product summary
request information :
company name :
MyBioSource
product type :
protein
product name :
Recombinant Mouse Leptin
catalog :
MBS142228
quantity :
0.2 mg
price :
140 USD
more info or order :
MyBioSource product webpage
product information
catalog number :
MBS142228
products type :
Recombinant Protein
products full name :
Recombinant Mouse Leptin
products short name :
Leptin
products name syn :
Leptin Mouse; Leptin Mouse Recombinant; OB Protein; Obesity Protein; OBS; Obesity factor; mLeptin
other names :
leptin; Leptin; leptin; obesity factor; leptin; Obesity factor
other gene names :
Lep; Lep; ob; obese; Ob
uniprot entry name :
LEP_MOUSE
host :
E Coli
sequence length :
167
sequence :
MVPIQKVQDD TKTLIKTIVT RINDISHTQS VSAKQRVTGL DFIPGLHPIL SLSKMDQTLA VYQQVLTSLP SQNVLQIAND LENLRDLLHL LAFSKSCSLP QTSGLQKPES LDGVLEASLY STEVVALSRL QGSLQDILQQ LDVSPEC.
purity :
Greater than 95.0% as determined by: (a) Analysis by SEC-HPLC. (b) Analysis by SDS-PAGE.
form :
The mouse Leptin was lyophilized from a concentrated (1mg/ml) solution containing 0.1% TFA. Sterile Filtered White lyophilized (freeze-dried) powder.
storage stability :
Lyophilized Leptin although stable at room temperature for 3 weeks, should be stored desiccated below -18 degree C. Upon reconstitution Leptin should be stored at 4 degree C between 2-7 days and for future use below -18 degree C.For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please prevent freeze-thaw cycles.
other info2 :
Solubility: It is recommended to reconstitute the lyophilized Leptin in sterile 18M Omega -cm H2O not less than 100 ug/ml, which can then be further diluted to other aqueous solutions. Protein Content: Protein quantitation was carried out by two independent methods: 1. UV spectroscopy at 280 nm using the absorbency value of 0.201 as the extinctioncoefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics). 2. Analysis by RP-HPLC, using a calibrated solution of Leptin as a Reference Standard. Biological Activity: Biological activity of Mouse Leptin is performed in two different mouse obesity models, ob/ob and NZO. Both strains of mice were treated via intraperitoneal injection once daily at a dose of 5ug Leptin/gram body weight for a period of 14 days. Significant effects on body weight, food consumption, and plasma glucose levels were observed to saline-treated controls.
products categories :
CYTOKINES AND GROWTH FACTORS; Growth Factors; Leptin
products description :
Description: Leptin Mouse Recombinant produced in E Coli is a single, non-glycosylated, polypeptide chain containing 147 amino acids and having a molecular mass of 16.2 kDa.The Leptin is purified by proprietary chromatographic techniques. Introduction: A 16-kDa peptide hormone secreted from white adipocytes and implicated in the regulation of food intake and energy balance. Leptin provides the key afferent signal from fat cells in the feedback system that controls body fat stores.
ncbi gi num :
6678678
ncbi acc num :
NP_032519.1
ncbi gb acc num :
NM_008493.3
uniprot acc num :
P41160
ncbi mol weight :
18,709 Da
ncbi pathways :
AMPK Signaling Pathway 989651!!AMPK Signaling Pathway 992181!!Adipocytokine Signaling Pathway 83290!!Adipocytokine Signaling Pathway 505!!Adipogenesis Pathway 198299!!Cytokine-cytokine Receptor Interaction Pathway 83248!!Cytokine-cytokine Receptor Interaction Pathway 460!!Incretin Synthesis, Secretion, And Inactivation Pathway 1110259!!Jak-STAT Signaling Pathway 83274!!Jak-STAT Signaling Pathway 488
size :
0.2 mg
price :
140 USD
more info or order :
MyBioSource product webpage
company information
MyBioSource
P.O. Box 153308
San Diego, CA 92195-3308
sales@mybiosource.com
www.mybiosource.com
1-888-627-0165
headquarters: USA
MyBioSource, LLC was orginally founded in Vancouver by three enthusiastic scientists who are passionate about providing the world with the best reagents available. Together, they form a company with a big vision known as MyBioSource. MyBioSource is now located in San Diego, California, USA.

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