Chromogranin A antibody (clone 3A5) | MyBioSource MBS200121 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

MyBioSource

product type :

antibody

product name :

Chromogranin A antibody (clone 3A5)

catalog :

MBS200121

quantity :

0.05 mL

price :

240 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

3A5

reactivity :

human, mouse

application :

western blot, ELISA, immunohistochemistry, enzyme immunoassay

product information

catalog number :

MBS200121

products type :

Antibody

products full name :

Chromogranin A antibody (clone 3A5)

products short name :

Chromogranin A

products name syn :

CgA; CHGA; Pituitary secretory protein I (SP1); Chromogranin-A; beta Granin; CHG A; Chromogranin A precursor; SP I; Chromogranin A parathyroid secretory protein 1; ChromograninA; Pancreastatin; Parastatin; Parathyroid secretory protein 1; SP1; SPI; Vasostatin; Pituitary secretory protein I

other names :

chromogranin-A isoform 1 preproprotein; Chromogranin-A; chromogranin-A; SP-I; betagranin (N-terminal fragment of chromogranin A); catestatin; chromofungin; parathyroid secretory protein 1; pituitary secretory protein I; chromogranin A (parathyroid secretory protein 1); Pituitary secretory protein I; SP-I

other gene names :

CHGA; CHGA; CGA; CgA; SP-I

uniprot entry name :

CMGA_HUMAN

clonality :

Monoclonal

isotype :

IgG1, k

clone :

3A5

host :

Mouse

reactivity :

Human, Mouse

sequence length :

457

purity :

By protein-G affinity chromatography

form :

Liquid. Supplied in Phosphate-Buffered Saline (pH 7.4) with 0.1% Sodium Azide.

concentration :

1mg/ml

storage stability :

Can be stored at 4 degree C. For long term storage, aliquot and store at -20 degree C. Avoid repeated freezing and thawing cycles.

tested application :

ELISA (EIA), Western Blot (WB) (Tissue, Cell lysate), Immunohistochemistry (IHC)

app notes :

The antibody has been tested by ELISA and Western blot analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results. Recommended dilution range for Western blot analysis is 1:500 ~ 1,000. Recommended starting dilution is 1:500.

other info1 :

Antigen Species: Human. Clone: Anti-human Chromogranin A mAb, clone 3A5, is derived from hybridization of mouse F0 myeloma cells with spleen cells from BALB/c mice immunized with a recombinant human Chromogranin A protein. Immunogen: Recombinant human Chromogranin A (19-131aa) purified from E Coli

products categories :

Cancer

products description :

Chromogranin A (CGA) is the major member of the granin family of acidic secretory glycoproteins that are expressed in all endocrine and neuroendocrine cells. Chromogranin A regulates catecholamine storage and release through intracellular (vesiculogenic) and extracellular (catecholamine release-inhibitory) mechanisms. Chromogranin A also plays a role in targeting peptide hormones and neurotransmitters to granules of the regulated pathway by virtue of their ability to aggregate in the low-pH, high-calcium environment of the trans-Golgi network. Therefore, Chromogranin A represents a specific marker of neuroendocrine cells and tumours including pheochromocytomas.

products references :

Wen G, et al., (2004) Am J Hum Genet. 74(2):197-207 Nobels FR., et al., (1998) Eur J Ckin Invest. 28(6):431-40

ncbi gi num :

4502805

ncbi acc num :

NP_001266

ncbi gb acc num :

NM_001275.3

ncbi mol weight :

50,688 Da

ncbi summary :

The protein encoded by this gene is a member of the chromogranin/secretogranin family of neuroendocrine secretory proteins. It is found in secretory vesicles of neurons and endocrine cells. This gene product is a precursor to three biologically active peptides; vasostatin, pancreastatin, and parastatin. These peptides act as autocrine or paracrine negative modulators of the neuroendocrine system. Two other peptides, catestatin and chromofungin, have antimicrobial activity and antifungal activity, respectively. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2014]

uniprot summary :

CHGA: a member of the chromogranin/secretogranin family of neuroendocrine secretory proteins. It is found in secretory vesicles of neurons and endocrine cells and is a precursor to multiple biologically active peptides including vasostatin, pancreastatin, and chromostatin. Binds calcium with a low-affinity. Vasostatin negatively regulates angiogenesis and has antibacterial activity against some Gram-positive bacteria. Pancreastatin inhibits glucose induced insulin release from the pancreas. Chromostatin inhibits catecholamine release from chromaffin cells. Protein type: Secreted; Vesicle; Cell adhesion; Secreted, signal peptide. Chromosomal Location of Human Ortholog: 14q32. Cellular Component: perinuclear region of cytoplasm; extracellular region; secretory granule. Biological Process: regulation of blood pressure

size1 :

0.05 mL

price1 :

240 USD

size2 :

0.1 mL

price2 :

315