MyBioSource

protein

Human Collagen Type I

MBS318088

0.5 mg

450 USD

MBS318088

Native Protein

Human Collagen Type I

Collagen Type I, Human

Purified Human Collagen, Type I

collagen, type I, alpha 1, isoform CRA_a; Collagen alpha-1(I) chain; collagen alpha-1(I) chain; collagen alpha-1(I) chain; OTTHUMP00000192905; alpha-1 type I collagen; pro-alpha-1 collagen type 1; collagen alpha 1 chain type I; collagen of skin, tendon and bone, alpha-1 chain; collagen, type I, alpha 1; Alpha-1 type I collagen

COL1A1; COL1A1; OI4

CO1A1_HUMAN

Placental villi

Collagen Type I, Human

>90% pure (SDS-PAGE). Controlled and limited pepsin digestion, followed by selective salt precipitation

Purified, Liquid

0.5mg/ml (determined by dry weight)

Warm temperatures cause collagen to denature which may appear as precipitation. Therefore we recommend dialysing in the cold for best results. May be transferred into physiological buffers by dialysis at 2 to 8 degree C. Purified collagen may be stored at

EIA/ELISA

Inactivation: Pepsin digestion and storage in 500mM acetic acid

Buffer: 500mM Acetic acid. Preservative: No. Warnings: All human sourced materials should be handled as if potentially infectious. Generally accepted laboratory practices appropriate for infectious materials should be employed when handling this product.

Purified Collagens

Miller, E.J. and Rhodes, R.K., 1982. Methods in Enzymology 82:33-64. Miller, E.J. In: Extracellular Matrix Biochemistry, K.A. Piez and A.H. Reddi, Eds. (Elsevier/New York, 1984), pp. 41-81.

119615037

P02452

138,941 Da

Amoebiasis Pathway 167324!!Amoebiasis Pathway 167191!!Axon Guidance Pathway 105688!!Cell Surface Interactions At The Vascular Wall Pathway 106062!!Collagen Adhesion Via GPVI Pathway 161058!!Collagen Adhesion Via Gp IV Pathway 106032!!Collagen Adhesion Via Alpha 2 Beta 1 Glycoprotein Pathway 106031!!ECM-receptor Interaction Pathway 83068!!ECM-receptor Interaction Pathway 479!!Focal Adhesion Pathway 198795

This gene encodes the pro-alpha1 chains of type I collagen whose triple helix comprises two alpha1 chains and one alpha2 chain. Type I is a fibril-forming collagen found in most connective tissues and is abundant in bone, cornea, dermis and tendon. Mutations in this gene are associated with osteogenesis imperfecta types I-IV, Ehlers-Danlos syndrome type VIIA, Ehlers-Danlos syndrome Classical type, Caffey Disease and idiopathic osteoporosis. Reciprocal translocations between chromosomes 17 and 22, where this gene and the gene for platelet-derived growth factor beta are located, are associated with a particular type of skin tumor called dermatofibrosarcoma protuberans, resulting from unregulated expression of the growth factor. Two transcripts, resulting from the use of alternate polyadenylation signals, have been identified for this gene. [provided by R. Dalgleish]

Function: Type I collagen is a member of group I collagen (fibrillar forming collagen). Subunit structure: Trimers of one alpha 2(I) and two alpha 1(I) chains. Interacts with MRC2. By similarity. Interacts with TRAM2. Ref.33. Subcellular location: Secreted extracellular space extracellular matrix. Tissue specificity: Forms the fibrils of tendon, ligaments and bones. In bones the fibrils are mineralized with calcium hydroxyapatite. Post-translational modification: Proline residues at the third position of the tripeptide repeating unit (G-X-Y) are hydroxylated in some or all of the chains. Proline residues at the second position of the tripeptide repeating unit (G-X-Y) are hydroxylated in some of the chains.O-linked glycan consists of a Glc-Gal disaccharide bound to the oxygen atom of a post-translationally added hydroxyl group. Involvement in disease: Defects in COL1A1 are the cause of Caffey disease (CAFFD) [. MIM:114000]; also known as infantile cortical hyperostosis. Caffey disease is characterized by an infantile episode of massive subperiosteal new bone formation that typically involves the diaphyses of the long bones, mandible, and clavicles. The involved bones may also appear inflamed, with painful swelling and systemic fever often accompanying the illness. The bone changes usually begin before 5 months of age and resolve before 2 years of age. Ref.89 Ref.90 Ref.91Defects in COL1A1 are a cause of Ehlers-Danlos syndrome type 1 (EDS1) [. MIM:130000]; also known as Ehlers-Danlos syndrome gravis. EDS is a connective tissue disorder characterized by hyperextensible skin, atrophic cutaneous scars due to tissue fragility and joint hyperlaxity. EDS1 is the severe form of classic Ehlers-Danlos syndrome. Ref.88 Ref.89 Ref.90 Ref.97Defects in COL1A1 are the cause of Ehlers-Danlos syndrome type 7A (EDS7A) [. MIM:130060]; also known as autosomal dominant Ehlers-Danlos syndrome type VII. EDS is a connective tissue disorder characterized by hyperextensible skin, atrophic cutaneous scars due to tissue fragility and joint hyperlaxity. EDS7A is marked by bilateral congenital hip dislocation, hyperlaxity of the joints, and recurrent partial dislocations. Ref.89 Ref.90Defects in COL1A1 are a cause of osteogenesis imperfecta type 1 (OI1) [. MIM:166200]. A dominantly inherited connective tissue disorder characterized by bone fragility and blue sclerae. Osteogenesis imperfecta type 1 is non-deforming with normal height or mild short stature, and no dentinogenesis imperfecta. Ref.38 Ref.44 Ref.55 Ref.59 Ref.62 Ref.68 Ref.89 Ref.90 Ref.93 Ref.94 Ref.96 Ref.99 Ref.101Defects in COL1A1 are a cause of osteogenesis imperfecta type 2A (OI2A) [. MIM:166210]; also known as osteogenesis imperfecta congenita. A connective tissue disorder characterized by bone fragility, with many perinatal fractures, severe bowing of long bones, undermineralization, and death in the perinatal period due to respiratory insufficiency.Defects in COL1A1 are a cause of osteogenesis imperfecta type 3 (OI3) [. MIM:259420]. A connective tissue disorder characterized by progressively deforming bones, very short stature, a triangular face, severe scoliosis, grayish sclera, and dentinogenesis imperfecta.Defects in COL1A1 are a cause of osteogenesis imperfecta type 4 (OI4) [. MIM:166220]; also known as osteogenesis imperfecta with normal sclerae. A connective tissue disorder characterized by moderately short stature, mild to moderate scoliosis, grayish or white sclera and dentinogenesis imperfecta.Genetic variations in COL1A1 are a cause of susceptibility to osteoporosis (OSTEOP) [. MIM:166710]; also known as involutional or senile osteoporosis or postmenopausal osteoporosis. Osteoporosis is characterized by reduced bone mass, disruption of bone microarchitecture without alteration in the composition of bone. Osteoporotic bones are more at risk of fracture. Ref.81 Ref.86 Ref.89 Ref.90Note=A chromosomal aberration involving COL1A1 is found in dermatofibrosarcoma protuberans. Translocation t(17;22)(q22;q13) with PDGF. Ref.89 Ref.90. Sequence similarities: Belongs to the fibrillar collagen family.Contains 1 fibrillar collagen NC1 domain.Contains 1 VWFC domain. Sequence caution: The sequence BAD92834.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.

0.5 mg

450 USD