This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
MyBioSource
product type :
antibody
product name :
Gamma Glutamylcysteine Synthetase (GCS, Glutamate-Cysteine Ligase)
catalog :
MBS613710
quantity :
0.5 ml
price :
610 USD
clonality :
polyclonal
host :
rabbit
reactivity :
Laccaria bicolor S238N-H82
application :
western blot, immunoprecipitation
product information
catalog number :
MBS613710
products type :
Antibody
products full name :
Gamma Glutamylcysteine Synthetase (GCS, Glutamate-Cysteine Ligase)
products short name :
Gamma Glutamylcysteine Synthetase
products name syn :
Anti -Gamma Glutamylcysteine Synthetase (GCS, Glutamate-Cysteine Ligase)
other names :
gamma glutamylcysteine synthetase; gamma glutamylcysteine synthetase
other gene names :
LbGCS
clonality :
Polyclonal
isotype :
IgG
host :
Host: Rabbit; Source: Rat
reactivity :
Human, Monkey, Mouse, Rat
specificity :
Recognizes the heavy subunit of rat Gamma Glutamylcysteine Synthetase at ~73kD. Species Crossreactivity: human, mouse, monkey and bovine (weak).
purity :
Affinity Purified. Purified by Protein A affinity chromatography.
form :
Supplied as a liquid in 10mM PBS, pH 7.4, 0.2% BSA, 0.09% sodium azide.
storage stability :
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
tested application :
Western Blot (WB), Immunoprecipitation (IP)
app notes :
Suitable for use in Western Blot and Immunoprecipitation. Dilution: Western Blot: 2.5-5ug/ml for 2 hours at RT. Immunoprecipitation: 10ug/mg protein lysate.
other info1 :
Immunogen: Synthetic peptide corresponding to aa295-313 of rat Gamma Glutamylcysteine Synthetase. Cellular Localization: Cytoplasmic.
other info2 :
Positive Control: Jurkat, MAD109, PC12 cells
products categories :
Antibodies; Abs to Enzymes, Synthetase
products description :
Glutathione (GSH) plays an important role in detoxification of oxidants and toxins from the cells. In most cells GSH is synthesized de novo in two steps catalysed by Gamma Glutamylcysteine Synthetase (GCS) / Glutamate-cystein Ligase and GSH synthetase. GCS catalyzes the first and rate limiting step and is important in GSH homeostasis. It is composed of two subunits: heavy and light. These subunits are coded by different genes which are controlled by different mechanisms. The heavy subunit carries the catalytic activity and can be inhibited through feedback mechanism by GSH. Binding of light subunit to heavy subunit reduces the Michaelis-Menten constant for glutamate to a physiological concentration of 1.2mM and increases the inhibitory constant for GSH.
ncbi gi num :
170093063
ncbi gb acc num :
XP_001877753.1
ncbi pathways :
Glutathione Metabolism Pathway 88534!!Glutathione Metabolism Pathway 343!!Metabolic Pathways 132976
size :
0.5 ml
price :
610 USD
company information
MyBioSource
P.O. Box 153308
San Diego, CA 92195-3308
sales@mybiosource.com
www.mybiosource.com
1-888-627-0165
headquarters: USA
MyBioSource, LLC was orginally founded in Vancouver by three enthusiastic scientists who are passionate about providing the world with the best reagents available. Together, they form a company with a big vision known as MyBioSource. MyBioSource is now located in San Diego, California, USA.

"MyBioSource's number 1 vision is to be the world's number 1 quality reagents provider."

Our goal is to provide researchers, scientists and customers alike with a one-stop-shop for all of their reagents needs, whether it is monoclonal antibody, polyclonal antibody, recombinant protein, peptide, etc...

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