Caspase-3 Polyclonal Antibody | MyBioSource MBS194188 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

MyBioSource

product type :

antibody

product name :

Caspase-3 Polyclonal Antibody

catalog :

MBS194188

quantity :

0.05 ml

price :

245 USD

clonality :

polyclonal

host :

rabbit

reactivity :

human

application :

western blot

product information

catalog number :

MBS194188

products type :

Antibody

products full name :

Caspase-3 Polyclonal Antibody

products short name :

Caspase-3

products name syn :

Caspase-3 Antibody; Caspase-3

other names :

caspase-3 preproprotein; Caspase-3; caspase-3; Yama; CASP-3; CPP-32; apopain; procaspase3; protein Yama; OTTHUMP00000165052; OTTHUMP00000165053; OTTHUMP00000165054; OTTHUMP00000165055; OTTHUMP00000224230; OTTHUMP00000224231; PARP cleavage protease; cysteine protease CPP32; SREBP cleavage activity 1; caspase 3, apoptosis-related cysteine protease; caspase 3, apoptosis-related cysteine peptidase; Apopain; Cysteine protease CPP32; CPP-32; Protein Yama; SREBP cleavage activity 1

other gene names :

CASP3; CASP3; CPP32; SCA-1; CPP32B; CPP32

uniprot entry name :

CASP3_HUMAN

clonality :

Polyclonal

host :

Rabbit

specificity :

Caspase-3 Antibody [Rabbit polyclonal antibody to Caspase-3]. This antibody detects a 36 kDa protein on HeLa Cell Lysate SDS-PAGE immunoblots corresponding to the molecular weight of caspase-3. In apoptotic cells this antibody also detects 20 kDa and 18 kDA proteins, corresponding to the processed forms of caspase-3. Upon induction of apoptosis a weak 30kDa band may be detected in some samples. This band is not caspase-3 as it is detected in caspase-3 free MCF7 cell lysates. This antibody reacts with human, mouse, rat, bovine, canine, chicken, guinea pig, hamster, monkey, pig, rabbit, sheep, and yeast caspase-3.

form :

Antiserum

storage stability :

Store frozen product at or below -20 degree C. Thawed product may be stored for 2-4 weeks at 4 degree C. For optimal storage, aliquot and store at -20 degree C.

tested application :

Western Blot

app notes :

Western Blot: 1:1000.

other info1 :

Antigen: Recombinant full length human caspase-3 (His tagged).

products categories :

Apoptosis Antibodies

ncbi gi num :

14790119

ncbi acc num :

NP_004337.2

ncbi gb acc num :

NM_004346.3

uniprot acc num :

P42574

ncbi mol weight :

31,608 Da

ncbi pathways :

Activation Of DNA Fragmentation Factor Pathway 105683!!Activation Of Caspases Through Apoptosome-mediated Cleavage Pathway 105672!!Alpha6-Beta4 Integrin Signaling Pathway 198807!!Alzheimer's Disease Pathway 83097!!Alzheimer's Disease Pathway 509!!Amoebiasis Pathway 167324!!Amoebiasis Pathway 167191!!Amyotrophic Lateral Sclerosis (ALS) Pathway 83099!!Amyotrophic Lateral Sclerosis (ALS) Pathway 511!!Androgen Receptor Signaling Pathway 198806

ncbi summary :

This gene encodes a protein which is a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce two subunits, large and small, that dimerize to form the active enzyme. This protein cleaves and activates caspases 6, 7 and 9, and the protein itself is processed by caspases 8, 9 and 10. It is the predominant caspase involved in the cleavage of amyloid-beta 4A precursor protein, which is associated with neuronal death in Alzheimer's disease. Alternative splicing of this gene results in two transcript variants that encode the same protein. [provided by RefSeq]

uniprot summary :

Function: Involved in the activation cascade of caspases responsible for apoptosis execution. At the onset of apoptosis it proteolytically cleaves poly(ADP-ribose) polymerase (PARP) at a '216-Asp- -Gly-217' bond. Cleaves and activates sterol regulatory element binding proteins (SREBPs) between the basic helix-loop-helix leucine zipper domain and the membrane attachment domain. Cleaves and activates caspase-6, -7 and -9. Involved in the cleavage of huntingtin. Ref.8. Catalytic activity: Strict requirement for an Asp residue at positions P1 and P4. It has a preferred cleavage sequence of Asp-Xaa-Xaa-Asp- - with a hydrophobic amino-acid residue at P2 and a hydrophilic amino-acid residue at P3, although Val or Ala are also accepted at this position. Enzyme regulation: Inhibited by isatin sulfonamides. Subunit structure: Heterotetramer that consists of two anti-parallel arranged heterodimers, each one formed by a 17 kDa (p17) and a 12 kDa (p12) subunit. Subcellular location: Cytoplasm. Tissue specificity: Highly expressed in lung, spleen, heart, liver and kidney. Moderate levels in brain and skeletal muscle, and low in testis. Also found in many cell lines, highest expression in cells of the immune system. Post-translational modification: Cleavage by granzyme B, caspase-6, caspase-8 and caspase-10 generates the two active subunits. Additional processing of the propeptides is likely due to the autocatalytic activity of the activated protease. Active heterodimers between the small subunit of caspase-7 protease and the large subunit of caspase-3 also occur and vice versa.S-nitrosylated on its catalytic site cysteine in unstimulated human cell lines and denitrosylated upon activation of the Fas apoptotic pathway, associated with an increase in intracellular caspase activity. Fas therefore activates caspase-3 not only by inducing the cleavage of the caspase zymogen to its active subunits, but also by stimulating the denitrosylation of its active site thiol. Sequence similarities: Belongs to the peptidase C14A family.

size :

0.05 ml

price :

245 USD