Rabbit Anti-Human Adenomatous Polyposis Coli (APC) Polyclonal Antibody | MyBioSource MBS300691 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

MyBioSource

product type :

antibody

product name :

Rabbit Anti-Human Adenomatous Polyposis Coli (APC) Polyclonal Antibody

catalog :

MBS300691

quantity :

7 ml

price :

220 USD

clonality :

polyclonal

host :

rabbit

conjugate :

APC

reactivity :

human, mouse, rat

application :

immunohistochemistry, immunohistochemistry - paraffin section

product information

catalog number :

MBS300691

products type :

Antibody

products full name :

Rabbit Anti-Human Adenomatous Polyposis Coli (APC) Polyclonal Antibody

products short name :

Adenomatous Polyposis Coli

other names :

adenomatous polyposis coli protein isoform b; Adenomatous polyposis coli protein; adenomatous polyposis coli protein; adenomatosis polyposis coli tumor suppressor; deleted in polyposis 2.5; protein phosphatase 1, regulatory subunit 46; adenomatous polyposis coli; Deleted in polyposis 2.5

products gene name :

APC

other gene names :

APC; APC; GS; DP2; DP3; BTPS2; DP2.5; PPP1R46; DP2.5; Protein APC

uniprot entry name :

APC_HUMAN

clonality :

Polyclonal

isotype :

IgG

host :

Rabbit

reactivity :

Human. Predicted Reactivity: Frog, Mouse, Pig, Rat

sequence length :

2843

form :

7.0 ml pre-diluted immunogen affinity purified rabbit polyclonal antibody in TBS/1% BSA buffer pH 7.6 with less than 0.1% sodium azide.

storage stability :

Store at 2-8 degree C. Do not freeze. The user must validate any other storage conditions. When properly stored, the reagent is stable to the date indicated on the label. Do not use the reagent beyond the expiration date. There are no definitive signs to indicate instability of this product; therefore, positive and negative controls should be tested simultaneously with unknown specimens. If unexpected results are observed which cannot be explained by variations in laboratory procedures and a problem with the reagent is suspected, contact Technical Support.

tested application :

Immunohistochemistry (IHC) Paraffin

app notes :

Immunohistochemistry Procedure . Specimen Preparation: Formalin-fixed, paraffin-embedded tissues are suitable for use with this primary antibody. Deparaffinization: Deparaffinize slides using xylene or xylene alternative and graded alcohols. Antibody Dilution: If using the concentrate format of this product, dilute the antibody 1:100. The dilutions are estimates; actual results may differ because of variability in methods and protocols. Antigen Retrieval: Boil tissue sections in 10mM Citrate buffer, pH 6.0 for 10 min followed by cooling at room temperature for 20 min. Primary Antibody Incubation: Incubate for 10 minutes at room temperature. Slide Washing: Slides must be washed in between steps. Rinse slides with PBS/0.05% Tween. Visualization: Detect the antibody as instructed by the instructions provided with the visualization system. Immunohistochemistry Dilution: Ready To Use. IHC Positive Control: Colon, Colon Carcinoma

other info2 :

Immunogen: Synthetic peptide derived from C-terminus of human APC protein. Control Tissue: Colon, Colon Carcinoma. Cellular Localization: Cytoplasm. Entrez Gene Code: 324. Pathway: Tumor Suppressors

products description :

APC is associated with structural components of intracellular junctions, including conductin, and it competes with E-cadherin for binding to specific internal regions of both beta- and gemma-catenin. Like APC, APC2 contains SAMP domains, which are required for conductin binding. Both APC and APC2 regulate the formation of active beta-catenin-Tcf complexes.

ncbi gi num :

53759122

ncbi acc num :

NP_000029.2

ncbi gb acc num :

NM_000038.5

ncbi mol weight :

300 kDa

ncbi pathways :

AMER1 Mutants Destabilize The Destruction Complex Pathway 1127621!!APC Truncation Mutants Are Not K63 Polyubiquitinated Pathway 1127616!!APC Truncation Mutants Have Impaired AXIN Binding Pathway 1127617!!AXIN Missense Mutants Destabilize The Destruction Complex Pathway 1127620!!AXIN Mutants Destabilize The Destruction Complex, Activating WNT Signaling Pathway 1127618!!Apoptosis Pathway 105648!!Apoptotic Cleavage Of Cellular Proteins Pathway 105678!!Apoptotic Execution Phase Pathway 105677!!BDNF Signaling Pathway 712093!!Basal Cell Carcinoma Pathway 83113

ncbi summary :

This gene encodes a tumor suppressor protein that acts as an antagonist of the Wnt signaling pathway. It is also involved in other processes including cell migration and adhesion, transcriptional activation, and apoptosis. Defects in this gene cause familial adenomatous polyposis (FAP), an autosomal dominant pre-malignant disease that usually progresses to malignancy. Disease-associated mutations tend to be clustered in a small region designated the mutation cluster region (MCR) and result in a truncated protein product. [provided by RefSeq, Jul 2008]

size :

7 ml

price :

220 USD