product summary
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company name :
R&D Systems
product type :
antibody
product name :
Human/Mouse Active Caspase-3 Antibody
catalog :
AF835
quantity :
50 ug (also 25 ug)
price :
389 USD
clonality :
polyclonal
host :
domestic rabbit
conjugate :
nonconjugated
reactivity :
human, mouse, rat, dogs, chicken
application :
western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation, flow cytometry, immunohistochemistry - paraffin section, immunohistochemistry - frozen section
more info or order :
citations: 194
Published Application/Species/Sample/DilutionReference
  • immunohistochemistry - paraffin section; mouse; 1:1000; loading ...; fig s1f
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  • immunohistochemistry - paraffin section; mouse; loading ...; fig 7c
Simpson D, Pang J, Weir A, Kong I, Fritsch M, Rashidi M, et al. Interferon-γ primes macrophages for pathogen ligand-induced killing via a caspase-8 and mitochondrial cell death pathway. Immunity. 2022;55:423-441.e9 pubmed publisher
  • immunohistochemistry - paraffin section; mouse; loading ...; fig 1d
Lee C, Kim J, Han J, Oh D, Kim M, Jeong H, et al. Formyl peptide receptor 2 determines sex-specific differences in the progression of nonalcoholic fatty liver disease and steatohepatitis. Nat Commun. 2022;13:578 pubmed publisher
  • immunohistochemistry - paraffin section; mouse; 1 ug/ml; loading ...; fig s1c
Williams H, Wadey K, Frankow A, Blythe H, Forbes T, Johnson J, et al. Aneurysm severity is suppressed by deletion of CCN4. J Cell Commun Signal. 2021;15:421-432 pubmed publisher
  • immunohistochemistry - frozen section; mouse; loading ...; fig s1e
Kuijpers M, Kochlamazashvili G, Stumpf A, Puchkov D, Swaminathan A, Lucht M, et al. Neuronal Autophagy Regulates Presynaptic Neurotransmission by Controlling the Axonal Endoplasmic Reticulum. Neuron. 2021;109:299-313.e9 pubmed publisher
Duncan G, Ingram S, Emberley K, Hill J, Cordano C, Abdelhak A, et al. Remyelination protects neurons from DLK-mediated neurodegeneration. Nat Commun. 2024;15:9148 pubmed publisher
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Brockmueller A, Girisa S, Kunnumakkara A, Shakibaei M. Resveratrol Modulates Chemosensitisation to 5-FU via β1-Integrin/HIF-1α Axis in CRC Tumor Microenvironment. Int J Mol Sci. 2023;24: pubmed publisher
Provitera L, Tomaselli A, Raffaeli G, Crippa S, Arribas C, Amodeo I, et al. Human Bone Marrow-Derived Mesenchymal Stromal Cells Reduce the Severity of Experimental Necrotizing Enterocolitis in a Concentration-Dependent Manner. Cells. 2023;12: pubmed publisher
Elrod J, Lenz M, Kiwit A, Armbrust L, Sch xf6 nfeld L, Reinshagen K, et al. Murine scald models characterize the role of neutrophils and neutrophil extracellular traps in severe burns. Front Immunol. 2023;14:1113948 pubmed publisher
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Van Heurck R, Bonnefont J, Wojno M, Suzuki I, Velez Bravo F, Erkol E, et al. CROCCP2 acts as a human-specific modifier of cilia dynamics and mTOR signaling to promote expansion of cortical progenitors. Neuron. 2023;111:65-80.e6 pubmed publisher
Piekna Przybylska D, Na D, Zhang J, Baker C, Ashton J, White P. Single cell RNA sequencing analysis of mouse cochlear supporting cell transcriptomes with activated ERBB2 receptor indicates a cell-specific response that promotes CD44 activation. Front Cell Neurosci. 2022;16:1096872 pubmed publisher
Platt M, Lin Y, Penix T, Wiscovitch Russo R, Vashee I, Mares C, et al. A multiomics analysis of direct interkingdom dynamics between influenza A virus and Streptococcus pneumoniae uncovers host-independent changes to bacterial virulence fitness. PLoS Pathog. 2022;18:e1011020 pubmed publisher
Ortiz xc1 lvarez G, Fortoul A, Srivastava A, Moreau M, Bouloudi B, Mailhes Hamon C, et al. p53/p21 pathway activation contributes to the ependymal fate decision downstream of GemC1. Cell Rep. 2022;41:111810 pubmed publisher
Piacentino M, Hutchins E, Andrews C, Bronner M. Temporal changes in plasma membrane lipid content induce endocytosis to regulate developmental epithelial-to-mesenchymal transition. Proc Natl Acad Sci U S A. 2022;119:e2212879119 pubmed publisher
Karshieva S, Glinskaya E, Dalina A, Akhlyustina E, Makarova E, Khesuani Y, et al. Antitumor activity of photodynamic therapy with tetracationic derivative of synthetic bacteriochlorin in spheroid culture of liver and colon cancer cells. Photodiagnosis Photodyn Ther. 2022;40:103202 pubmed publisher
Brockmueller A, Mueller A, Shayan P, Shakibaei M. β1-Integrin plays a major role in resveratrol-mediated anti-invasion effects in the CRC microenvironment. Front Pharmacol. 2022;13:978625 pubmed publisher
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Brockmueller A, Shayan P, Shakibaei M. Evidence That β1-Integrin Is Required for the Anti-Viability and Anti-Proliferative Effect of Resveratrol in CRC Cells. Int J Mol Sci. 2022;23: pubmed publisher
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Dong X, Yang H, Zhou X, Xie X, Yu D, Guo L, et al. LIM-Homeodomain Transcription Factor LHX4 Is Required for the Differentiation of Retinal Rod Bipolar Cells and OFF-Cone Bipolar Subtypes. Cell Rep. 2020;32:108144 pubmed publisher
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Sarkar T, Patro N, Patro I. Neuronal changes and cognitive deficits in a multi-hit rat model following cumulative impact of early life stressors. Biol Open. 2020;9: pubmed publisher
Xi L, Carroll T, Matos I, Luo J, Polak L, Pasolli H, et al. m6A RNA methylation impacts fate choices during skin morphogenesis. elife. 2020;9: pubmed publisher
Nokin M, Darbo E, Travert C, Drogat B, Lacouture A, San José S, et al. Inhibition of DDR1 enhances in vivo chemosensitivity in KRAS-mutant lung adenocarcinoma. JCI Insight. 2020;5: pubmed publisher
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Auzmendi Iriarte J, Sáenz Antoñanzas A, Mikelez Alonso I, Carrasco Garcia E, Tellaetxe Abete M, Lawrie C, et al. Characterization of a new small-molecule inhibitor of HDAC6 in glioblastoma. Cell Death Dis. 2020;11:417 pubmed publisher
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product information
brand :
R&D Systems
master code :
AF835
SKU :
AF835
product name :
Human/Mouse Active Caspase-3 Antibody
unit size :
50 ug (also 25 ug)
seo description :
The Human/Mouse Active Caspase-3 Antibody from R&D Systems is a rabbit polyclonal antibody to Caspase-3. This antibody reacts with avian - chicken,canine,chicken,equine,feline,fish,fish - cyprinus carpio (carp),fish - oncorhynchus mykiss (rainbow trout),guinea pig,human,mouse,ovine,porcine,primate,rat,transgenic mouse,fish - danio rerio (zebrafish). The Human/Mouse Active Caspase-3 Antibody has been validated for the following applications: Immunohistochemistry,Western Blot,Flow Cytometry,Immunoprecipitation,Functional Assay,Immunohistochemistry-Paraffin,Immunohistochemistry-Frozen,Immunofluorescence,Immunocytochemistry,Immunocytochemistry/ Immunofluorescence.
target :
Caspase-3
category :
Primary Antibodies
buffer :
Lyophilized from a 0.2 ╡m filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 ╡m filtered solution in PBS.
clonality :
Polyclonal
concentration :
LYOPH
conjugate :
Unconjugated
dilution :
Immunohistochemistry 1-15 ug/mL, Immunocytochemistry 5-15 ug/mL
host :
Rabbit
immunogen :
KLH-coupled human Caspase-3 synthetic peptide, CRGTELDCGIETD, Accession # P42574
isotype :
IgG
purity :
Antigen Affinity-purified
species :
Avian - Chicken,Canine,Chicken,Equine,Feline,Fish,Fish - Cyprinus carpio (Carp),Fish - Oncorhynchus mykiss (Rainbow Trout),Guinea Pig,Human,Mouse,Ovine,Porcine,Primate,Rat,Transgenic Mouse,Fish - Danio rerio (Zebrafish)
specificity :
Distinguishes apoptotic cells from non-apoptotic cells in immunohistochemistry. Detects the p17 subunit of Caspase-3, but does not detect the precursor form in Western blot. We recommend R D Systems Affinity Purified Anti-Caspase-3 (Catalog # AF-605-NA) or Monoclonal Anti-Cleaved-Caspase-3 (Catalog # MAB835) for Western blot applications.
gene symbol :
CASP3
top caption :
Caspase-3 antibody in Jurkat Human Cell Line by Immunocytochemistry (ICC).
accessionNumbers :
P42574
applications :
Flow Cytometry,Immunohistochemistry,Immunoprecipitation,Western Blot,Immunohistochemistry-Paraffin,Immunohistochemistry-Frozen,Immunofluorescence,Immunocytochemistry,Functional Assay,Immunocytochemistry/ Immunofluorescence
USD :
389 USD
alt names :
Apopain, apoptosis-related cysteine protease, CASP3, CASP-3, caspase 3, apoptosis-related cysteine peptidase, caspase-3, Caspase3, CPP32, CPP-32, CPP32B, CPP32SREBP cleavage activity 1, Cysteine protease CPP32, EC 3.4.22, EC 3.4.22.56, LICE-1, PARP cleavage protease, procaspase3, Protein Yama, SCA-1, Yama
storage :
Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 ░C as supplied. 1 month, 2 to 8 ░C under sterile conditions after reconstitution. 6 months, -20 to -70 ░C under sterile conditions after reconstitution.
more info or order :
company information
R&D Systems
614 McKinley Place N.E.
Minneapolis, MN 55413
info@RnDSystems.com
https://www.rndsystems.com
800 343-7475
headquarters: USA
R&D Systems develops and manufactures high-quality proteins and serves as a world leader in immunoassays. R&D Systems also produces quality antibodies, antibody arrays, stem cell and cell culture products, and cell selection and detection products, serving the life science and diagnostics industry.