BDNF Human Recombinant Protein | Rockland Immunochemicals 009-001-C27 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Rockland Immunochemicals

product type :

protein

product name :

BDNF Human Recombinant Protein

catalog :

009-001-C27

quantity :

10 µg

price :

244.00 USD

product information

Catalog Number :

009-001-C27

Name :

BDNF Human Recombinant Protein - 009-001-C27

Display Name :

BDNF Human Recombinant Protein

Application Note :

BDNF is suitable as a control for polyclonal or monoclonal anti-BDNF in immunological assays. Biological Activity: The activity is determined by the dose-dependent proliferation of C6 cells and is typically between 0.5-1.5 µg/mL.

Concentration Value :

0.1 mg/mL

Concentration Definition :

by UV absorbance at 280 nm

Conjugation :

(None)

Size :

10 µg

Default Unit :

µg

Expiration :

Expiration date is six (6) months from date of opening.

Gene Name :

BDNF

Host Animal :

Other - E.coli

General Disclaimer Note :

This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.

Other Performance Data :

Biological Activity: BDNF activity is determined by the dose-dependent proliferation of C6 cells and is typically between 0.5-1.5 μg/mL. Endotoxin Level: Measured by kinetic LAL analysis and is typically ≤ 1 EU/μg protein.

Packing Type :

Ambient

Physical State :

Lyophilized

Purity and Specificity :

Purity was determined to be greater than 97% as determined by analysis by RP-HPLC and by reducing and non-reducing SDS-PAGE against a known standard. Endotoxin level as determined by kinetic LAL analysis: 0.05 EUs/µg protein.

Reconstitution Buffer :

Restore with deionized water (or equivalent)

Reconstitution Volume :

10µl (10-100µl)

Species of Origin :

Human

Species Reactivity :

Human

Storage :

Store vial at 4° C prior to restoration. Dilute only prior to immediate use. Maintain sterility. This product DOES NOT contain preservative. DO NOT VORTEX. We recommend adding a carrier protein such as HSA or BSA to 0.1% (i.e. 1.0 mg/mL) . For best results aliquot contents and freeze at -20° C or colder. Avoid cycles of freezing and thawing. Centrifuge vial before each opening to dislodge contents from the cap and to clarify if contents are not clear after standing at room temperature.

Synonyms :

Brain-derived neurotrophic factor, Abrineurin cytokine

Background :

BDNF is a member of the nerve growth factor family of trophic factors. In the brain BDNF has a trophic action on retinal, cholinergic, and dopaminergic neurons, and in the peripheral nervous system it acts on both motor and sensory neurons. Some protein domains of BDNF are identical with those of NGF and another neurotrophic factor, designated NT-3 (neurotrophin-3). It exists as monomers and homodimers, and binds to NTRK2/TRKB. Polyclonal antibodies raised against murine NGF have been shown to cross-react with both NT-3 and BDNF. The propeptide is N-glycosylated and glycosulfated. BDNF is converted into mature BDNF by plasmin (PLG). It is expressed in brain, and highly expressed in hippocampus, amygdala, cerebral cortex and cerebellum; it is also expressed in heart, lung, skeletal muscle, testis, prostate and placenta. Recombinant Human Brain-Derived Neurotrophic Factor produced in E.coli is a homodimer, non-glycosylated, polypeptide chain containing 2 x 119 amino acids and having a total molecular mass of 26,984 Daltons.

General Reference :

Liu Q.-R., Walther D., Drgon T., Polesskaya O., Lesnick T.G., Strain K.J., de Andrade M., Bower J.H., Maraganore D.M., Uhl G.R. (2005) Human brain derived neurotrophic factor (BDNF) genes, splicing patterns, and assessments of associations with substance abuse and Parkinson's Disease. Am. J. Med. Genet. B Neuropsychiatr. Genet. 134:93-103(2005) [PubMed: 15666411] [Abstract] Rosenfeld R.D., Zeni L., Haniu M., Talvenheimo J., Radka S.F., Bennett L., Miller J.A., Welcher A.A. (1995) Purification and identification of brain-derived neurotrophic factor from human serum. Protein Expr. Purif. 6:465-471(1995) [PubMed: 8527932] [Abstract]

Low Endotoxin :

Yes

PSC Format :

Recombinant Protein

PSC Type :

Cytokine, Interleukin or Growth Factor

Sample Size :

Yes

Other :

User Optimized

Category :

Proteins and Peptides

Conjugation Name :

Unconjugated

2019 Price :

244.00 USD

UniProt :

P23560

NCBI :

3YAWJEK2011

Primary Image Name :

BDNF Human Recombinant Protein - SDS-PAGE