Recombinant Human Hepatocyte Growth Factor, CHO | MyBioSource MBS142128 product information

product summary

request information :

company name :

MyBioSource

product type :

protein

product name :

Recombinant Human Hepatocyte Growth Factor, CHO

catalog :

MBS142128

quantity :

0.01 mg

price :

205 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS142128

products type :

Recombinant Protein

products full name :

Recombinant Human Hepatocyte Growth Factor, CHO

products short name :

Hepatocyte Growth Factor

products name syn :

HGF Human CHO; Hepatocyte Growth Factor Human Recombinant CHO; Scatter Factor (SF); Hepatopoietin (HPTA); HGF; HGF CHO

other names :

hepatocyte growth factor isoform 1 preproprotein; Hepatocyte growth factor; hepatocyte growth factor; fibroblast-derived tumor cytotoxic factor; hepatopoeitin-A; hepatopoietin-A; lung fibroblast-derived mitogen; hepatocyte growth factor (hepapoietin A; scatter factor); Hepatopoietin-A; Scatter factor; SF

products gene name :

HGF

other gene names :

HGF; HGF; SF; HGFB; HPTA; F-TCF; DFNB39; HPTA; SF

uniprot entry name :

HGF_HUMAN

host :

Chinese Hamster Ovarian Cells (CHO)

sequence length :

728

sequence :

Agrees with the sequence of native human HGF.

purity :

Greater than 97.0% as determined by: (a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE.

form :

The protein was lyophilized from a concentrated solution containing Phosphate-Buffered Saline with 0.02% Tween 80, pH 7.4. Sterile Filtered White lyophilized (freeze-dried) powder.

storage stability :

Lyophilized Hepatocyte Growth Factor although stable at room temperature for 3 weeks, should be stored desiccated below -18 degree C. Upon reconstitution HGF should be stored at 4 degree C between 2-7 days and for future use below -18 degree C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please prevent freeze-thaw cycles.

other info2 :

Solubility: It is recommended to reconstitute the lyophilized Hepatocyte Growth Factor in sterile 18M-cm H2O not less than 100 ug/ml, which can then be further diluted to other aqueous solutions. Protein Content: Protein quantitation was carried out by two independent methods:1. UV spectroscopy at 280 nm using the absorbency value of 1.83 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics). 2. Analysis by RP-HPLC, using a calibrated solution of HGF as a Reference Standard. Biological Activity: The ED50, calculated by the dose-dependant proliferation of monkey 4MBr-5 indicator cells was found to be 20-40ng/ml corresponding to a Specific Activity of 25,000-50,000IU/mg.

products categories :

CYTOKINES AND GROWTH FACTORS; Growth Factors; Hepatocyte Growth Factor

products description :

Description: Hepatocyte Growth Factor Human Recombinant produced in CHO is a heterodimer, non-glycosylated, polypeptide chain consisting an a-chain of 463 amino acids and b-chain of 234 having a total molecular mass of 75kDa.The HGF is purified by proprietary chromatographic techniques. Introduction: Hepatocyte Growth Factor (HGF) is a multifunctional growth factor which regulates both cell growth and cell motility. It exerts a strong mitogenic effect on hepatocytes and primary epithelial cells. HGF synergizes with Interleukin-3and GM-CSFto stimulate colony formation of hematopoietic progenitor cells in vitro and may, therefore, also modulate hematopoiesis.

ncbi gi num :

33859835

ncbi acc num :

NP_000592.3

ncbi gb acc num :

NM_000601.4

uniprot acc num :

P14210

ncbi mol weight :

24,116 Da

ncbi pathways :

Arf6 Signaling Events Pathway 138034!!Cytokine Signaling In Immune System Pathway 366171!!Cytokine-cytokine Receptor Interaction Pathway 83051!!Cytokine-cytokine Receptor Interaction Pathway 460!!Direct P53 Effectors Pathway 137939!!FGF Signaling Pathway 137989!!Focal Adhesion Pathway 198795!!Focal Adhesion Pathway 83067!!Focal Adhesion Pathway 478!!Hemostasis Pathway 106028

ncbi summary :

Hepatocyte growth factor regulates cell growth, cell motility, and morphogenesis by activating a tyrosine kinase signaling cascade after binding to the proto-oncogenic c-Met receptor. Hepatocyte growth factor is secreted by mesenchymal cells and acts as a multi-functional cytokine on cells of mainly epithelial origin. Its ability to stimulate mitogenesis, cell motility, and matrix invasion gives it a central role in angiogenesis, tumorogenesis, and tissue regeneration. It is secreted as a single inactive polypeptide and is cleaved by serine proteases into a 69-kDa alpha-chain and 34-kDa beta-chain. A disulfide bond between the alpha and beta chains produces the active, heterodimeric molecule. The protein belongs to the plasminogen subfamily of S1 peptidases but has no detectable protease activity. Alternative splicing of this gene produces multiple transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008]

size :

0.01 mg

price :

205 USD

more info or order :

MyBioSource product webpage