Sheep anti-human Factor XII (F.XII), Whole IgG from antiserum | MyBioSource MBS512073 product information

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product summary

company name :

MyBioSource

product type :

antibody

product name :

Sheep anti-human Factor XII (F.XII), Whole IgG from antiserum

catalog :

MBS512073

quantity :

5 mg

price :

280 USD

clonality :

polyclonal

host :

sheep

reactivity :

human

product information

catalog number :

MBS512073

products type :

Antibody

products full name :

Sheep anti-human Factor XII (F.XII), Whole IgG from antiserum

products short name :

Factor XII

products name syn :

Factor XII, human

other names :

factor XII; Factor XII; coagulation factor XII; Hageman factor; OTTHUMP00000223845; beta-factor XIIa part 1; beta-factor XIIa part 2; coagulation factor XIIa heavy chain; coagulation factor XIIa light chain; coagulation factor XII (Hageman factor)

other gene names :

F12; HAF; HAE3; HAEX

uniprot entry name :

P78343_HUMAN

clonality :

Polyclonal

host :

Sheep

specificity :

This antibody is specific for factor XII as demonstrated by immunoelectrophoresis and ELISA.

purity :

Purified IgG. Vial containing ml of whole IgG representing approximately 1 ml of antiserum. Total protein is 5 mg.

form :

Whole IgG, clear liquid.

concentration :

IgG concentration is mg/ml, determined by absorbance using an extinction coefficient (E1%280) of 13.4.

storage stability :

Store between -10 and -20 degree C. Product will become viscous but will not freeze. Avoid storage in frost-free freezers. Keep vial tightly capped. Allow product to warm to room temperature and gently mix before use.

app notes :

Suitable as a source of antibodies to human factor XII.

other info1 :

Immunogen: Human Factor XII purified from plasma.

other info2 :

Buffer: 10 mM HEPES, pH 7.4, 150 mM NaCl, 50% (v/v) glycerol. Neutralizing Activity: Not determined

products description :

Factor XII (F.XII, Hageman factor) is a 76 kDa, single chain glycoprotein produced in the liver. In plasma, F.XII circulates as a protease zymogen at a concentration of approximately 30 ug/ml (400 nM). Upon vascular injury F.XII binds to negatively charged extravascular surfaces such as cartilage and skin, which facilitate activation of the zymogen to the active serine protease. Cleavage of F.XII by kallikrein after residue Arg353 produces the enzyme alphaF.XIIa, consisting of a 28 kDa light chain containing the protease domain, and a 52 kDa heavy chain containing the anionic surface-binding domain. Substrates for surface bound F.XIIa include the zymogens prekallikrein (PK) and factor XI (F.XI) as well as the procofactor high-molecular weight kininogen (HK). The activation of these substrates results in positive feedback activation of F.XII. Further cleavage of alphaF.XIIa by kallikrein produces the 28 kDa fragment betaF.XIIa (Hageman factor fragment). betaF.XIIa has reduced procoagulant activity as it lacks the anionic surface-binding domain, but is capable of fluid-phase activation of PK, factor VII and complement C1. The activity of F.XIIa in plasma is regulated predominantly by C1-Inhibitor, with relatively minor contributions by alpha2antiplasmin, alpha2macroglobulin and antithrombin, even in the presence of therapeutic levels of heparin1-3.

products references :

1. DeLa Cadena R, Watchtfogel YT, Colman RW, in Hemostasis and Thrombosis, 3rd Edition, eds. RW Colman, J Hirsh, VJ Marder and EW Salzman, pp. 219-240, J.B. Lippincott Co., Philadelphia, 1994. 2. Tankersley DL, Alving BM, Finlayson JS; Preparation of betaF.XIIa (Hageman Factor Fragment) from Human Plasma. Thrombosis Research 25 pp 307- 317, 1982. 3. Pixley RA, Schapira M, Coleman RW; The regulation of Human Factor XIIa by Plasma Proteinase Inhibitors. JBC 260, pp 1723-1729, 1985.

ncbi gi num :

1857200

uniprot acc num :

P78343

ncbi mol weight :

3,577 Da

ncbi pathways :

Blood Clotting Cascade Pathway 198840!!Complement And Coagulation Cascades Pathway 198880!!Complement And Coagulation Cascades Pathway 83073!!Complement And Coagulation Cascades Pathway 484!!Formation Of Fibrin Clot (Clotting Cascade) Pathway 106057!!Hemostasis Pathway 106028!!Intrinsic Pathway 106059

ncbi summary :

This gene encodes coagulation factor XII which circulates in blood as a zymogen. This single chain zymogen is converted to a two-chain serine protease with an heavy chain (alpha-factor XIIa) and a light chain. The heavy chain contains two fibronectin-type domains, two epidermal growth factor (EGF)-like domains, a kringle domain and a proline-rich domain, whereas the light chain contains only a catalytic domain. On activation, further cleavages takes place in the heavy chain, resulting in the production of beta-factor XIIa light chain and the alpha-factor XIIa light chain becomes beta-factor XIIa heavy chain. Prekallikrein is cleaved by factor XII to form kallikrein, which then cleaves factor XII first to alpha-factor XIIa and then to beta-factor XIIa. The active factor XIIa participates in the initiation of blood coagulation, fibrinolysis, and the generation of bradykinin and angiotensin. It activates coagulation factors VII and XI. Defects in this gene do not cause any clinical symptoms and the sole effect is that whole-blood clotting time is prolonged. [provided by RefSeq]

size :

5 mg

price :

280 USD