Human Epidermal Growth Factor ReceptorMonoclonal Antibody | MyBioSource MBS190230 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

MyBioSource

product type :

antibody

product name :

Human Epidermal Growth Factor ReceptorMonoclonal Antibody

catalog :

MBS190230

quantity :

0.1 mg

price :

390 USD

clonality :

monoclonal

host :

mouse

reactivity :

human

application :

western blot, immunohistochemistry, flow cytometry

product information

catalog number :

MBS190230

products type :

Antibody

products full name :

Human Epidermal Growth Factor ReceptorMonoclonal Antibody

products short name :

Human Epidermal Growth Factor Receptor

products name syn :

Human Epidermal Growth Factor Receptor; Epidermal Growth Factor Receptor

other names :

epidermal growth factor receptor; Epidermal growth factor receptor; epidermal growth factor receptor; OTTHUMP00000159661; OTTHUMP00000159662; OTTHUMP00000159663; OTTHUMP00000209210; OTTHUMP00000209211; proto-oncogene c-ErbB-1; cell growth inhibiting protein 40; cell proliferation-inducing protein 61; receptor tyrosine-protein kinase erbB-1; avian erythroblastic leukemia viral (v-erb-b) oncogene homolog; epidermal growth factor receptor; Proto-oncogene c-ErbB-1; Receptor tyrosine-protein kinase erbB-1

other gene names :

EGFR; EGFR; ERBB; HER1; mENA; ERBB1; PIG61; ERBB1

uniprot entry name :

EGFR_HUMAN

clonality :

Monoclonal

isotype :

IgG2a

clone :

B4G7

host :

Mouse

sequence length :

1210

specificity :

Human Epidermal Growth Factor Receptor [Mouse monoclonal antibody to human Epidermal Growth Factor Receptor]. This antibody specifically reacts with the human EGF receptor and inhibits binding of EGF to the receptor by recognizing the EGF binding site on the receptor. This antibody does not react with rat EGF receptor.

form :

100 ug IgG in 10 mM PBS, pH 7.4, lyophilized.

storage stability :

Lyophilized antibody is stable at 4 degree C for 2 years. Store the reconstituted solution in aliquots at -20 degree C for one year or at 4 degree C for 6 months. Avoid repeated freeze-thaw cycles.

tested application :

Western Blot, Flow Cytometry, Immunohistochemistry

app notes :

Western Blotting: 5-10 ug/ml. Immunohistochemistry: 5-10 ug/ml. Flow Cytometry: 1 ug/ml

other info1 :

Antigen: Human epidermal carcinoma cell line A431.

other info2 :

Stabilizer: 1% BSA. Preservatives: 0.1% Sodium Azide. Dilutions Instructions: Reconstitute in distilled water to the final desired concentration.

products categories :

Growth Factors/Cytokines/Receptor Antibodies

ncbi gi num :

553271

ncbi acc num :

AAA63171.1

uniprot acc num :

P00533

ncbi mol weight :

134,277 Da

ncbi pathways :

Adherens Junction Pathway 83070!!Adherens Junction Pathway 481!!Alpha6-Beta4 Integrin Signaling Pathway 198807!!Androgen Receptor Signaling Pathway 198806!!Arf6 Signaling Events Pathway 138034!!Axon Guidance Pathway 105688!!Bladder Cancer Pathway 83115!!Bladder Cancer Pathway 527!!Calcium Signaling Pathway 83050!!Calcium Signaling Pathway 459

ncbi summary :

The protein encoded by this gene is a transmembrane glycoprotein that is a member of the protein kinase superfamily. This protein is a receptor for members of the epidermal growth factor family. EGFR is a cell surface protein that binds to epidermal growth factor. Binding of the protein to a ligand induces receptor dimerization and tyrosine autophosphorylation and leads to cell proliferation. Mutations in this gene are associated with lung cancer. Multiple alternatively spliced transcript variants that encode different protein isoforms have been found for this gene. [provided by RefSeq]

uniprot summary :

Function: Cell surface receptor for EGF, but also for other members of the EGF family, such as TGF-alpha, BTC/betacellulin, AREGAREGB/amphiregulin, HBEGF, GP30 and vaccinia virus growth factor. Ligand binding triggers a conformation change, leading to activation of the kinase and subsequent phosphorylation of down-stream protein kinases. Is involved in the control of cell growth, proliferation and differentiation. Phosphorylates MUC1 in breast cancer cells and increases the interaction of MUC1 with SRC and CTNNB1/beta-catenin. Ref.33 Ref.54 Ref.55 Ref.56 Ref.60 Ref.63 Ref.65Isoform 2 may act as an antagonist of EGF action. Ref.33 Ref.54 Ref.55 Ref.56 Ref.60 Ref.63 Ref.65. Catalytic activity: ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate. Ref.56 Ref.59 Ref.60 Ref.61 Ref.62 Ref.63. Subunit structure: EGF binding triggers dimerization of the receptor and promotes autophosphorylation. The activate receptor dimer binds one EGF molecule. Heterodimer with ERBB2. Interacts with ERRFI1. Interaction with ERRFI1 inhibits dimerization of the kinase domain and autophosphorylation. Binds RIPK1. Interacts (via autophosphorylated C-terminal tail) with CBL. Part of a complex with ERBB2 and either PIK3C2A or PIK3C2B. Interacts (autophosphorylated form) with PIK3C2B; the interaction may be indirect. Interacts with PELP1. Binds MUC1. Interacts with AP2M1. Interacts with GAB2. Interacts with COPG, upon EGF treatment; interaction is essential for regulation of EGF-dependent nuclear transport of EGFR by retrograde trafficking from the Golgi to the ER. Interacts with TNK2; this interaction is dependent on EGF stimulation and kinase activity of EGFR. Ref.26 Ref.28 Ref.31 Ref.32 Ref.33 Ref.37 Ref.41 Ref.44 Ref.52 Ref.54 Ref.55 Ref.60 Ref.62 Ref.63 Ref.64 Ref.65. Subcellular location: Cell membrane; Single-pass type I membrane protein. Endoplasmic reticulum membrane; Single-pass type I membrane protein. Golgi apparatus membrane; Single-pass type I membrane protein. Nucleus membrane; Single-pass type I membrane protein. Endosome. Note: In response to EGF, translocated from the cell membrane to the nucleus via Golgi and ER. Co-localizes with TNK2 on the endosomes. Ref.41 Ref.52 Ref.54 Ref.63Isoform 2: Secreted Ref.41 Ref.52 Ref.54 Ref.63. Tissue specificity: Ubiquitously expressed. Isoform 2 is also expressed in ovarian cancers. Ref.40. Post-translational modification: Phosphorylation of Ser-695 is partial and occurs only if Thr-693 is phosphorylated. Ref.17 Ref.25 Ref.30 Ref.34 Ref.35 Ref.36 Ref.38 Ref.39 Ref.42 Ref.43 Ref.45 Ref.46 Ref.47 Ref.48 Ref.50 Ref.51 Ref.59 Ref.60 Ref.62 Ref.63Monoubiquitinated and polyubiquitinated upon EGF stimulation; which does not affect tyrosine kinase activity or signaling capacity but may play a role in lysosomal targeting. Polyubiquitin linkage is mainly through 'Lys-63', but linkage through 'Lys-48', 'Lys-11' and 'Lys-29' also occur. Involvement in disease: Defects in EGFR are associated with lung cancer (LNCR) [. MIM:211980]. Miscellaneous: Binding of EGF to the receptor leads to dimerization, internalization of the EGF-receptor complex, induction of the tyrosine kinase activity, stimulation of cell DNA synthesis, and cell proliferation. Sequence similarities: Belongs to the protein kinase superfamily. Tyr protein kinase family. EGF receptor subfamily.Contains 1 protein kinase domain.

size :

0.1 mg

price :

390 USD