product summary
request information :
company name :
LifeSpan Biosciences
product type :
antibody
product name :
Anti-HGF
catalog :
LS-B4012
quantity :
price :
clonality :
polyclonal
host :
rabbit
reactivity :
human
application :
immunohistochemistry - paraffin section, western blot, ELISA, immunohistochemistry
more info or order :
LifeSpan Biosciences product webpage
image
image 1 :
LifeSpan Biosciences LS-B4012 image 1
Adrenal, Human: Formalin-Fixed, Paraffin-Embedded (FFPE)
image 2 :
LifeSpan Biosciences LS-B4012 image 2
Liver, Human: Formalin-Fixed, Paraffin-Embedded (FFPE)
image 3 :
LifeSpan Biosciences LS-B4012 image 3
Placenta, Human: Formalin-Fixed, Paraffin-Embedded (FFPE)
product information
?AntibodyID :
115179
AntibodyName :
LS-B4012
TargetSpecies :
Human
Host Species :
Rabbit
Product Name :
Anti-HGF
Specificity :
Human HGF
ClonalityDesc :
Polyclonal
AntigenModification :
N-Terminus
PresentationDesc :
Lyophilized from PBS with 2% sucrose
ImmunogenType :
Synthetic peptide
ImmunogenDesc :
Synthetic peptide from N-terminus of human HGF (NP_001010932.1, P14210). Percent identity by BLAST analysis: Human, Mouse, Rat, Sheep, Dog, Bovine, Rabbit (100%); Guinea pig (92%).
PurificationDesc :
Immunoaffinity purified
RecommendedStorageDesc :
Long term: -20°C; Short term: +4°C. Avoid repeat freeze-thaw cycles.
ResuspensionLiquidDesc :
Distilled water
IsotypeName :
IgG
Gene :
HGF
StandardGeneSymbol :
HGF
gene family :
Protease
Subfamily :
Serine S1
Reactivity :
Bovine, Dog, Human, Mouse, Rabbit, Rat, Sheep
Usage :
IHC-P (5 µg/ml), WB (0.2 - 1 µg/ml)
UsageText :
Immunohistochemistry: LS-B4012 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B4012 was determined to be 5 ug/ml. Western Blot: Suggested dilution at 1µg/ml in 5% skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as second antibody. ELISA titer in peptide based assay: 1:312500.
Synonyms :
HGF, DFNB39, Hepatocyte growth factor, Hepatopoeitin-A, Hepapoietin A, Hepatopoietin-A, Scatter factor), SF, F-TCF, HGFB, HPTA, Scatter factor
SwissProtProtoID :
P14210
more info or order :
LifeSpan Biosciences product webpage
company information
LifeSpan Biosciences
2401 Fourth Avenue, Suite 900
Seattle, WA 98121
antibodies@lsbio.com
www.lsbio.com
1-206-464-1554
headquarters: usa
Since 1995, LifeSpan has been the industry leader in molecular pathology, specializing in the localization of proteins in normal and diseased tissues, both human and non-human. We offer more than 74,000 antibodies, custom designed immunohistochemistry (IHC) studies, immediately available human tissue IHC profiles for more than 500 proteins, and histology and pathology services. Our bank of 2 million specimens is available to support our customers' contract research studies and contains frozen and formalin-fixed (FFPE) normal and diseased tissues. Our contract services are comprehensive; they include study design, antibody sourcing and characterization, tissue sourcing and validation, immunolabeling, trouble shooting, and interpretation of the results by a LifeSpan pathologist.