Mouse Monoclonal Antibody to GSK3B | ProMab 30006 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

ProMab

product type :

antibody

product name :

Mouse Monoclonal Antibody to GSK3B

catalog :

30006

quantity :

100μl

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

3D10

The same clone is also sold as:

Novus Biologicals: NBP1-47470, NBP2-80757
Invitrogen: MA5-15597
Cell Signaling Technology: 9832
OriGene: TA336772
Abnova: MAB10672

reactivity :

human, mouse, rat

application :

western blot, ELISA, immunohistochemistry, immunocytochemistry, flow cytometry

product information

Catalog :

30006

Title :

Mouse Monoclonal Antibody to GSK3B

Species :

Human; Mouse; Monkey; Rat;

Clone :

3D10;

lsotype :

Mouse IgG2a

Size :

100μl

Storage :

4℃; -20℃ for long term storage

Description :

Glycogen synthase kinase 3 (GSK-3), a serine-threonine kinase with two isoforms (alpha and beta), was originally discovered as a key enzyme in glycogen metabolism. GSK-3 was subsequently shown to function in cell division, proliferation, motility and survival. GSK-3 plays a role in a number of pathological conditions including cancer and diabetes and is increasingly seen as an important component of neurological diseases. GSK-3 phosphorylates tau and presenilin-1, which are involved in the development of Alzheimer's disease. Both isoforms of GSK-3 are ubiquitously expressed, although particularly high levels of GSK-3beta are found in the brain where it is involved in synaptic plasticity, possibly via regulation of NMDA receptor trafficking. GSK-3 phosphorylates over 40 different substrates including signaling proteins, transcription factors and structural proteins, and is part of the signal transduction cascade of a large number of growth factors and cytokines. The activity of GSK is regulated by phosphorylation (Akt: Akt-mediated phosphorylation at Ser21 of GSK-3α and Ser9 of GSK-3β, S6K, RSK, PKA and PKC), dephosphorylation (PP1 and PP2A), and by binding to protein complexes (with beta-catenin, axin, CK1 and the APC complex).

Immunogen :

Purified recombinant fragment of human GSK3B expressed in E. Coli.;

Formulation :

Ascitic fluid containing 0.03% sodium azide.

Applications :

ELISA: 1/10000; WB: 1/500 - 1/2000; IHC: 1/200 - 1/1000; ICC: 1/200 - 1/1000; FCM: 1/200 - 1/400

References :

1. EMBO J. 1998 Mar 2;17(5):1371-84. ; ; 2. Curr Biol. 2001 Jan 9;11(1):44-9. ; ; 3. Cancer Lett. 2003 Sep 25;199(2):201-8.

Type :

Monoclonal Antibody

MW :

46kDa

Aliases :

GSK-3β; GSK3-beta