MyBioSource

ELISA/assay

Human Leptin receptor PicoKine ELISA Kit

MBS175913

1 x96 Wells

380 USD

MBS175913

ELISA Kit

Human Leptin receptor PicoKine ELISA Kit

Leptin receptor

Leptin receptor; leptin receptor; CD295; HuB219; LEP-R; LEPR; LEPR_HUMAN; Leptin receptor; OB receptor; OB-R; OBR

Leptin receptor; Leptin receptor; leptin receptor; huB219; OB receptor; leptin receptor; HuB219; OB receptor; OB-R; CD_antigen: CD295

LEPR

LEPR; LEPR; OBR; OB-R; CD295; LEP-R; LEPRD; DB; OBR; LEP-R; OB-R

LEPR_HUMAN

Human

1165

Natural and recombinant human sLeptin receptor

Store at 4 degree C for 6 months, at -20 degree C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

Samples: For quantitative detection of human Leptin receptor in cell culture supernates, serum and plasma(heparin, EDTA). Detection Range: 156pg/ml-10,000pg/ml

Sensitivity: < 8pg/ml

Principle of the assay: human Leptin receptor ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for Leptin receptor has been precoated onto 96-well plates. Standards(NSO,T20-D839) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for Leptin receptor is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human Leptin receptor amount of sample captured in plate. Background: Leptin receptor (or Obese receptor, OBR) is a single membrane-spanning receptor most related to the gp130 signal-transducing component of the IL-6 receptor, the G-CSF receptor, and the LIF receptor. OB-R mRNA is expressed not only in choroid plexus, but also in several other tissues, including hypothalamus. Leptin acts through the leptin receptor, a single-transmembrane domain receptor of the cytokine-receptor family. Leptin controls energy balance and food intake through the leptin receptor in the hypothalamus of the brain, which suggests that some polymorphisms of the leptin receptor gene (LEPR) might contribute to obesity or obesity-related diseases. Leptin is also involved in the regulation of blood pressure through the leptin receptor.

116242617

P48357.2

P48357

96,042 Da

AMPK Signaling Pathway 198868!!AMPK Signaling Pathway 989139!!AMPK Signaling Pathway 992181!!Adipocytokine Signaling Pathway 83093!!Adipocytokine Signaling Pathway 505!!Cytokine-cytokine Receptor Interaction Pathway 83051!!Cytokine-cytokine Receptor Interaction Pathway 460!!Jak-STAT Signaling Pathway 83077!!Jak-STAT Signaling Pathway 488!!Leptin Signaling Pathway 672456

The protein encoded by this gene belongs to the gp130 family of cytokine receptors that are known to stimulate gene transcription via activation of cytosolic STAT proteins. This protein is a receptor for leptin (an adipocyte-specific hormone that regulates body weight), and is involved in the regulation of fat metabolism, as well as in a novel hematopoietic pathway that is required for normal lymphopoiesis. Mutations in this gene have been associated with obesity and pituitary dysfunction. Alternatively spliced transcript variants encoding different isoforms have been described for this gene. It is noteworthy that this gene and LEPROT gene (GeneID:54741) share the same promoter and the first 2 exons, however, encode distinct proteins (PMID:9207021).[provided by RefSeq, Nov 2010]

1 x96 Wells

380 USD