ELISA/assay

Human P53 PicoKine ELISA Kit

MBS175896

1 x96 Wells

475 USD

ELISA Kit

Human P53 PicoKine ELISA Kit

P53

Cellular tumor antigen p53; tumor protein p53; Antigen NY-CO-13; BCC7; Cellular tumor antigen p53; LFS1; MS980; p53; p53 tumor suppressor; P53_HUMAN; Phosphoprotein p53; Tp53; Transformation related protein 53; TRP53; Tumor suppressor p53

Cellular tumor antigen p53; cellular tumor antigen p53; antigen NY-CO-13; tumor protein 53; phosphoprotein p53; p53 tumor suppressor; mutant tumor protein 53; transformation-related protein 53; tumor protein p53

TP53

TP53; TP53; P53; BCC7; LFS1; TRP53

K7PPA8_HUMAN

Human

393

Natural and recombinant human P53

Store at 4 degree C for 6 months, at -20 degree C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

Samples: For quantitative detection of human P53 in cell lysates. Detection Range: 156pg/ml-10,000pg/ml

Sensitivity: < 10pg/ml. Intra-assay Precision: Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision. Inter-assay Precision: Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.

Principle of the assay: human P53 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for P53 has been precoated onto 96-well plates. Standards(NSO, M1-D393) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for P53 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human P53 amount of sample captured in plate. Background: P53 (also known as protein 53 or tumor protein 53), is a tumor suppressor protein that in humans is encoded by the TP53 gene. The human P53 gene is mapped to chromosome 17. Human p53 is 393 amino acids long and has seven domains. It runs as a 53-kilodalton (kDa) protein on SDS-PAGE. The pattern of p53 splicing was specific for brain areas and for individuals. And human kidney and heart expressed only full-length p53. It has played a vital role in conserving stability by preventing genome mutation.

K7PPA8

43,653 Da

AMPK Signaling Pathway 198868!!Activation Of BH3-only Proteins Pathway 105658!!Activation Of NOXA And Translocation To Mitochondria Pathway 105660!!Activation Of PUMA And Translocation To Mitochondria Pathway 105661!!Alzheimers Disease Pathway 672448!!Amyotrophic Lateral Sclerosis (ALS) Pathway 920975!!Amyotrophic Lateral Sclerosis (ALS) Pathway 83099!!Amyotrophic Lateral Sclerosis (ALS) Pathway 511!!Apoptosis Pathway 198797!!Apoptosis Pathway 83060

This gene encodes a tumor suppressor protein containing transcriptional activation, DNA binding, and oligomerization domains. The encoded protein responds to diverse cellular stresses to regulate expression of target genes, thereby inducing cell cycle arrest, apoptosis, senescence, DNA repair, or changes in metabolism. Mutations in this gene are associated with a variety of human cancers, including hereditary cancers such as Li-Fraumeni syndrome. Alternative splicing of this gene and the use of alternate promoters result in multiple transcript variants and isoforms. Additional isoforms have also been shown to result from the use of alternate translation initiation codons (PMIDs: 12032546, 20937277). [provided by RefSeq, Feb 2013]

1 x96 Wells

475 USD