catalog number :
MBS420748
products full name :
Goat anti-PODXL Antibody
products short name :
PODXL
products name syn :
PODXL; podocalyxin-like; Gp200; MGC138240; PCLP; PODXL; podocalyxin-like; Gp200; MGC138240; PCLP
other names :
podocalyxin isoform 1; Podocalyxin; podocalyxin; GCTM-2 antigen; podocalyxin-like protein 1; podocalyxin-like; GCTM-2 antigen; Gp200; Podocalyxin-like protein 1; PC; PCLP-1
products gene name :
PODXL
other gene names :
PODXL; PODXL; PC; PCLP; Gp200; PCLP-1; PCLP; PCLP1; PC; PCLP-1
uniprot entry name :
PODXL_HUMAN
reactivity :
Human, Mouse, Rat, Dog
sequence :
DNLTKDDLDEEEDTH
purity :
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide
form :
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
concentration :
100ug specific antibody in 200ul
storage stability :
Aliquot and store at -20 degree C. Minimize freezing and thawing.
tested application :
Peptide ELISA (EIA), Western Blot (WB)
app notes :
Western Blot: Approx 160kDa band observed in Human, Mouse and Rat Kidney lysates (calculated MW of 58.6kDa according to NP_001018121.1 and 55.4kDa according to NP_005388.2). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Kershaw et al., J Biol Chem. 1997 Jun 20;272(25):15708-14.; PMID: 9188463). Recommended concentration: 1-3ug/ml. Peptide ELISA: antibody detection limit dilution 1:8000.
other info1 :
Epitope: C Terminus. Immunogen: Peptide with sequence C-DNLTKDDLDEEEDTH, from the C Terminus of the protein sequence according to NP_001018121.1 ; NP_005388.2. Corresponding Immunizing Peptide: Catalog #MBS426053
other info2 :
Important Note: This antibody is expected to recognise both reported isoforms (NP_001018121.1 and NP_005388.2).
ncbi acc num :
NP_001018121.1
ncbi gb acc num :
NM_001018111.2
ncbi mol weight :
55,386 Da
ncbi summary :
This gene encodes a member of the sialomucin protein family. The encoded protein was originally identified as an important component of glomerular podocytes. Podocytes are highly differentiated epithelial cells with interdigitating foot processes covering the outer aspect of the glomerular basement membrane. Other biological activities of the encoded protein include: binding in a membrane protein complex with Na+/H+ exchanger regulatory factor to intracellular cytoskeletal elements, playing a role in hematopoetic cell differentiation, and being expressed in vascular endothelium cells and binding to L-selectin. [provided by RefSeq, Jul 2008]