Mouse Anti-Vimentin Monoclonal Antibody | MyBioSource MBS130158 product information

product summary

request information :

company name :

MyBioSource

product type :

antibody

product name :

Mouse Anti-Vimentin Monoclonal Antibody

catalog :

MBS130158

quantity :

0.04 mg

price :

150 USD

clonality :

monoclonal

host :

mouse

reactivity :

human

application :

ELISA

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS130158

products type :

Antibody

products full name :

Mouse Anti-Vimentin Monoclonal Antibody

products short name :

Vimentin

other names :

vimentin; Vimentin; vimentin; OTTHUMP00000019224; OTTHUMP00000019225; vimentin; N/A

products gene name :

Vimentin

other gene names :

VIM; VIM; FLJ36605; N/A

uniprot entry name :

VIME_HUMAN

clonality :

Monoclonal

isotype :

IgG1

host :

Mouse

sequence length :

466

specificity :

Vimentin recombinant protein (WB). human Vimentin recombinant protein (ELISA). human lung carcinoma tissue (IHC)

purity :

Mouse Anti-Vimentin is purified by protein A affinity chromatography

concentration :

1 mg/ml in 10 mM PBS (pH 7.6) and 0.02% sodium azide

storage stability :

For long-term storage. aliquot and store at -20 degree C or below. Use before is one year from the date of receipt.

tested application :

ELISA

app notes :

ELISA: Propose dilution 1:10,000. Determining optimal working dilutions by titration test

other info1 :

Immunogen: Ni-NTA purified truncated recombinant Vimentin expressed in E. coli strain BL21 (DE3). Antigen Species: Human

products categories :

Cancer/Apoptosis Pathway

products description :

Vimentin is the major subunit protein of the intermediate filaments of mesenchymal cells. It is believed to be involved with the intracellular transport of proteins between the nucleus and plasma membrane. Vimentin has been implicated to be involved in the rate of steroid synthesis via its role as a storage network for steroidogenic cholesterol containing lipid droplets. Vimentin phosphorylation by a protein kinase causes the breakdown of intermediate filaments and activation of an ATP and myosin light chain dependent contractile event. This results in cytoskeletal changes that facilitate the interaction of the lipid droplets within mitochondria. and subsequent transport of cholesterol to the organelles leading to an increase in steroid synthesis. Immunohistochemical staining for Vimentin is characteristic of sarcomas (of neural. muscle and fibroblast origin) compared to carcinomas which are generally negative. Melanomas. lymphomas and vascular tumors may all stain for Vimentin. Vimentin antibodies are thus of value in the differential diagnosis of undifferentiated neoplasms and malignant tumors. They are generally used with a panel of other antibodies including those recognising cytokeratins. lymphoid markers. S100. desmin and neurofilaments.

ncbi gi num :

62414289

ncbi acc num :

NP_003371.2

ncbi gb acc num :

NM_003380.3

uniprot acc num :

P08670

ncbi mol weight :

53,652 Da

ncbi pathways :

Alpha6-Beta4 Integrin Signaling Pathway 198807!!Apoptosis Pathway 105648!!Apoptotic Cleavage Of Cellular Proteins Pathway 105678!!Apoptotic Execution Phase Pathway 105677!!Aurora B Signaling Pathway 138080!!Caspase Cascade In Apoptosis Pathway 137974!!Caspase-mediated Cleavage Of Cytoskeletal Proteins Pathway 105679!!Muscle Contraction Pathway 106261!!Striated Muscle Contraction Pathway 106262!!Striated Muscle Contraction Pathway 198903

ncbi summary :

This gene encodes a member of the intermediate filament family. Intermediate filamentents, along with microtubules and actin microfilaments, make up the cytoskeleton. The protein encoded by this gene is responsible for maintaining cell shape, integrity of the cytoplasm, and stabilizing cytoskeletal interactions. It is also involved in the immune response, and controls the transport of low-density lipoprotein (LDL)-derived cholesterol from a lysosome to the site of esterification. It functions as an organizer of a number of critical proteins involved in attachment, migration, and cell signaling. Mutations in this gene causes a dominant, pulverulent cataract.

uniprot summary :

Function: Vimentins are class-III intermediate filaments found in various non-epithelial cells, especially mesenchymal cells. Subunit structure: Homopolymer. Interacts with HCV core protein. Interacts with LGSN and SYNM. Interacts (via rod region) with PLEC (via CH 1 domain). By similarity. Interacts with SLC6A4. Ref.22 Ref.35. Tissue specificity: Highly expressed in fibroblasts, some expression in T- and B-lymphocytes, and little or no expression in Burkitt's lymphoma cell lines. Expressed in many hormone-independent mammary carcinoma cell lines. Ref.12 Ref.17. Post-translational modification: Filament disassembly during mitosis is promoted by phosphorylation at Ser-55 as well as by nestin. By similarity. One of the most prominent phosphoproteins in various cells of mesenchymal origin. Phosphorylation is enhanced during cell division, at which time vimentin filaments are significantly reorganized. Phosphorylation by PKN1 inhibits the formation of filaments. Sequence similarities: Belongs to the intermediate filament family. Sequence caution: The sequence BAB71275.1 differs from that shown. Reason: Intron retention.

size :

0.04 mg

price :

150 USD

more info or order :

MyBioSource product webpage