Estrogen Receptor- alpha Monoclonal Antibody
Estrogen Receptor Alpha
Anti-Estrogen Receptor Alpha; Estrogen Receptor-alpha
estrogen receptor alpha; Putative uncharacterized protein; estrogen receptor; ER-alpha; OTTHUMP00000017718; OTTHUMP00000017719; estradiol receptor; estrogen receptor alpha delta 4 +49 isoform; nuclear receptor subfamily 3 group A member 1; estrogen receptor alpha delta 4*,5,6,7*/654 isoform; estrogen receptor alpha delta 3*,4,5,6,7*/819-2 isoform; estrogen receptor 1;
ESR1; ER; ESR; Era; ESRA; NR3A1; DKFZp686N23123;
Anti-Estrogen Receptor Alpha [Murine MAb to fusion protein corresponding to aa 1-190 of human estrogen receptor-alpha, a member of the steroid family of nuclear receptors.]. This antibody recognizes human ER- alpha , a 65 kD protein and a member of the steroid family of nuclear receptors. ER- alpha is a ligand-activated transcription factor that, when bound to estrogen, induces a conformational change that allows dimerization and binding to estrogen response element sequences. When bound to DNA, ER- alpha can positively or negatively regulate gene transcription.
100 ug Protein G-purified antibody in PBS, pH 7.4
This antibody is stable for at least one (1) year at -70 degree C. Avoid multiple freeze thaw cycles.
Immunoblotting: use at 1-5 ug/ml. Immunoprecipitation: use at 1-5 ug/ml. Positive controls: MCF-7 cells andrecombinant protein.
Antigen: 6-His fusion proteincontaining the region encoding aa 1-190of human estrogen receptor- alpha (ER- alpha ) expressed in E. coli.
Stabilizer None!!Preservatives None. Available on request.!!Dilutions Instructions Dilute in PBS or medium which is identical to that used in the assay system.
Cancer Research Products
Androgen Receptor Signaling Pathway 198806!!Endocrine And Other Factor-regulated Calcium Reabsorption Pathway 213307!!Endocrine And Other Factor-regulated Calcium Reabsorption Pathway 213276!!Estrogen Signaling Pathway 198863!!FOXA1 Transcription Factor Network Pathway 137979!!FOXM1 Transcription Factor Network Pathway 137935!!Gene Expression Pathway 105937!!Generic Transcription Pathway 105938!!LKB1 Signaling Events Pathway 137957!!Nuclear Receptor Transcription Pathway 105979
This gene encodes an estrogen receptor, a ligand-activated transcription factor composed of several domains important for hormone binding, DNA binding, and activation of transcription. The protein localizes to the nucleus where it may form a homodimer or a heterodimer with estrogen receptor 2. Estrogen and its receptors are essential for sexual development and reproductive function, but also play a role in other tissues such as bone. Estrogen receptors are also involved in pathological processes including breast cancer, endometrial cancer, and osteoporosis. Alternative splicing results in several transcript variants, which differ in their 5' UTRs and use different promoters. [provided by RefSeq]
1. GWAS for discovery and replication of genetic loci associated with sudden cardiac arrest in patients with coronary artery disease. . 2. A genome-wide association study identifies novel loci associated with susceptibility to chronic myeloid leukemia. . 3. Replication study of the association between adolescent idiopathic scoliosis and two estrogen receptor genes. . 4. Overcoming resistance to fulvestrant (ICI182,780) by downregulating the c-ABL proto-oncogene in breast cancer. . 5. Estrogen receptor Î± induces down-regulation of PTEN through PI3-kinase activation in breast cancer cells.
Human oestrogen receptor cDNA: sequence, expression and homology to v-erb-A. Green S., Walter P., Kumar V., Krust A., Bornert J.-M., Argos P., Chambon P.Nature 320:134-139(1986) [PubMed: 3754034] [Abstract]