product summary
request information :
company name :
Novus Biologicals
product type :
antibody
product name :
Rabbit Polyclonal Von Hippel Lindau Antibody
catalog :
NB100-488G
quantity :
0.1 ml
price :
315.00 USD
clonality :
polyclonal
host :
rabbit
reactivity :
human, rat
application :
western blot
more info or order :
Novus Biologicals product webpage
product information
Catalog Number :
NB100-488G
Product Name :
Rabbit Polyclonal Von Hippel Lindau Antibody
List Price :
315.00 USD
Product Type :
Primary Antibodies
Package Size :
0.1 ml
AltNames :
elongin binding protein, HRCA1, Protein G7, pVHL, RCA1, VHL1, von Hippel-Lindau disease tumor suppressor, von Hippel-Lindau syndrome, von Hippel-Lindau tumor suppressor
Background :
The von Hippel-Lindau (VHL) protein is a tumor suppressor that is highly conserved. VHL binds to transcription factor Elongin B and C subunits, and appears to prevent Elongin A from associating with the B and C subunits, thereby inhibiting the transcriptional activity of Elongin. The ability of VHL to block Elongin function may play a role in normal cell growth regulation. Mutated VHL sequences lose their ability to bind to Elongin subunits B and C, suggesting that the tumor suppression function of VHL may be linked to its ability to bind to Elongin. Likewise, the introduction of wild-type VHL suppresses growth in tumor cell lines lacking normal expression of VHL genes. The product of the VHL gene has multiple reported functions, the best characterized of which is its role as the recognition component of an ubiquitin E3 ligase complex responsible for mediating oxygen-dependent destruction of hypoxia-inducible factor-alpha (HIF-alpha) subunits.
Buffer :
50mM sodium borate
Clonality :
Polyclonal
Concentration :
1.0 mg/ml
Conjugate :
DyLight 488
Application Summary :
Western Blot
Host :
Rabbit
Immunogen :
A synthetic peptide derived from the mouse VHL protein (human sequence is conserved but not identical).
Preservative :
No Preservative
Purity :
Protein A purified
CrossReactivity :
This antibody reacts with the rat VHL protein. Other species have not been tested.
Storage :
Store at 4C in the dark.
Uses :
This antibody is useful for Western blot where a band ~21kDa is seen. The theoretical molecular weight of VHL is 24 kDa. However, different migrating species ranging from 21-30 kDa have been observed using antibodies to VHL, and may result from a variety of factors including alternatively spliced VHL mRNAs and protein degradation. Other applications have not been tested. Suggested working dilutions: * Western Blot - 1:500 to 1:1,000 (ECL) *Optimal working dilutions should be determined by the investigator.
Research Area :
Apoptosis, Cancer, HIF Target Genes, Hypoxia, Neuronal Cell Markers, Neuronal Stem Cell Markers
more info or order :
Novus Biologicals product webpage
company information
Novus Biologicals
8100 Southpark Way, A-8
Littleton, CO 80120
novus@novusbio.com
www.novusbio.com
3037301950
headquarters: usa
The mission of Novus Biologicals, LLC is to accelerate scientific discovery by developing and marketing unique products in the forefront of science. Novus Biologicals was organized to provide the biological research community with a mechanism for commercializing unique biological materials. We also focus on continually monitoring scientific trends and supply materials to serve these trends. By making these products widely available to institutional and commercial researchers, Novus Biologicals plays an important role in furthering biological research. All of Novus Biologicals products are supplied with detailed technical information and ongoing support. By serving niche and emerging markets, Novus Biologicals has become specialists in markets and has formed and will continue to form ongoing partnerships with our customers.