catalog number :
MBS534779
products full name :
Myeloperoxidase antibody
products short name :
Myeloperoxidase
products name syn :
Polyclonal Myeloperoxidase; Anti-Myeloperoxidase; Myeloperoxidase; MPO; 89 kDa myeloperoxidase; 84 kDa myeloperoxidase; Myeloperoxidase light chainMyeloperoxidase heavy chain
other names :
myeloperoxidase; Myeloperoxidase; myeloperoxidase; myeloperoxidase
other gene names :
MPO; MPO; MPO
uniprot entry name :
PERM_HUMAN
reactivity :
To be determined by end-user
specificity :
Human myeloperoxidase
purity :
Myeloperoxidase antibody was purified by delipidation and defibrination.
form :
Lyophilized from 0.02M K3PO4, pH 7.2, with 0.15M NaCl, and 0.01%NaN3.
storage stability :
Store at 4 degree C until reconstitution. Following reconstitution aliquot and freeze at -20 degree C for long term storage. Avoid repeated freeze/thaw cycles.
tested application :
ELISA (EIA), Immunoprecipitation (IP), Western Blot (WB)
app notes :
ELISA: 1:200,000. IP: 1:100. WB: 1:5,000
other info1 :
Biological Significance: Human myeloperoxidase (MPO) is a dimeric protein composed of two heavy subunits (53 kDa) and two light subunits (15 kDa). Each MPO molecule contains two prosthetic porphyrins which play an important role in the catalytic cycle. Molecular weights for MPO isoforms from pools of normal human samples range from 114,000 to 140,000 daltons reflecting a heterogeneous mixture of isoforms when assayed under non-reducing conditions of SDS-PAGE. Often MPO from a single donor will yield a homogenous preparation reflecting a single isoform. The carbohydrate component of MPO, consisting of mannose, glucose and N-acetylglucosamine residues is 2.5%. MPO is inhibited by azide and other compounds. MPO is stored in primary granules of neutrophils and serves as a bactericidal agent in that MPO catalyzes the production of hypochlorous acid (HOCl), a powerful oxidant. HOCl is derived from chloride ion (Cl-) and hydrogen peroxide (H2O2). In a number of inflammatory situations, MPO is released into the extracellular matrix where its measurement can be used as an indication of neutrophil activation.
other info2 :
Biohazard Information: This product contains sodium azide as preservative. Although the amount of sodium azide is very small appropriate care must be taken when handling this product. Immunogen: Myeloperoxidase antibody was raised in rabbit using myeloperoxidase from human Leukocytes as the immunogen.
products categories :
Proteases, Inhibitors, & Enzymes
products description :
Rabbit polyclonal Myeloperoxidase antibody
ncbi acc num :
NP_000241.1
ncbi gb acc num :
NM_000250.1
ncbi mol weight :
87,248 Da
ncbi pathways :
C-MYB Transcription Factor Network Pathway 138073!!Folate Metabolism Pathway 198833!!IL23-mediated Signaling Events Pathway 138000!!Phagosome Pathway 153910!!Phagosome Pathway 153859!!Selenium Pathway 198825!!Transcriptional Misregulation In Cancer Pathway 523016!!Transcriptional Misregulation In Cancer Pathway 522987!!Vitamin B12 Metabolism Pathway 920974!!Amb2 Integrin Signaling Pathway 137945
ncbi summary :
Myeloperoxidase (MPO) is a heme protein synthesized during myeloid differentiation that constitutes the major component of neutrophil azurophilic granules. Produced as a single chain precursor, myeloperoxidase is subsequently cleaved into a light and heavy chain. The mature myeloperoxidase is a tetramer composed of 2 light chains and 2 heavy chains. This enzyme produces hypohalous acids central to the microbicidal activity of neutrophils. [provided by RefSeq, Nov 2014]