MOUSE ANTI HUMAN VINCULIN | MyBioSource MBS214146 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

MyBioSource

product type :

antibody

product name :

MOUSE ANTI HUMAN VINCULIN

catalog :

MBS214146

quantity :

2 mL

price :

405 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

V284

reactivity :

human

application :

western blot, immunocytochemistry, immunoprecipitation, flow cytometry, FACS

product information

catalog number :

MBS214146

products type :

Antibody

products full name :

MOUSE ANTI HUMAN VINCULIN

products short name :

VINCULIN

other names :

vinculin isoform VCL; Vinculin; vinculin; epididymis luminal protein 114; metavinculin; vinculin; Metavinculin; MV

other gene names :

VCL; VCL; MV; MVCL; CMD1W; CMH15; HEL114; MV

uniprot entry name :

VINC_HUMAN

clonality :

Monoclonal

isotype :

IgG1

clone :

V284

host :

Mouse

sequence length :

1066

specificity :

This item immunoblots vinculin on SDS gels and a 130kD band from homogenates of platelets and other cells. It reacts with leucocytes, muscle cells, epithelial cells and fibroblasts.

form :

Tissue Culture Supernatant containing 0.2M Tris/HCl pH7.4

storage stability :

Store at 4 degree C or at -20 degree C if preferred. This product should be stored undiluted. Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. Shelf Life: 18 months from date of despatch.

tested application :

Immunohistology Frozen, Flow cytometry (FC/FACS)*, Immunofluorescence (IF), Immunoprecipitation (IP), Immunohistology Paraffin*, Western Blot (WB)

app notes :

Flow Cytometry: Use 10ul of the suggested working dilution to label 1x106 cells in 100ul. Immunohistology - Frozen: Minimum Dilution: 1/10; Maximum Dilution: 1/50. Flow Cytometry: Maximum Dilution: 1/25; Application Note: Membrane permeabilisation is required for this application. MyBioSource recommends the use of Leucoperm for this purpose. . Immunohistology - Paraffin: Minimum Dilution: 1/10; Maximum Dilution: 1/50; Application Note: This product requires antigen retrieval using heat treatment prior to staining of paraffin sections. Sodium citrate buffer pH 6.0 is recommended for this purpose.

other info1 :

Perservative Stabilisers: 0.09% Sodium Azide

other info2 :

Immunogen: Purified Human Platelet Vinculin. Histology Positive Control Tissue: Human appendix. Fusion Partners: Spleen cells of immunised mice were fused with cells from the SP2/0 mouse myeloma line. Target Species: Human

products description :

MBS214146 immunoblots vinculin on SDS gels and a 130kD band from homogenates of platelets and other cells. It reacts with leucocytes, muscle cells, epithelial cells and fibroblasts.

ncbi gi num :

4507877

ncbi acc num :

NP_003364.1

ncbi gb acc num :

NM_003373.3

uniprot acc num :

P18206

ncbi mol weight :

24,904 Da

ncbi pathways :

Adherens Junction Pathway (83070); Adherens Junction Pathway (481); Amoebiasis Pathway (167324); Amoebiasis Pathway (167191); Bacterial Invasion Of Epithelial Cells Pathway (149807); Bacterial Invasion Of Epithelial Cells Pathway (148661); Focal Adhesion Pathway (198795); Focal Adhesion Pathway (83067); Focal Adhesion Pathway (478); Hemostasis Pathway (106028)

ncbi summary :

Vinculin is a cytoskeletal protein associated with cell-cell and cell-matrix junctions, where it is thought to function as one of several interacting proteins involved in anchoring F-actin to the membrane. Defects in VCL are the cause of cardiomyopathy dilated type 1W. Dilated cardiomyopathy is a disorder characterized by ventricular dilation and impaired systolic function, resulting in congestive heart failure and arrhythmia. Multiple alternatively spliced transcript variants have been found for this gene, but the biological validity of some variants has not been determined. [provided by RefSeq, Jul 2008]

uniprot summary :

Vinculin: Actin filament (F-actin)-binding protein involved in cell-matrix adhesion and cell-cell adhesion. Regulates cell- surface E-cadherin expression and potentiates mechanosensing by the E-cadherin complex. May also play important roles in cell morphology and locomotion. Exhibits self-association properties. Interacts with NRAP and SORBS1. Interacts with TLN1. Interacts with SYNM. Interacts with CTNNB1 and this interaction is necessary for its localization to the cell-cell junctions and for its function in regulating cell surface expression of E-cadherin. Metavinculin is muscle-specific. Belongs to the vinculin/alpha-catenin family. 3 isoforms of the human protein are produced by alternative splicing. Protein type: Motility/polarity/chemotaxis; Cytoskeletal; Cell adhesion. Chromosomal Location of Human Ortholog: 10q22.2. Cellular Component: focal adhesion; protein complex; costamere; extracellular region; intercellular junction; fascia adherens; cytosol; cell-matrix junction; actin cytoskeleton; cell-cell adherens junction; cytoskeleton; adherens junction; plasma membrane; vesicle. Molecular Function: protein binding; cadherin binding; beta-catenin binding; actin binding; structural molecule activity; alpha-catenin binding. Biological Process: lamellipodium biogenesis; platelet activation; platelet degranulation; apical junction assembly; muscle contraction; axon extension; cell-matrix adhesion; morphogenesis of an epithelium; cell adhesion; cell motility; blood coagulation; negative regulation of cell migration. Disease: Cardiomyopathy, Dilated, 1w

size1 :

2 mL

price1 :

405 USD