This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
LifeSpan Biosciences
product type :
antibody
product name :
CD274 / B7-H1 / PD-L1 Antibody (clone MIH6, FITC) LS-C58198
catalog :
LS-C58198
clonality :
monoclonal
host :
rat
conjugate :
FITC
clone name :
MIH6
reactivity :
human, mouse
application :
immunohistochemistry, immunoprecipitation, flow cytometry, immunohistochemistry - frozen section
product information
AntibodyName :
LS-C58198
TargetSpecies :
Mouse
Host Species :
Rat
Product Name :
CD274 / B7-H1 / PD-L1 Antibody (clone MIH6, FITC) LS-C58198
Specificity :
Detects mouse CD274, also known as B7-H1 and PD-1L, a cell surface glycoprotein which is a member of the B7 family of co-stimulatory molecules. CD274 is expressed constitutively on macrophages and dendritic cells, and is induced on activated T-cells, B-cells, endothelial cells and epithelial cells in response to Interferons alpha, beta and gamma. CD274 is reported to possess dual functions; inhibition of activated effector T cells and co-stimulation of naive T cells. CD274 inhibits proliferation of activated T cells via ligation to the co-inhibitory molecule CD279 (programmed death-1; PD-1) leading to the secretion of the regulatory cytokine interleukin-10. CD274 has also been shown to costimulate early T cell priming and differentiation. Deregulated CD274 function has been reported in chronic viral and intracellular bacterial infection, as well as in many autoimmune diseases and cancers.
ClonalityDesc :
Monoclonal
CloneName :
MIH6
AntibodyModification :
FITC
PresentationDesc :
PBS, pH 7.4, 0.09% Sodium Azide, 1% BSA
ImmunogenDesc :
Mouse CD274 - transfected L5178Y cells.
PurificationDesc :
Affinity purified
RecommendedStorageDesc :
Short term: store at 4°C. Long term: aliquot and store -20°C. Avoid freeze-thaw cycles. Protect from light. Store undiluted.
IsotypeName :
IgG2a
Gene :
CD274 / B7-H1 / PD-L1
StandardGeneSymbol :
CD274
Reactivity :
Mouse
Usage :
Flo (1:1), IHC, IHC-Fr, IP
ShortWebDescription :
PD-L1 antibody LS-C58198 is an FITC-conjugated rat monoclonal antibody to mouse PD-L1 (CD274 / B7-H1). Validated for Flow, IHC and IP.
UsageText :
The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested. Flow Cytometry: Use 10 ul of the suggested working dilution to label 1x10^6 cells in 100 ul. The Fc region of monoclonal antibodies may bind non-specifically to cells expressing low affinity Fc receptors.
Synonyms :
CD274, B7-H, B7 homolog 1, B7-H1, B7H1, CD274 molecule, CD274 antigen, PDCD1 ligand 1, PDL1, PD-L1, PDCD1L1, PDCD1LG1, Programmed death ligand 1
company information
LifeSpan Biosciences
2401 Fourth Avenue, Suite 900
Seattle, WA 98121
antibodies@lsbio.com
https://www.lsbio.com
1-206-464-1554
headquarters: USA
Since 1995, LifeSpan has been the industry leader in molecular pathology, specializing in the localization of proteins in normal and diseased tissues, both human and non-human. We offer more than 74,000 antibodies, custom designed immunohistochemistry (IHC) studies, immediately available human tissue IHC profiles for more than 500 proteins, and histology and pathology services. Our bank of 2 million specimens is available to support our customers' contract research studies and contains frozen and formalin-fixed (FFPE) normal and diseased tissues. Our contract services are comprehensive; they include study design, antibody sourcing and characterization, tissue sourcing and validation, immunolabeling, trouble shooting, and interpretation of the results by a LifeSpan pathologist.