Vimentin antibody (biotin) | MyBioSource MBS531736 product information

product summary

request information :

company name :

MyBioSource

product type :

antibody

product name :

Vimentin antibody (biotin)

catalog :

MBS531736

quantity :

0.25 ml

price :

685 USD

clonality :

monoclonal

host :

mouse

conjugate :

biotin

reactivity :

dog, cow, human, chicken

application :

enzyme immunoassay, immunohistochemistry - paraffin section, FACS, ELISA, flow cytometry, immunohistochemistry

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS531736

products type :

Antibody

products full name :

Vimentin antibody (biotin)

products short name :

Vimentin (biotin)

products name syn :

Monoclonal Vimentin; Anti-Vimentin

other names :

vimentin; Vimentin; vimentin; vimentin; N/A

other gene names :

VIM; VIM; N/A

uniprot entry name :

VIME_HUMAN

clonality :

Monoclonal

isotype :

IgG2a

clone :

VIM 3B4

host :

Mouse

reactivity :

Human, monkey, bovine, dog, chicken, amphibia (e.g. Xenopus laevis); (weaker murine cross-reaction)

specificity :

Bovine vimentin

purity :

Protein A affinity chromatography

form :

Protein A affinity chromatography

concentration :

250 ul

storage stability :

Store at 4 deg C.

tested application :

ELISA (EIA), Flow Cytometry (FC), Immunohistochemistry (IHC) (Formalin/Paraffin), FACS, and cell sorting. Suitable for frozen and paraffin-embedded tissues and cytological material.

app notes :

IHC-F: 1:10, IHC-P: 1:10

other info1 :

Immunogen: Vimentin purified from bovine len. Tag Conjugate: Biotin

other info2 :

Biohazard Information: This product contains sodium azide as preservative. Although the amount of sodium azide is very small appropriate care must be taken when handling this product.

products categories :

Cell Biology; Monoclonal Antibodies; Conjugated Monoclonal Antibodies

products description :

Mouse monoclonal Vimentin antibody (biotin) . Vimentin is a member of the intermediate filament family of proteins that is especially found in connective tissue. Intermediate filaments are an important structural feature of eukaryotic cells. They, along with microtubules and actin microfilaments, make up the cytoskeleton. Although most intermediate filaments are stable structures, in fibroblasts, vimentin exists as a dynamic structure. This filament is used as a marker for mesodermally derived tissues, and as such can be used as an immunohistochemical marker for sarcomas.

ncbi gi num :

62414289

ncbi acc num :

NP_003371.2

ncbi gb acc num :

NM_003380.3

uniprot acc num :

P08670

ncbi mol weight :

53,652 Da

ncbi pathways :

Alpha6-Beta4 Integrin Signaling Pathway 198807!!Apoptosis Pathway 105648!!Apoptotic Cleavage Of Cellular Proteins Pathway 105678!!Apoptotic Execution Phase Pathway 105677!!Aurora B Signaling Pathway 138080!!Caspase Cascade In Apoptosis Pathway 137974!!Caspase-mediated Cleavage Of Cytoskeletal Proteins Pathway 105679!!Epstein-Barr Virus Infection Pathway 585562!!Epstein-Barr Virus Infection Pathway 587115!!Muscle Contraction Pathway 106261

ncbi summary :

This gene encodes a member of the intermediate filament family. Intermediate filamentents, along with microtubules and actin microfilaments, make up the cytoskeleton. The protein encoded by this gene is responsible for maintaining cell shape, integrity of the cytoplasm, and stabilizing cytoskeletal interactions. It is also involved in the immune response, and controls the transport of low-density lipoprotein (LDL)-derived cholesterol from a lysosome to the site of esterification. It functions as an organizer of a number of critical proteins involved in attachment, migration, and cell signaling. Mutations in this gene causes a dominant, pulverulent cataract.[provided by RefSeq, Jun 2009]

uniprot summary :

Function: Vimentins are class-III intermediate filaments found in various non-epithelial cells, especially mesenchymal cells. Vimentin is attached to the nucleus, endoplasmic reticulum, and mitochondria, either laterally or terminally. Ref.40Involved with LARP6 in the stabilization of type I collagen mRNAs for CO1A1 and CO1A2. Ref.40. Subunit structure: Homopolymer. Interacts with HCV core protein. Interacts with LGSN and SYNM. Interacts (via rod region) with PLEC (via CH 1 domain) . By similarity. Interacts with SLC6A4. Interacts with STK33. Interacts with LARP6. Interacts with RAB8B . By similarity. Ref.23 Ref.31 Ref.37 Ref.40. Subcellular location: Cytoplasm Ref.41. Tissue specificity: Highly expressed in fibroblasts, some expression in T- and B-lymphocytes, and little or no expression in Burkitt's lymphoma cell lines. Expressed in many hormone-independent mammary carcinoma cell lines. Ref.12 Ref.17. Post-translational modification: Filament disassembly during mitosis is promoted by phosphorylation at Ser-55 as well as by nestin . By similarity. One of the most prominent phosphoproteins in various cells of mesenchymal origin. Phosphorylation is enhanced during cell division, at which time vimentin filaments are significantly reorganized. Phosphorylation by PKN1 inhibits the formation of filaments. Phosphorylated at Ser-56 by CDK5 during neutrophil secretion in the cytoplasm. Phosphorylated by STK33. Ref.14 Ref.19 Ref.20 Ref.21 Ref.22 Ref.24 Ref.25 Ref.26 Ref.27 Ref.28 Ref.29 Ref.30 Ref.31 Ref.32 Ref.33 Ref.34 Ref.35 Ref.36 Ref.38 Ref.41. Sequence similarities: Belongs to the intermediate filament family. Sequence caution: The sequence BAB71275.1 differs from that shown. Reason: Intron retention.

size :

0.25 ml

price :

685 USD

more info or order :

MyBioSource product webpage