antibody

Rabbit anti GSK-3beta (Paired Ser9), Non-phospho

MBS462053

0.1 mg

265 USD

polyclonal

rabbit

nonconjugated

human, mouse, rat

western blot, ELISA, immunohistochemistry

Antibody

Rabbit anti GSK-3beta (Paired Ser9), Non-phospho

GSK3b (Paired S9)

Rabbit anti--GSK3b (Paired S9); Glycogen synthase kinase 3 beta (GSK3-b)

glycogen synthase kinase-3 beta isoform 1; Glycogen synthase kinase-3 beta; glycogen synthase kinase-3 beta; GSK-3 beta; GSK3beta isoform; OTTHUMP00000165239; OTTHUMP00000165240; glycogen synthase kinase 3 beta

GSK3b (Paired S9)

GSK3B; GSK3B; GSK3B

GSK3B_HUMAN

Rabbit

Human, Mouse, Rat

This antibody recognizes GSK-3beta (Paired Ser9) without phosphorylated site at Serine 9. It does not cross-react with phosphospecific peptides.

The Rabbit IgG is purified by site-modified Epitope Affinity Purification.

This affinity purified antibody is supplied in sterile Tris-buffered saline (pH7.2) containing antibody stabilizer

Size: 100 ug/200 ul

The antibodies are stable for 12 months from date of receipt when stored at -20 degree C to -70 degree C. The antibodies can be stored at 2 degree C-8 degree C for three month without detectable loss of activity. Avoid repeated freezing-thawing cycles.

ELISA, Western Blot, Immunohistochemistry

Western Blot: 0.1-1 ug/ml. ELISA: 0.01-0.1 ug/ml. Immunoprecipitation: 2-5 ug/ml

Antigen Preparation: A synthetic peptide surrounding to the epitope -TTSFA- without phosphorylation of human GSK3-beta protein. This sequence is identical among human, mouse, rat, bovine, dog and chicken species

Positive Control: 3T3 cell lysate. Cellular Location: Cytoplasmic

Glycogen Synthase Kinase-3beta (GSK-3beta) is a serine/threonine kinase that affects glycogen metabolism by phosphorylating and down-regulating the activity of muscle glycogen synthase. Growth factor (such as Insulin) binds to its receptor, recruits PKB/AKT kinase to phsphorylate GSK-3beta on Serine 9, which prevents GSK-3beta from phosphorylating glycogen synthase. In contrast, the phosphorylation of tyrosine 216 in the catalytic domain increase this kinase activity to promote cell death under the absence of growth factors. GSK-3beta is also involved in Wnt signaling pathway where it forms a complex with axin, beta-catenin and adenomatous polyposis coli (APC) protein, thus the GSK-3beta inhibitor is considered as a potential approach in Alzheimer's Disease (AD) treatment.

Alan P. Kozikowski, et al. Highly Potent and Specific GSK-3beta Inhibitors That Block Tau Phosphorylation and Decrease alpha- Synuclein Protein Expression in a Cellular Model of Parkinson's Disease. ChemMedChem, 2006, 1 (2), 256-266.

21361340

NP_002084.2

NM_002093.2

P49841

46 kDa

AKT Phosphorylates Targets In The Cytosol Pathway (106475); Alzheimer's Disease Pathway (83097); Alzheimer's Disease Pathway (509); Aurora A Signaling Pathway (137925); Axon Guidance Pathway (83065); Axon Guidance Pathway (476); Axon Guidance Pathway (105688); B Cell Receptor Signaling Pathway (198909); B Cell Receptor Signaling Pathway (83081); B Cell Receptor Signaling Pathway (492)

The protein encoded by this gene is a serine-threonine kinase, belonging to the glycogen synthase kinase subfamily. It is involved in energy metabolism, neuronal cell development, and body pattern formation. Polymorphisms in this gene have been implicated in modifying risk of Parkinson disease, and studies in mice show that overexpression of this gene may be relevant to the pathogenesis of Alzheimer disease. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.

Function: Participates in the Wnt signaling pathway. Implicated in the hormonal control of several regulatory proteins including glycogen synthase, MYB and the transcription factor JUN. Phosphorylates JUN at sites proximal to its DNA-binding domain, thereby reducing its affinity for DNA. Phosphorylates MUC1 in breast cancer cells, and decreases the interaction of MUC1 with CTNNB1/beta-catenin. Phosphorylates CTNNB1/beta-catenin. Phosphorylates SNAI1. Plays an important role in ERBB2-dependent stabilization of microtubules at the cell cortex. Prevents the phosphorylation of APC and CLASP2, allowing its association with the cell membrane. In turn, membrane-bound APC allows the localization of MACF1 to the cell membrane, which is required for microtubule capture and stabilization. Phosphorylates MACF1 and this phosphorylation inhibits the binding of MACF1 to microtubules which is critical for its role in bulge stem cell migration and skin wound repair. May be required for early embryo development and neuron differentiation. By similarity. Ref.7 Ref.11 Ref.30 Ref.34. Catalytic activity: ATP + [tau protein] = ADP + [tau protein] phosphate. Enzyme regulation: Activated by phosphorylation at Tyr-216. Inhibited when phosphorylated by AKT1. Ref.34. Subunit structure: Monomer. By similarity. Interacts with ARRB2 and DISC1. By similarity. Interacts with CABYR, MMP2, MUC1, NIN and PRUNE Interacts with AXIN1; the interaction mediates hyperphosphorylates of CTNNB1 leading to its ubiquitination and destruction. Interacts with and phosphorylates SNAI1. Interacts with DNM1L (via a C-terminal domain). Found in a complex composed of MACF1, APC, AXIN1, CTNNB1 and GSK3B. By similarity. Ref.6 Ref.7 Ref.9 Ref.10 Ref.11 Ref.13 Ref.18 Ref.20 Ref.27 Ref.34. Subcellular location: Cytoplasm. Nucleus. Cell membrane. Note: The phosphorylated form shows localization to cytoplasm and cell membrane. The MEMO1-RHOA-DIAPH1 signaling pathway controls localization of the phosophorylated form to the cell membrane. Ref.11 Ref.30. Tissue specificity: Expressed in testis, thymus, prostate and ovary and weakly expressed in lung, brain and kidney. Post-translational modification: Phosphorylated by AKT1 and ILK1. Activated by phosphorylation at Tyr-216. Ref.9 Ref.12 Ref.14 Ref.15 Ref.16 Ref.17 Ref.19 Ref.21 Ref.22 Ref.23 Ref.24 Ref.25 Ref.26 Ref.28 Ref.29 Ref.30 Ref.34. Sequence similarities: Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. GSK-3 subfamily.Contains 1 protein kinase domain.

0.1 mg

265 USD