product summary
request information :
company name :
LifeSpan Biosciences
product type :
antibody
product name :
Anti-MSH6
catalog :
LS-B5558
quantity :
price :
clonality :
monoclonal
host :
mouse
reactivity :
human
application :
immunohistochemistry - paraffin section, immunohistochemistry, immunohistochemistry - frozen section
more info or order :
LifeSpan Biosciences product webpage
image
image 1 :
LifeSpan Biosciences LS-B5558 image 1
Human Intestine: Formalin-Fixed, Paraffin-Embedded (FFPE)
image 2 :
LifeSpan Biosciences LS-B5558 image 2
Human Tonsil: Formalin-Fixed, Paraffin-Embedded (FFPE)
product information
?AntibodyID :
140164
AntibodyName :
LS-B5558
TargetSpecies :
Human
Host Species :
Mouse
Product Name :
Anti-MSH6
Specificity :
MSH6 is a mismatch repair gene which is deficient in a high proportion of patients with microsatellite instability (MSI-H). This finding is associated with the autosomal dominant condition known as Hereditary Non-Polyposis Colon Cancer (HNPCC). The anti-MSH6 antibody is useful in screening patients and families for this condition. Colon cancers that are microsatellite unstable have a better prognosis than their microsatellite stable counterparts.
ClonalityDesc :
Monoclonal
CloneName :
44
PresentationDesc :
Tissue culture supernatant in PBS, pH 7.4, protein base, preserved, sodium azide
PurificationDesc :
Tissue culture supernatant
RecommendedStorageDesc :
Short term 4°C, long term aliquot and store at -20°C, avoid freeze thaw cycles.
IsotypeName :
IgG1
Gene :
MSH6
StandardGeneSymbol :
MSH6
Reactivity :
Human
Usage :
IHC-Fr, IHC-P (1:50)
UsageText :
Immunohistochemistry: Paraffin, Frozen Recommended dilution: 1:25-1:100. Preparation and Pretreatment 1. Cut 3-4 ^m section of formalin-fixed paraffin-embedded tissue and place on positively charged slides; dry overnight at 58°C. 2. Deparaffinize, rehydrate, and epitope retrieve; the preferred method is the use of Heat Induced Epitope Retrieval (HIER) techniques in conjunction with a pressure cooker. The preferred method allows for simultaneous deparaffinization, rehydration, and epitope retrieval. Upon completion, rinse with 5 changes of distilled or deionized water. 3. If using HRP detection system, place slides in peroxide block for 10 minutes; rinse. If using AP detection system, omit this step. Positive control Colon Carcinoma, Colon Mucosa Staining pattern Nuclear.
Synonyms :
MSH6, GTBP, HSAP, GTMBP, MutS (E. coli) homolog 6, HMSH6, Sperm-associated protein, p160, G/T mismatch-binding protein, HNPCC5, MutS homolog 6 (E. coli), MutS-alpha 160 kDa subunit
SwissProtProtoID :
P52701
more info or order :
LifeSpan Biosciences product webpage
company information
LifeSpan Biosciences
2401 Fourth Avenue, Suite 900
Seattle, WA 98121
antibodies@lsbio.com
www.lsbio.com
1-206-464-1554
headquarters: usa
Since 1995, LifeSpan has been the industry leader in molecular pathology, specializing in the localization of proteins in normal and diseased tissues, both human and non-human. We offer more than 74,000 antibodies, custom designed immunohistochemistry (IHC) studies, immediately available human tissue IHC profiles for more than 500 proteins, and histology and pathology services. Our bank of 2 million specimens is available to support our customers' contract research studies and contains frozen and formalin-fixed (FFPE) normal and diseased tissues. Our contract services are comprehensive; they include study design, antibody sourcing and characterization, tissue sourcing and validation, immunolabeling, trouble shooting, and interpretation of the results by a LifeSpan pathologist.