catalog number :
MBS240510
products full name :
Rabbit Polyclonal to Human SPP1 / Osteopontin
products short name :
SPP1 / Osteopontin
products name syn :
Anti-SPP1 / Osteopontin Antibody (N-Terminus) IHC-plus; SPP1; BSPI; BNSP; Bone sialoprotein 1; ETA-1; Nephropontin; OPN; Secreted phosphoprotein 1; SPP-1; SPP1/CALPHA1 fusion; Urinary stone protein; Uropontin; Osteopontin; Bone sialoprotein I; Human SPP1; Osteopontin
other names :
osteopontin isoform OPN-b; Osteopontin; osteopontin; uropontin; nephropontin; SPP1/CALPHA1 fusion; urinary stone protein; early T-lymphocyte activation 1; osteopontin/immunoglobulin alpha 1 heavy chain constant region fusion protein; secreted phosphoprotein 1 (osteopontin, bone sialoprotein I, early T-lymphocyte activation 1); secreted phosphoprotein 1; Bone sialoprotein 1; Nephropontin; Secreted phosphoprotein 1; SPP-1; Urinary stone protein; Uropontin
products gene name :
SPP1
other gene names :
SPP1; SPP1; OPN; BNSP; BSPI; ETA-1; BNSP; OPN; SPP-1
uniprot entry name :
OSTP_HUMAN
reactivity :
Dog, Human, Mouse, Pig, Rat
specificity :
Synthetic peptide C-K-S-K-K-F-R-R-P-D-I-Q-Y-P-D corresponding to human osteopontin proteins conjugated to KLH using maleimide. A residue of cysteine was added to the amino terminal end to facilitate coupling.
purity :
Sterile filtered
form :
0.02 M potassium phosphate, 0.15 M sodium chloride, pH 7.2, 0.1% sodium azide.
storage stability :
Long term: -20 degree C; Short term: +4 degree C; Avoid freeze-thaw cycles.
tested application :
Immunohistochemistry (IHC - Paraffin), Western Blot (WB), ELISA (EIA)
app notes :
ELISA (1:20000 - 1:100000), IHC-P (1:500), WB (1:500 - 1:2000). Usage: Immunohistochemistry: was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antib.
other info1 :
Target Species: Human. Immunogen Description: Synthetic peptide from human SPP1 / Osteopontin.
other info2 :
Immunogen Type: Synthetic peptide. Immunogen: SPP1 / Osteopontin antibody was raised against synthetic peptide from human SPP1 / Osteopontin. Antigen Modification: N-Terminus
products description :
Osteopontin (OPN) is an integrin-binding protein overexpressed in various experimental models of malignancy and appears to be involved in tumorigenesis and metastasis.
ncbi acc num :
NP_000573.1
ncbi gb acc num :
NM_000582.2
ncbi mol weight :
33,843 Da
ncbi pathways :
BDNF Signaling Pathway 712093!!Degradation Of The Extracellular Matrix Pathway 576263!!Direct P53 Effectors Pathway 137939!!ECM-receptor Interaction Pathway 83068!!ECM-receptor Interaction Pathway 479!!Endochondral Ossification Pathway 198812!!Extracellular Matrix Organization Pathway 576262!!FGF Signaling Pathway 137989!!Focal Adhesion Pathway 198795!!Focal Adhesion Pathway 83067
ncbi summary :
The protein encoded by this gene is involved in the attachment of osteoclasts to the mineralized bone matrix. The encoded protein is secreted and binds hydroxyapatite with high affinity. The osteoclast vitronectin receptor is found in the cell membrane and may be involved in the binding to this protein. This protein is also a cytokine that upregulates expression of interferon-gamma and interleukin-12. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2011]