catalog number :
MBS301548
products full name :
Mouse Anti-Human GP200 (Renal Cell Carcinoma Marker) Monoclonal Antibody (Clone SPM487)
products short name :
GP200
other names :
lymphocyte antigen 75; Lymphocyte antigen 75; lymphocyte antigen 75; C-type lectin domain family 13 member B; lymphocyte antigen 75; C-type lectin domain family 13 member B; DEC-205; gp200-MR6; CD_antigen: CD205
products gene name :
GP200
other gene names :
LY75; LY75; CD205; LY-75; CLEC13B; DEC-205; GP200-MR6; CD205; CLEC13B; Ly-75
uniprot entry name :
LY75_HUMAN
form :
0.1 ml mouse monoclonal antibody purified by protein A/G in PBS/1% BSA buffer pH 7.6 with less than 0.1% sodium azide.
storage stability :
Store at 2-8 degree C. Do not freeze. The user must validate any other storage conditions. When properly stored, the reagent is stable to the date indicated on the label. Do not use the reagent beyond the expiration date. There are no definitive signs to indicate instability of this product; therefore, positive and negative controls should be tested simultaneously with unknown specimens. If unexpected results are observed which cannot be explained by variations in laboratory procedures and a problem with the reagent is suspected, contact Technical Support.
tested application :
Immunohistochemistry (IHC) Paraffin
app notes :
Immunohistochemistry Procedure . Specimen Preparation: Formalin-fixed, paraffin-embedded tissues are suitable for use with this primary antibody. Deparaffinization: Deparaffinize slides using xylene or xylene alternative and graded alcohols. Antibody Dilution: If using the concentrate format of this product, dilute the antibody 1:100. The dilutions are estimates; actual results may differ because of variability in methods and protocols. Antigen Retrieval: Boil tissue section in 10mM citrate buffer, pH 6.0 for 10 min followed by cooling at RT for 20 min. Primary Antibody Incubation: Incubate for 30 minutes at RT. Slide Washing: Slides must be washed in between steps. Rinse slides with PBS/0.05% Tween. Visualization: Detect the antibody as instructed by the instructions provided with the visualization system. Immunohistochemistry Dilution: 1:100. IHC Positive Control: Normal Kidney, Renal Cell Carcinoma
other info2 :
Immunogen: Recombinant human gp200 protein. Control Tissue: Kidney, Renal Cell Carcinoma. Cellular Localization: Cytoplasm, Membrane. Entrez Gene Code: 4065. Pathway: Cancer Markers
products description :
In normal kidney, gp200 is localized along the brush border of the pars convoluta and pars recta segments of the proximal tubule, as well as focally along the luminal surface of Bowman's capsule adjoining the outgoing proximal tubule. Of other normal tissues examined, the gp200 is also localized along the luminal surfaces of breast lobules and ducts, the luminal surface of the epididymal tubular epithelium, within the cytoplasm of parathyroid parenchymal cells, and focally within the colloid of thyroid follicles. Thirty-one other normal tissues do not express similar or cross-reacting antigens. Reportedly, gp200 is expressed by 93% of primary and 84% of metastatic renal cell carcinomas.
ncbi acc num :
NP_002340.2
ncbi gb acc num :
NM_002349.3
ncbi mol weight :
~200 kDa
uniprot summary :
LY75: Acts as an endocytic receptor to direct captured antigens from the extracellular space to a specialized antigen- processing compartment. Causes reduced proliferation of B-lymphocytes. 5 isoforms of the human protein are produced by alternative splicing. Protein type: Membrane protein, integral. Chromosomal Location of Human Ortholog: 2q24. Cellular Component: integral to plasma membrane. Molecular Function: receptor activity; carbohydrate binding. Biological Process: immune response; endocytosis; inflammatory response
size1 :
0.1 mL (Concentrate)
size2 :
0.5 mL (Concentrate)
size4 :
1 mL (Concentrate)