MyBioSource

antibody

RIAM

MBS608382

0.1 ml

585 USD

monoclonal

rabbit

human

immunohistochemistry, western blot, flow cytometry, FACS

MBS608382

Antibody

RIAM

RIAM

Anti -RIAM

Rap1-interacting adaptor molecule; Amyloid beta A4 precursor protein-binding family B member 1-interacting protein; amyloid beta A4 precursor protein-binding family B member 1-interacting protein; PREL-1; RARP-1; OTTHUMP00000019344; proline-rich protein 73; proline rich EVH1 ligand 1; proline-rich EVH1 ligand 1; APBB1-interacting protein 1; Rap1-interacting adaptor molecule; Rap1-GTP-interacting adaptor molecule; rap1-GTP-interacting adapter molecule; retinoic acid-responsive proline-rich protein 1; amyloid beta (A4) precursor protein-binding, family B, member 1 interacting protein; APBB1-interacting protein 1; Proline-rich EVH1 ligand 1; PREL-1; Proline-rich protein 73; Rap1-GTP-interacting adapter molecule; RIAM; Retinoic acid-responsive proline-rich protein 1

APBB1IP; APBB1IP; RIAM; INAG1; PREL1; RARP1; PREL1; RARP1; RIAM

AB1IP_HUMAN

Monoclonal

IgG

9E519

Host: Rabbit; Source: Human

Human

Recognizes human RIAM at ~110kD.

Supernatant. Supernatant

Supplied as a liquid in 50mM Tris-Glycine, pH 7.4, 0.15M sodium chloride, 0.05% BSA, 0.01% sodium azide, 40% Glycerol.

May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS)

Suitable for use in Flow Cytometry, Western Blot and Immunohistochemistry. Dilution: Flow Cytometry: 1:50. Western Blot: 1:1000-1:2000. Immunohistochemistry: 1:100-1:250

Immunogen: Synthetic peptide corresponding to residues near the N-terminus of human RIAM.

Antibodies; Abs to GTPase, Rab, Ras, Rho Proteins

RIAM (a Rap1-GTP-interacting adapter molecule) defines a family of adaptor molecules that contain a RA-like (Ras association) domain, a PH (pleckstrin homology) domain, and various proline-rich motifs (1). RIAM directly binds to Ena/VASP proteins, important modulators of cell migration that localize to focal adhesions and stress fibers, and co-localizes with them at focal adhesions in response to Ras activation. Moreover, RIAM directly binds to activated Ras in a phosphoinositide-dependent manner. RIAM is believed to be the first direct link between Ras signalling and cytoskeletal remodelling via Ena/VASP proteins during cell migration and spreading (2).

26000235

Q7Z5R6

73,183 Da

Formation Of Platelet Plug Pathway 106029!!GRB2:SOS Provides Linkage To MAPK Signaling For Intergrins Pathway 106055!!Hemostasis Pathway 106028!!Integrin AlphaIIb Beta3 Signaling Pathway 106054!!Integrin Cell Surface Interactions Pathway 106110!!Platelet Aggregation (Plug Formation) Pathway 106053!!P130Cas Linkage To MAPK Signaling For Integrins Pathway 106056

Function: Appears to function in the signal transduction from Ras activation to actin cytoskeletal remodeling. Suppresses insulin-induced promoter activities through AP1 and SRE. Mediates Rap1-induced adhesion. Ref.1 Ref.2. Subunit structure: Interacts, through the N-terminal Pro-rich region, with the WW domain of APBB1. Interacts with RAP1A, PFN1, VASP and ENAH. Ref.2. Subcellular location: Cell membrane; Peripheral membrane protein. By similarity. Cell projection lamellipodium. By similarity. Cell junction focal adhesion. By similarity. Cytoplasm cytoskeleton. By similarity. Note: Colocalizes with ENA/VASP proteins at lamellipodia tips and focal adhesions, and F-actin at the leading edge. At the membrane surface, associates, via the PH domain, preferentially with the inositol phosphates, PtdIns5P and PtdIns3P. This binding appears to be necessary for the efficient interaction of the RA domain to Ras-GTPases. By similarity. Ref.2. Tissue specificity: Widely expressed with high expression in thymus, spleen, lymph node, bone marrow and peripheral leukocytes. Ref.1 Ref.2. Induction: By all-trans-retinoic acid (ATRA). Domain: The two Pro-rich regions are required for the suppression of AP1 transcription activity. Sequence similarities: Belongs to the MRL family.Contains 1 PH domain.Contains 1 Ras-associating domain.

0.1 ml

585 USD