antibody

Unphosphorylated beta-III-Tubulin (Ser-444)

MBS472007

0.1 ml

380 USD

polyclonal

rabbit

nonconjugated

human, mouse

western blot, ELISA, immunocytochemistry

Antibody

Unphosphorylated beta-III-Tubulin (Ser-444)

beta-III-Tubulin (Ser-444)

Tubulin beta

Polyclonal

Rabbit

Human, Rat, Mouse

This antibody was cross-adsorbed to phospho-bIIITubulin (Ser-444) peptide before affinity purification using unphosphorylated bIII-Tubulin (Ser-444) peptide (without carrier). The antibody detects a 50 kDa* protein corresponding to the molecular mass of unphosphorylated bIII-Tubulin on SDS-PAGE immunoblots of purified brain tubulin and mouse brain tissue.

Affinity Purified

Rabbit polyclonal, affinity-purified antibody is supplied in 100 ul phosphate-buffered saline, 50% glycerol, 1 mg/ml BSA, and 0.05% sodium azide.

Store at -20 degree C. Do not aliquot. Stable for 1 year.

ELISA, Western Blot, Immunocytochemistry

ELISA: 1:2000. Immunocytochemistry: 1:100. Western Blot: 1:1000

Immunogen: Unphosphorylated bIII-Tubulin (Ser-444) synthetic peptide (coupled to KLH) corresponding to amino acid residues around serine 444 of human bIII-tubulin. This sequence is not found in bI or bII-tubulin isotypes, but is well conserved in bIII-tubulins from rat and mouse.

Microtubules (MTs) are cytoskeletal elements that play an essential role in cell division and cytoplasmic organization. MTs are dynamic polymers of a/b-tubulin heterodimers. At least two populations of MTs, called dynamic and stable according to their rates of turnover, are readily distinguishable in cells. The proteins associated with MTs (MAPs) are among the bestknown factors that regulate MT dynamics and stability. In addition, a variety of different post-translational modifications may also regulate MT dynamics and stability. Phosphorylation is one of these modifications and it can occur on serine, threonine, and tyrosine residues in b-tubulin isoforms. Multiple kinases can phosphorylate Ser-444 at the C-terminus of bIII-tubulin in vitro. Unphosphorylated Ser-444 in bIII-tubulin is an early marker for cells of neuronal lineage, while phosphorylation of Ser-444 is upregulated after neuronal maturation and may preferentially occur in assembled MTs. By contrast, Cdk1 phosphorylation of Ser-172 in btubulin occurs in mitotic cells and may impair tubulin incorporation into microtubules.

Fourest-Lieuvin, A. et al. (2006) Mol. Biol. Cell. 17(3):1041. Westermann, S. & Weber, K. (2003) Nat. Rev. Mol. Cell. Biol. 4:938. Fanarraga, M.L. et al. (1999) Eur. J. Neurosci. 11:517. Diaz-Nido, J. et al. (1990) J Biol. Chem. 265(23):13949.

0.1 ml

380 USD