ELISA/assay

Mouse Death Associated Protein 6 ELISA Kit

MBS720068

96 Strip Wells

675 USD

ELISA Kit

Mouse Death Associated Protein 6 ELISA Kit

Death Associated Protein 6

death domain-associated protein 6 isoform a; Death domain-associated protein 6; death domain-associated protein 6; Fas-binding protein; CENP-C binding protein; ETS1-associated protein 1; death-associated protein 6; fas death domain-associated protein; death-domain associated protein; Daxx; hDaxx; ETS1-associated protein 1; EAP1; Fas death domain-associated protein

DAXX

DAXX; DAXX; DAP6; EAP1; BING2; BING2; DAP6; hDaxx; EAP1

DAXX_HUMAN

Mouse

This assay has high sensitivity and excellent specificity for detection of DAP6. No significant cross-reactivity or interference between DAP6 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between DAP6 and all the analogues, therefore, cross reaction may still exist in some cases.

Store all reagents at 2-8 degree C.

Samples: Serum, plasma, cell culture supernatants, body fluid and tissue homogenate. Intended Uses: This DAP6 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Mouse DAP6. This ELISA kit for research use only, not for therapeutic or diagnostic applications!

Sensitivity: The sensitivity in this assay is 1.0 pg/mL.

Cell Biology

Principle of the assay: DAP6 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-DAP6 antibody and an DAP6-HRP conjugate. The assay sample and buffer are incubated together with DAP6-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the DAP6 concentration since DAP6 from samples and DAP6-HRP conjugate compete for the anti-DAP6 antibody binding site. Since the number of sites is limited, as more sites are occupied by DAP6 from the sample, fewer sites are left to bind DAP6-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The DAP6 concentration in each sample is interpolated from this standard curve.

215422388

NP_001135441.1

NM_001141969.1

73,589 Da

Amyotrophic Lateral Sclerosis (ALS) Pathway 920975!!Amyotrophic Lateral Sclerosis (ALS) Pathway 83099!!Amyotrophic Lateral Sclerosis (ALS) Pathway 511!!Androgen Receptor Signaling Pathway 198806!!Apoptosis Modulation And Signaling Pathway 198822!!FAS Pathway And Stress Induction Of HSP Regulation 198894!!HIV-1 Nef: Negative Effector Of Fas And TNF-alpha Pathway 137995!!Herpes Simplex Infection Pathway 377873!!Herpes Simplex Infection Pathway 377865!!IL-6 Signaling Pathway 198864

This gene encodes a multifunctional protein that resides in multiple locations in the nucleus and in the cytoplasm. It interacts with a wide variety of proteins, such as apoptosis antigen Fas, centromere protein C, and transcription factor erythroblastosis virus E26 oncogene homolog 1. In the nucleus, the encoded protein functions as a potent transcription repressor that binds to sumoylated transcription factors. Its repression can be relieved by the sequestration of this protein into promyelocytic leukemia nuclear bodies or nucleoli. This protein also associates with centromeres in G2 phase. In the cytoplasm, the encoded protein may function to regulate apoptosis. The subcellular localization and function of this protein are modulated by post-translational modifications, including sumoylation, phosphorylation and polyubiquitination. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Nov 2008]

96 Strip Wells

675 USD