Mouse Monoclonal Antibody to NPT | ProMab 20057 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

ProMab

product type :

antibody

product name :

Mouse Monoclonal Antibody to NPT

catalog :

20057

quantity :

100µl

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

4B4D1

reactivity :

human

application :

western blot, ELISA

product information

Catalog :

20057

Title :

Mouse Monoclonal Antibody to NPT

Species :

Human

Clone :

4B4D1;

lsotype :

Mouse IgG2a

Size :

100µl

Storage :

4?; -20? for long term storage

Description :

Neomycin phosphotransferase II (nptII) gene is used in selection of transformed organisms. It was initially isolated from the transposon Tn5 that was present in the bacterium strain Escherichia coli K12. The gene codes for the aminoglycoside 3'-phosphotransferase (denoted aph(3')-II or NPTII) enzyme. NPTII is probably the most widely used selectable marker for plant transformation. It is also used in gene expression and regulation studies in different organisms in part because N-terminal fusions can be constructed that retain enzymatic activity. In animal cells, G418 and neomycin are used as selectable agents.

Immunogen :

Purified recombinant fragment of NPT expressed in E. Coli.

Formulation :

Ascitic fluid containing 0.03% sodium azide.

Applications :

ELISA: 1/10000; WB: 1/500 - 1/2000

References :

1. Landis WG et.al Human and ecological Risk Assessment 6(5): 875-899. ; 2. Stewart CN et.al BioTechniques 29(4): 832-843. ; 3. Thomson JA et.al Journal of Food Science 66(2): 188-193. ;

Type :

Monoclonal Antibody