This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
R&D Systems
product type :
protein
product name :
BDNF Protein
catalog :
248-BD-005/CF
quantity :
5 ug (also 250 ug, 10 ug, 25 ug)
price :
229 USD
citations: 57
Reference
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Hartman W, Helan M, Smelter D, Sathish V, Thompson M, Pabelick C, et al. Role of Hypoxia-Induced Brain Derived Neurotrophic Factor in Human Pulmonary Artery Smooth Muscle. PLoS ONE. 2015;10:e0129489 pubmed publisher
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Yoshida M, Kitaoka S, Egawa N, Yamane M, Ikeda R, Tsukita K, et al. Modeling the early phenotype at the neuromuscular junction of spinal muscular atrophy using patient-derived iPSCs. Stem Cell Reports. 2015;4:561-8 pubmed publisher
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Devlin A, Burr K, Borooah S, Foster J, Cleary E, Geti I, et al. Human iPSC-derived motoneurons harbouring TARDBP or C9ORF72 ALS mutations are dysfunctional despite maintaining viability. Nat Commun. 2015;6:5999 pubmed publisher
Bayin N, Modrek A, Dietrich A, Lebowitz J, Abel T, Song H, et al. Selective lentiviral gene delivery to CD133-expressing human glioblastoma stem cells. PLoS ONE. 2014;9:e116114 pubmed publisher
Wainger B, Buttermore E, Oliveira J, Mellin C, Lee S, Saber W, et al. Modeling pain in vitro using nociceptor neurons reprogrammed from fibroblasts. Nat Neurosci. 2015;18:17-24 pubmed publisher
Czarnecka M, Trinh E, Lu C, Kuan Celarier A, Galli S, Hong S, et al. Neuropeptide Y receptor Y5 as an inducible pro-survival factor in neuroblastoma: implications for tumor chemoresistance. Oncogene. 2015;34:3131-43 pubmed publisher
Prè D, Nestor M, Sproul A, Jacob S, Koppensteiner P, Chinchalongporn V, et al. A time course analysis of the electrophysiological properties of neurons differentiated from human induced pluripotent stem cells (iPSCs). PLoS ONE. 2014;9:e103418 pubmed publisher
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Terenzio M, Golding M, Russell M, Wicher K, Rosewell I, Spencer Dene B, et al. Bicaudal-D1 regulates the intracellular sorting and signalling of neurotrophin receptors. EMBO J. 2014;33:1582-98 pubmed publisher
Otis J, Fitzgerald M, Mueller D. Infralimbic BDNF/TrkB enhancement of GluN2B currents facilitates extinction of a cocaine-conditioned place preference. J Neurosci. 2014;34:6057-64 pubmed publisher
Lundgaard I, Luzhynskaya A, Stockley J, Wang Z, Evans K, Swire M, et al. Neuregulin and BDNF induce a switch to NMDA receptor-dependent myelination by oligodendrocytes. PLoS Biol. 2013;11:e1001743 pubmed publisher
Ni N, Hu Y, Ren H, Luo C, Li P, Wan J, et al. Self-assembling peptide nanofiber scaffolds enhance dopaminergic differentiation of mouse pluripotent stem cells in 3-dimensional culture. PLoS ONE. 2013;8:e84504 pubmed publisher
Zhong C, Talmage D, Role L. Nicotine elicits prolonged calcium signaling along ventral hippocampal axons. PLoS ONE. 2013;8:e82719 pubmed publisher
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Osborne J, Larsen J, Shields M, Gonzales J, Shames D, Sato M, et al. NeuroD1 regulates survival and migration of neuroendocrine lung carcinomas via signaling molecules TrkB and NCAM. Proc Natl Acad Sci U S A. 2013;110:6524-9 pubmed publisher
Melemedjian O, Tillu D, Asiedu M, Mandell E, Moy J, Blute V, et al. BDNF regulates atypical PKC at spinal synapses to initiate and maintain a centralized chronic pain state. Mol Pain. 2013;9:12 pubmed publisher
DeWitt J, Ochoa V, Urschitz J, Elston M, Moisyadi S, Nishi R. Constitutively active TrkB confers an aggressive transformed phenotype to a neural crest-derived cell line. Oncogene. 2014;33:977-85 pubmed publisher
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Whitfield T, Shi X, Sun W, McGinty J. The suppressive effect of an intra-prefrontal cortical infusion of BDNF on cocaine-seeking is Trk receptor and extracellular signal-regulated protein kinase mitogen-activated protein kinase dependent. J Neurosci. 2011;31:834-42 pubmed publisher
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product information
SKU :
248-BD-005/CF
Product Name :
BDNF Protein
Size :
5 ug (also 250 ug, 10 ug, 25 ug)
Description :
The Recombinant Human BDNF Protein from R&D Systems is derived from Sf 21 (baculovirus). The Recombinant Human BDNF Protein has been validated for the following applications: Bioactivity.
Target :
BDNF
Category :
Proteins and Enzymes
Buffer :
Lyophilized from a 0.2 m filtered solution in Sodium Citrate and NaCl.
Conjugate :
Unconjugated
Purity :
>97%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Species Reactivity :
Human, Mouse, Rat, Canine, Equine
Observed Molecular Weight :
13-14 kDa, reducing conditions
Theoretical Molecular Weight :
13.5 kDa
Gene Symbol :
BDNF
Details of Functionality :
Measured in a cell proliferation assay using BaF mouse pro-B cells transfected with TrkB. The ED50 for this effect is 0.2-2 ng/mL. The specific activity of Recombinant Human BDNF is approximately 1.3 x 103 units/╡g, which is calibrated against recombinant human BDNF WHO Standard (NIBSC code: 96/534). Measured by its binding ability in a functional ELISA. When Recombinant Human TrkB Fc Chimera (Catalog # 688-TK ) is coated at 1 ╡g/mL, Recombinant Human BDNF binds with an apparent Kd 1 nM.
Endotoxin Note :
<0.10 EU per 1 ╡g of the protein by the LAL method.
AccessionNumbers :
P23560
Applications :
Bioactivity
Source :
Sf 21 (baculovirus)-derived Recombinant Human BDNF Protein, CF
Price :
229 USD
Alternate Names :
abrineurin, ANON2, brain-derived neurotrophic factor, BULN2, MGC34632, neurotrophin
Storage :
Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 ░C as supplied. 1 month, 2 to 8 ░C under sterile conditions after reconstitution. 3 months, -20 to -70 ░C under sterile conditions after reconstitution.
company information
R&D Systems
614 McKinley Place N.E.
Minneapolis, MN 55413
info@RnDSystems.com
https://www.rndsystems.com
800 343-7475
headquarters: USA
R&D Systems develops and manufactures high-quality proteins and serves as a world leader in immunoassays. R&D Systems also produces quality antibodies, antibody arrays, stem cell and cell culture products, and cell selection and detection products, serving the life science and diagnostics industry.