14-3-3 epsilon antibody (clone 5A5) | MyBioSource MBS200080 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

MyBioSource

product type :

antibody

product name :

14-3-3 epsilon antibody (clone 5A5)

catalog :

MBS200080

quantity :

0.05 mL

price :

240 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

5A5

reactivity :

human

application :

western blot, ELISA, enzyme immunoassay

product information

catalog number :

MBS200080

products type :

Antibody

products full name :

14-3-3 epsilon antibody (clone 5A5)

products short name :

14-3-3 epsilon

products name syn :

YWHAE; MDS; MDCR; KCIP-1; FLJ45465; 14-3-3 epsilon; Tyrosine 3/tryptophan 5 -monooxygenase activation protein; epsilon polypeptide; Tyrosine 3-monooxygenase/ tryptophan 5-monooxygenase activation protein; epsilon polypeptide 14 3 3 E; 14 3 3E; KCIP 1; KCIP1; Mitochondrial import stimulation factor L subunit; Protein kinase C inhibitor protein1; Tyrosine 3/tryptophan 5 monooxygenase activation protein epsilon polypeptide.

other names :

14-3-3 protein epsilon; 14-3-3 protein epsilon; 14-3-3 protein epsilon; 14-3-3 epsilon; epididymis luminal protein 2; mitochondrial import stimulation factor L subunit; protein kinase C inhibitor protein-1; tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, epsilon polypeptide; tyrosine 3/tryptophan 5 -monooxygenase activation protein, epsilon polypeptide; tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, epsilon

products gene name :

14-3-3 epsilon

other gene names :

YWHAE; YWHAE; MDS; HEL2; MDCR; KCIP-1; 14-3-3E; 14-3-3E

uniprot entry name :

1433E_HUMAN

clonality :

Monoclonal

isotype :

IgG2a, k

clone :

5A5

host :

Mouse

reactivity :

Human

sequence length :

255

purity :

By protein-G affinity chromatography

form :

Liquid. Supplied in Phosphate-Buffered Saline (pH 7.4) with 0.1% Sodium Azide.

concentration :

1mg/ml

storage stability :

Can be stored at 4 degree C. For long term storage, aliquot and store at -20 degree C. Avoid repeated freezing and thawing cycles.

tested application :

ELISA (EIA), Western Blot (WB) (Cell lysate)

app notes :

The antibody has been tested by ELISA and Western blot analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results. Recommended dilution range for Western blot analysis is 1:1,000~ 2,000. Recommended starting dilution is 1:1,000.

other info1 :

Antigen Species: Human. Clone: Anti-human 14-3-3 epsilon mAb, clone 5A5, is derived from hybridization of mouse FO myeloma cells with spleen cells from BALB/c mice immunized with a recombinant human 14-3-3 epsilon protein. Immunogen: Recombinant human 14-3-3 epsilon (1-255aa) purified from E Coli

products categories :

Signal Transduction

products description :

The 14-3-3 family of proteins plays a key regulatory role in signal transduction, checkpoint control, apoptotic and nutrient-sensing pathways. 14-3-3 proteins are highly conserved and ubiquitously expressed. There are at least seven isoforms,beta, gamma,epsilon, sigma, zeta, tau andtau that have been identified in mammals. The 14-3-3 epsilon, a subtype of the 14-3-3 family of proteins, was thought to be brain and neuron-specific. It has been shown to interact with CDC25 phosphatases, RAF1 and IRS1 proteins, suggesting its role in diverse biochemical activities related to signal transduction, such as cell division and regulation of insulin sensitivity. It has also been implicated in the pathogenesis of small cell lung cancer.

products references :

kino T, Pavlakis GN. (2004) DNA Cell Biol. 23(4):193-205 Jin DY, et al., (1996) Nature. 382(6589):308

ncbi gi num :

5803225

ncbi acc num :

NP_006752

ncbi gb acc num :

NM_006761.4

ncbi mol weight :

26,504 Da

ncbi pathways :

Activation Of BAD And Translocation To Mitochondria Pathway (105659); Activation Of BH3-only Proteins Pathway (105658); Alpha6-Beta4 Integrin Signaling Pathway (198807); Anchoring Of The Basal Body To The Plasma Membrane Pathway (1127503); Apoptosis Pathway (105648); Assembly Of The Primary Cilium Pathway (1127502); Calcium Regulation In The Cardiac Cell Pathway (198906); Cell Cycle Pathway (530733); Cell Cycle, Mitotic Pathway (105765); Cell Cycle Pathway (198811)

ncbi summary :

This gene product belongs to the 14-3-3 family of proteins which mediate signal transduction by binding to phosphoserine-containing proteins. This highly conserved protein family is found in both plants and mammals, and this protein is 100% identical to the mouse ortholog. It interacts with CDC25 phosphatases, RAF1 and IRS1 proteins, suggesting its role in diverse biochemical activities related to signal transduction, such as cell division and regulation of insulin sensitivity. It has also been implicated in the pathogenesis of small cell lung cancer. Two transcript variants, one protein-coding and the other non-protein-coding, have been found for this gene. [provided by RefSeq, Aug 2008]

uniprot summary :

14-3-3 epsilon: a protein of the 14-3-3 family of proteins which mediate signal transduction by binding to phosphoserine-containing proteins. A multifunctional regulator of the cell signaling processes. Protein type: Adaptor/scaffold. Chromosomal Location of Human Ortholog: 17p13.3. Cellular Component: kinesin complex; focal adhesion; cytoplasmic vesicle membrane; membrane; mitochondrion; axon; melanosome; cytosol. Molecular Function: protein domain specific binding; protein binding; enzyme binding; potassium channel regulator activity; protein heterodimerization activity; histone deacetylase binding; phosphoprotein binding; phosphoserine binding. Biological Process: substantia nigra development; nerve growth factor receptor signaling pathway; viral reproduction; apoptosis; organelle organization and biogenesis; hippocampus development; neuron migration; cerebral cortex development; mitotic cell cycle; regulation of caspase activity; G2/M transition of mitotic cell cycle; regulation of the rate of heart contraction by hormone; protein targeting. Disease: Miller-dieker Lissencephaly Syndrome

size1 :

0.05 mL

price1 :

240 USD

size2 :

0.1 mL

price2 :

315