product summary
request information :
company name :
MyBioSource
product type :
protein
product name :
Recombinant Porcine Tumor Necrosis Factor-alpha
catalog :
MBS142281
quantity :
0.02 mg
price :
205 USD
more info or order :
MyBioSource product webpage
product information
catalog number :
MBS142281
products type :
Recombinant Protein
products full name :
Recombinant Porcine Tumor Necrosis Factor-alpha
products short name :
Tumor Necrosis Factor-alpha
products name syn :
TNF a Porcine; Tumor Necrosis Factor-Alpha Porcine Recombinant; TNF-alpha; Tumor necrosis factor ligand superfamily member 2; TNF-a; Cachectin; DIF; TNFA; TNFSF2
other names :
tumor necrosis factor; Tumor necrosis factor; tumor necrosis factor; TNF-a; TNF-alpha; cachectin; tumor necrosis factor (TNF superfamily, member 2); tumor necrosis factor alpha; tumor necrosis factor ligand superfamily member 2; tumor-necrosis factor; Cachectin; TNF-alpha; Tumor necrosis factor ligand superfamily member 2; TNF-a
products gene name :
rpTNF a
other gene names :
TNF; TNF; TNFa; TNFA; TNFSF2; TNF-a; NTF; ICD1; ICD2
uniprot entry name :
TNFA_PIG
host :
E Coli
sequence length :
232
sequence :
The sequence of the first five N-terminal amino acids was determined and was found to be Met-Leu-Arg-Ser-Ser.
purity :
Greater than 95.0% as determined by: (a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE.
form :
The protein was lyophilized with no additives. Sterile Filtered White lyophilized (freeze-dried) powder.
storage stability :
Lyophilized Tumor Necrosis Factor-a although stable at room temperature for 3 weeks, should be stored desiccated below -18 degree C. Upon reconstitution TNF-a should be stored at 4 degree C between 2-7 days and for future use below -18 degree C.For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please prevent freeze-thaw cycles.
other info2 :
Solubility: It is recommended to reconstitute the lyophilized Tumor Necrosis Factor-alpha in sterile 18M Omega -cm H2O not less than 100 ug/ml, which can then be further diluted to other aqueous solutions. Protein Content: Protein quantitation was carried out by two independent methods:1. UV spectroscopy at 280 nm using the absorbency value of 1.26 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics). 2. Analysis by RP-HPLC, using a calibrated solution of TNF-a as a Reference Standard. Biological Activity: The ED50 as determined by the cytotoxicity of porcine PK15 cells was found to be 0.001-0.015 ng/ml.
products categories :
CYTOKINES AND GROWTH FACTORS; Cytokines; Tumor Necrosis Factor
products description :
Description: Tumor Necrosis Factor-a Porcine Recombinant produced in E Coli is a single, non-glycosylated, polypeptide chain containing 157 amino acids and having a molecular mass of 17,274 Dalton. The TNF-alpha is purified by standard chromatographic techniques. Introduction: Tumor necrosis factor is a cytokine involved in systemic inflammation and is a member of a group of cytokines that all stimulate the acute phase reaction. TNF is mainly secreted by macrophages. TNF causes apoptotic cell death, cellular proliferation, differentiation, inflammation, tumorigenesis and viral replication, TNF is also involved in lipid metabolism, and coagulation. TNF's primary role is in the regulation of immune cells.Dysregulation and, in particular, overproduction of TNF have been implicated in a variety of human diseases- autoimmune diseases, insulin resistance, and cancer.
ncbi gi num :
47522866
ncbi acc num :
NP_999187.1
ncbi gb acc num :
NM_214022.1
uniprot acc num :
P23563
ncbi mol weight :
25,254 Da
ncbi pathways :
Adipocytokine Signaling Pathway 84504!!Adipocytokine Signaling Pathway 505!!African Trypanosomiasis Pathway 194379!!African Trypanosomiasis Pathway 194323!!Allograft Rejection Pathway 84530!!Allograft Rejection Pathway 535!!Alzheimer's Disease Pathway 84508!!Alzheimer's Disease Pathway 509!!Amoebiasis Pathway 167313!!Amoebiasis Pathway 167191
size :
0.02 mg
price :
205 USD
more info or order :
MyBioSource product webpage
company information
MyBioSource
P.O. Box 153308
San Diego, CA 92195-3308
sales@mybiosource.com
www.mybiosource.com
1-888-627-0165
headquarters: USA
MyBioSource, LLC was orginally founded in Vancouver by three enthusiastic scientists who are passionate about providing the world with the best reagents available. Together, they form a company with a big vision known as MyBioSource. MyBioSource is now located in San Diego, California, USA.

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