product summary
request information :
company name :
LifeSpan Biosciences
product type :
antibody
product name :
Anti-PRKDC / DNA-PKcs
catalog :
LS-B3750
quantity :
price :
clonality :
polyclonal
host :
rabbit
antigen modification :
acetylated
reactivity :
human
application :
immunohistochemistry - paraffin section, western blot, immunohistochemistry
more info or order :
LifeSpan Biosciences product webpage
image
image 1 :
LifeSpan Biosciences LS-B3750 image 1
Prostate, Human: Formalin-Fixed, Paraffin-Embedded (FFPE)
image 2 :
LifeSpan Biosciences LS-B3750 image 2
Testis, Human: Formalin-Fixed, Paraffin-Embedded (FFPE)
product information
?AntibodyID :
111769
AntibodyName :
LS-B3750
TargetSpecies :
Human
Host Species :
Rabbit
Product Name :
Anti-PRKDC / DNA-PKcs
Specificity :
Detects a >350 kD protein, corresponding to the apparent molecular mass of DNA-PKcs on SDS-PAGE immunoblots. Also detects an ~180 kD band, believed to be a processed form of the catalytic subunit.
ClonalityDesc :
Polyclonal
AntigenModification :
aa4085-4096
PresentationDesc :
PBS, pH 7.2, 50% glycerol, 0.09% azide
ImmunogenType :
Synthetic peptide - KLH conjugated
ImmunogenDesc :
An 11 residue synthetic peptide (C)GRTWEGWEPWM based on the human DNA-PKcs (residues 4085-4096) with the cysteine (C) residue added and the peptide coupled to KLH. Percent identity by BLAST analysis: Human, Gorilla, Monkey, Marmoset, Mouse, Hamster, Bovine, Bat, Pig (100%); Dog, Horse, Turkey, Chicken, Trichoplax (91%); Panda, Pufferfish, Capsaspora (82%).
PurificationDesc :
Immunoaffinity purified
RecommendedStorageDesc :
Short term 4°C, long term aliquot and store at -20°C, avoid freeze thaw cycles.
Gene :
PRKDC / DNA-PKcs
StandardGeneSymbol :
PRKDC
gene family :
Protein Kinase
Subfamily :
PI3/PI4
Reactivity :
Bat, Bovine, Gorilla, Hamster, Human, Monkey, Mouse, Pig
Usage :
IHC-P (10 µg/ml), WB (2 µg/ml)
UsageText :
Immunohistochemistry: LS-B3750 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B3750 was determined to be 10 ug/ml.
Synonyms :
PRKDC, DNA-PK, DNAPK, DNPK1, HYRC, HYRC1, p350, Scid, XRCC7, DNA-PK catalytic subunit, DNA-PKcs, p460
more info or order :
LifeSpan Biosciences product webpage
company information
LifeSpan Biosciences
2401 Fourth Avenue, Suite 900
Seattle, WA 98121
antibodies@lsbio.com
www.lsbio.com
1-206-464-1554
headquarters: USA
Since 1995, LifeSpan has been the industry leader in molecular pathology, specializing in the localization of proteins in normal and diseased tissues, both human and non-human. We offer more than 74,000 antibodies, custom designed immunohistochemistry (IHC) studies, immediately available human tissue IHC profiles for more than 500 proteins, and histology and pathology services. Our bank of 2 million specimens is available to support our customers' contract research studies and contains frozen and formalin-fixed (FFPE) normal and diseased tissues. Our contract services are comprehensive; they include study design, antibody sourcing and characterization, tissue sourcing and validation, immunolabeling, trouble shooting, and interpretation of the results by a LifeSpan pathologist.