This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
LifeSpan Biosciences
product type :
antibody
product name :
PAX3 Antibody (N-Terminus) LS-C9504
catalog :
LS-C9504
clonality :
polyclonal
host :
domestic rabbit
conjugate :
nonconjugated
reactivity :
human, mouse
application :
western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation, immunohistochemistry - paraffin section, immunohistochemistry - frozen section
product information
AntibodyID :
10245
AntibodyName :
LS-C9504
TargetSpecies :
Mouse
Host Species :
Rabbit
Product Name :
PAX3 Antibody (N-Terminus) LS-C9504
Specificity :
Recognizes the ~53kD Pax3 protein in Pax3-MSCV transduced NIH-3T3 cell lysates on a Western blot assay. An untransduced NIH-3T3 cell line did not exhibit the target ~53kD band. However, a strong non-specific band at ~70kD and several other faint bands were observed in both the positive and negative control cell lines. Has also been used in immunofluorescence assays using Pax3-FKHR infected NIH-3T3 cells that were fixed in 2% paraformaldehyde and permeabilized in 2% paraformaldehyde containing 1% Triton X-100. Species sequence homology: human and chicken.
ClonalityDesc :
Polyclonal
AntibodyModification :
Unconjugated
AntigenModification :
N-Terminus
PresentationDesc :
Supplied a liquid, 0.1% sodium azide.
ImmunogenType :
Recombinant protein
ImmunogenDesc :
His6-tagged recombinant protein derived from a portion of the N-Terminus of mouse Pax3 sequence, encompassing the paired box and homeobox domains.
PurificationDesc :
Antiserum
RecommendedStorageDesc :
Short term: store at 4°C. Long term: aliquot and store at -20°C. Avoid freeze-thaw cycles.
IsotypeName :
IgG
Gene :
PAX3
StandardGeneSymbol :
PAX3
Reactivity :
Mouse
Usage :
IF (1:50 - 1:250), IHC, IHC-Fr, IHC-P (1:100 - 1:500), IP (1:50 - 1:250), WB (1:50 - 1:250)
ShortWebDescription :
PAX3 antibody LS-C9504 is an unconjugated rabbit polyclonal antibody to mouse PAX3 (N-Terminus). Validated for IF, IHC, IP and WB.
UsageText :
Suitable for use in Immunofluorescence, Western Blot, Immunoprecipitation and Immunohistochemistry. Immunofluorescence: 1:50-1:250. Western Blot: 1:50-1:250. Immunoprecipitation: 1:50-1:250. Immunohistochemistry (Frozen and paraffin): 1:50-1:250. Use with formalin-fixed, paraffin-embedded mouse tissue sections, requires heat induced epitope retrieval (HIER) using citrate buffer, pH 6.0 for 10 minutes.
Synonyms :
PAX3, CDHS, Paired domain gene 3, Paired box protein Pax-3, Paired domain gene HuP2, Waardenburg syndrome 1, WS1, HUP2, Paired box 3, Paired box homeotic gene 3, WS3
SalesRegion :
Worldwide
company information
LifeSpan Biosciences
2401 Fourth Avenue, Suite 900
Seattle, WA 98121
CustomerSupport@lsbio.com
https://www.lsbio.com
1-206-464-1554
headquarters: USA
Since 1995, LifeSpan has been the industry leader in molecular pathology, specializing in the localization of proteins in normal and diseased tissues, both human and non-human. We offer more than 74,000 antibodies, custom designed immunohistochemistry (IHC) studies, immediately available human tissue IHC profiles for more than 500 proteins, and histology and pathology services. Our bank of 2 million specimens is available to support our customers' contract research studies and contains frozen and formalin-fixed (FFPE) normal and diseased tissues. Our contract services are comprehensive; they include study design, antibody sourcing and characterization, tissue sourcing and validation, immunolabeling, trouble shooting, and interpretation of the results by a LifeSpan pathologist.