm and the cell nucleus. AKT has two main roles: (i) inhibition of apoptosis; (ii) promotion of proliferation. Anti-AKT Antibody is ideal for investigators involved in Cell Signaling, Neuroscience, Signal Transduction, VEGF Signaling, and apoptosis research.

General Reference :

Lawlor, M. A. and Alessi, D.R. (2001). PKB/AKT: a key mediator of cell proliferation, survival and insulin responses. J. Cell Science 114:2903-2910. Alessi, D. R. (2001). Discovery of PDK1, one of the missing links in insulin signal transduction. Biochem. Soc. Trans. 29,1 -14.

Specific Reference :

Matsuda N, Takano Y, Kageyama S-I, et al. Silencing of caspase-8 and caspase-3 by RNA interference prevents vascular endothelial cell injury in mice with endotoxic shock. Cardiovasc. Res. 2007 Oct 1;76(1):132-140. Takeuchi R, Ryo A, Komitsu N, et al. Low-intensity pulsed ultrasound activates the phosphatidylinositol 3 kinase/Akt pathway and stimulates the growth of chondrocytes in three-dimensional cultures: a basic science study. Arthritis Res Ther. 2008;10(4):R77

Immunogen Type :

Peptide

Low Endotoxin :

Sample Size :

Yes

Target Domain of Reactivity :

C-Terminus

Application Text :

ELISA,Flow Cytometry,Immunohistochemistry,IF Microscopy,Western Blot,

Other :

User Optimized

Category :

Primary Antibodies

Conjugation Name :

Unconjugated

2019 Price :

99.00 USD

UniProt :

P31749

NCBI :

62241011

Primary Image Name :

Anti-AKT Antibody - Immunofluorescence Microscopy