antibody

Neuronal Nitric Oxide Synthase

MBS472037

0.1 ml

280 USD

polyclonal

rabbit

nonconjugated

human, mouse, rat

western blot, ELISA

Antibody

Neuronal Nitric Oxide Synthase

Neuronal Nitric Oxide Synthase

nNOS; NOS type I; Constitutive NOS; neuronal nitric oxide synthase; NC-NOS

neuronal nitric oxide synthase; Neuronal nitric-oxide synthase isoform mu; nitric oxide synthase, brain; NOS type I; neuronal NOS; constitutive NOS; OTTHUMP00000174427; OTTHUMP00000174428; peptidyl-cysteine S-nitrosylase NOS1; nitric oxide synthase 1 (neuronal)

NOS1; nNOSmu; NOS; bNOS; nNOS; IHPS1; N-NOS; NC-NOS

O75713_HUMAN

Polyclonal

Rabbit

Human, Rat, Mouse

The antibody detects a 155 kDa* protein on SDS-PAGE immunoblots of rat and mouse brain tissue lysates.

Affinity Purified

Rabbit polyclonal, affinity-purified antibody is supplied in 100 ul phosphate-buffered saline, 50% glycerol, 1 mg/ml BSA, and 0.05% sodium azide.

Store at -20 degree C. Do not aliquot. Stable for 1 year.

ELISA, Western Blot

ELISA: 1:2000. Western Blot: 1:1000

Immunogen: Amino acid residues within the C-terminal region of human nNOS. The human nNOS sequence used has high homology with similar regions in rat and mouse nNOS.

Nitric oxide (NO) has a broad range of biological activities and is implicated in signaling pathways in phylogenetically diverse species. Nitric oxide synthases (NOS), the enzymes responsible for synthesis of NO, are homodimers whose monomers are themselves two fused enzymes: a cytochrome reductase and a cytochrome that requires three cosubstrates (L-arginine, NADPH, and O2) and five cofactors or prosthetic groups (FAD, FMN, calmodulin, tetrahydrobiopterin, and heme). Several distinct NOS isoforms are produced from three distinct genes. These include two constitutive Ca2+/CaM-dependent forms of NOS: nNOS (also designated bNOS, NOS-I), whose activity was first identified in neurons and eNOS (also designated ecNOS, NOS-III) first identified in endothelial cells. The inducible form of NOS, iNOS (also designated NOS-II), is Ca2+ independent and is expressed in a broad range of cell types. This form of NOS is induced after stimulation with cytokines and exposure to microbial products.

Kleinert, H. et al. (2003) Biol Chem. 384(10-11):1343. Xie, Q.W. et al. (1992) Science 256:225.

642518

O75713

7,497 Da

Alzheimer's Disease Pathway 83097!!Alzheimer's Disease Pathway 509!!Amyotrophic Lateral Sclerosis (ALS) Pathway 83099!!Amyotrophic Lateral Sclerosis (ALS) Pathway 511!!Arginine And Proline Metabolism Pathway 82957!!Arginine And Proline Metabolism Pathway 323!!Calcium Signaling Pathway 83050!!Calcium Signaling Pathway 459!!Formation Of Platelet Plug Pathway 106029!!Hemostasis Pathway 106028

The protein encoded by this gene belongs to the family of nitric oxide synthases, which synthesize nitric oxide from L-arginine. Nitric oxide is a reactive free radical, which acts as a biologic mediator in several processes, including neurotransmission, and antimicrobial and antitumoral activities. In the brain and peripheral nervous system, nitric oxide displays many properties of a neurotransmitter, and has been implicated in neurotoxicity associated with stroke and neurodegenerative diseases, neural regulation of smooth muscle, including peristalsis, and penile erection. This protein is ubiquitously expressed, with high level of expression in skeletal muscle. Multiple transcript variants that differ in the 5' UTR have been described for this gene but the full-length nature of these transcripts is not known. Additionally, alternatively spliced transcript variants encoding different isoforms (some testis-specific) have been found for this gene.

0.1 ml

280 USD