product summary
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company name :
LifeSpan Biosciences
product type :
antibody
product name :
PathPlus™ VPS35 Antibody (aa783-796) LS-B5909
catalog :
LS-B5909
clonality :
polyclonal
host :
domestic goat
conjugate :
nonconjugated
reactivity :
human
application :
western blot, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section
citations: 1
Reference
Baeken M, Weckmann K, Diefenthäler P, Schulte J, Yusifli K, Moosmann B, et al. Novel Insights into the Cellular Localization and Regulation of the Autophagosomal Proteins LC3A, LC3B and LC3C. Cells. 2020;9: pubmed publisher
image
image 1 :
LifeSpan Biosciences LS-B5909 image 1
Anti-VPS35 antibody IHC of human breast, epithelium. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 3.75 ug/ml.
image 2 :
LifeSpan Biosciences LS-B5909 image 2
VPS35 / MEM3 antibody (1µg/ml) staining of Human Brain (Cerebellum) (A) and Rat Brain (B) lysates (35µg protein in RIPA buffer). Detected by chemiluminescence.
image 3 :
LifeSpan Biosciences LS-B5909 image 3
VPS35 / MEM3 antibody immunofluorescence analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).
product information
AntibodyID :
141316
AntibodyName :
LS-B5909
TargetSpecies :
Human
Host Species :
Goat
Product Name :
PathPlus™ VPS35 Antibody (aa783-796) LS-B5909
Specificity :
Human VPS35. Note there is a hypothetical protein called similar to vacuolar protein sorting 35 (XP_040192.1), which is virtually identical.
ClonalityDesc :
Polyclonal
AntibodyModification :
Unconjugated
AntigenModification :
aa783-796
PresentationDesc :
TBS, pH 7.3, 0.02% Sodium Azide, 0.5% BSA
ImmunogenDesc :
Peptide with sequence C-SPESEGPIYEGLIL, from the C-Terminus of protein sequence according to NP_060676.2.
PurificationDesc :
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide
RecommendedStorageDesc :
Aliquot and store at -20°C. Avoid freeze-thaw cycles.
Gene :
VPS35
BlockingPeptide :
LS-E25680
StandardGeneSymbol :
VPS35
Reactivity :
Rabbit, Mouse, Bovine, Rat, Hamster, Horse, Human, Monkey
Usage :
IF (10 µg/ml), IHC, IHC-P (3.75 µg/ml), Peptide-ELISA (1:128000), WB (1 - 3 µg/ml)
ShortWebDescription :
VPS35 antibody LS-B5909 is an unconjugated goat polyclonal antibody to VPS35 (aa783-796) from human. It is reactive with human, mouse, rat and other species. Validated for IF, IHC, Peptide-ELISA and WB. Cited in 2 publications.
Synonyms :
VPS35, MEM3, Maternal-embryonic 3, Vesicle protein sorting 35, PARK17, HVPS35
SalesRegion :
Worldwide
company information
LifeSpan Biosciences
2401 Fourth Avenue, Suite 900
Seattle, WA 98121
CustomerSupport@lsbio.com
https://www.lsbio.com
1-206-464-1554
headquarters: USA
Since 1995, LifeSpan has been the industry leader in molecular pathology, specializing in the localization of proteins in normal and diseased tissues, both human and non-human. We offer more than 74,000 antibodies, custom designed immunohistochemistry (IHC) studies, immediately available human tissue IHC profiles for more than 500 proteins, and histology and pathology services. Our bank of 2 million specimens is available to support our customers' contract research studies and contains frozen and formalin-fixed (FFPE) normal and diseased tissues. Our contract services are comprehensive; they include study design, antibody sourcing and characterization, tissue sourcing and validation, immunolabeling, trouble shooting, and interpretation of the results by a LifeSpan pathologist.