product summary
request information :
company name :
LifeSpan Biosciences
product type :
antibody
product name :
Anti-SHH
catalog :
LS-C10143
quantity :
price :
clonality :
polyclonal
host :
rabbit
reactivity :
human, mouse
application :
western blot, ELISA, immunohistochemistry, neutralization
more info or order :
LifeSpan Biosciences product webpage
product information
?AntibodyID :
10884
AntibodyName :
LS-C10143
TargetSpecies :
mouse
HostSpecies :
rabbit
ProductName :
Anti-SHH
Specificity :
Recognizes mouse rmShh N-terminal peptide. In western blots, ~50% cross-reactivity with the N-terminal peptide from recombinant mouse Dhh (aa 23-198), no cross-reactivity with rmIhh (aa 66-240), and no cross-reactivity with the C-terminal peptides of mDhh (aa 199-396) or mShh (aa 199-437). Species cross-reactivity: human.
ClonalityDesc :
polyclonal
AntigenModification :
aa25-198
PresentationDesc :
PBS, pH 7.2, 5% trehalose, 40% glycerol.
ImmunogenType :
Recombinant protein
ImmunogenDesc :
E coli derived rmShh N-terminal peptide (aa25-198).
PurificationDesc :
Protein G Column
RecommendedStorageDesc :
Long term: -20°C; Short term: +4°C. Avoid repeat freeze-thaw cycles.
IsotypeName :
IgG2a
gene :
SHH
StandardGeneSymbol :
SHH
gene family :
protease
Subfamily :
Cysteine C46
reactivity :
Human, Mouse
Usage :
ELISA (4 ug/ml), IHC-Fr, Neut, WB (1 - 2 ug/ml)
UsageText :
Suitable for use in ELISA, Western Blot, Immunohistochemistry, Neutralization. ELISA: 4 ug/ml, using 100 ul/well and 100 ul/well of biotinylated detection antibody. Immunohistochemistry: 25 ug/ml with appropriate secondary reagents to detect Shh in frozen mouse brain tissue sections. Western Blot: 1-2 ug/ml. Using a colorimetric detection system, the detection limit for rmShh N-terminal peptide is approximately 50 ng/lane under non-reducing and reducing conditions. Chemiluminescent detection with WesternGlo Chemiluminescent Detection Substrate will increase sensitivity by 5 to 50 fold.
Synonyms :
SHH, HHG1, HLP3, HPE3, MCOPCB5, SMMCI, Sonic Hedgehog, TPT, TPTPS
SwissProtProtoID :
Q15465
more info or order :
LifeSpan Biosciences product webpage
company information
LifeSpan Biosciences
2401 Fourth Avenue, Suite 900
Seattle, WA 98121
antibodies@lsbio.com
www.lsbio.com
1-206-464-1554
headquarters: USA
Since 1995, LifeSpan has been the industry leader in molecular pathology, specializing in the localization of proteins in normal and diseased tissues, both human and non-human. We offer more than 74,000 antibodies, custom designed immunohistochemistry (IHC) studies, immediately available human tissue IHC profiles for more than 500 proteins, and histology and pathology services. Our bank of 2 million specimens is available to support our customers' contract research studies and contains frozen and formalin-fixed (FFPE) normal and diseased tissues. Our contract services are comprehensive; they include study design, antibody sourcing and characterization, tissue sourcing and validation, immunolabeling, trouble shooting, and interpretation of the results by a LifeSpan pathologist.