This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
LifeSpan Biosciences
product type :
antibody
product name :
NCSTN / Nicastrin Antibody (clone AT3B2) IHC-plus™ LS-B3583
catalog :
LS-B3583
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
AT3B2
reactivity :
human
application :
western blot, ELISA, immunohistochemistry, immunohistochemistry - paraffin section
product information
?AntibodyID :
109469
AntibodyName :
LS-B3583
TargetSpecies :
Human
Host Species :
Mouse
Product Name :
NCSTN / Nicastrin Antibody (clone AT3B2) IHC-plus™ LS-B3583
Specificity :
human Nicastrin (34-153aa)
ClonalityDesc :
Monoclonal
CloneName :
AT3B2
AntibodyModification :
Unconjugated
PresentationDesc :
PBS, pH 7.4, 0.1% sodium azide
ImmunogenType :
Recombinant protein
ImmunogenDesc :
Recombinant human Nicastrin (34-153aa) purified from E. coli
PurificationDesc :
Protein G purified
RecommendedStorageDesc :
Aliquot and store at -20°C. Avoid freeze-thaw cycles.
IsotypeName :
IgG2b,k
Gene :
NCSTN / Nicastrin
StandardGeneSymbol :
NCSTN
gene family :
Protease
Reactivity :
Mouse, Human
Usage :
ELISA, IHC, IHC-P (5 µg/ml), WB (1:500 - 1:1000)
UsageText :
Immunohistochemistry: LS-B3583 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B3583 was determined to be 5 ug/ml. Western Blot: Extracts of Mouse brain (80ug) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human Nicastrin (1:500). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
Synonyms :
NCSTN, Anterior pharynx-defective 2, ATAG1874, KIAA0253, Nicastrin, APH2, RP11-517F10.1
company information
LifeSpan Biosciences
2401 Fourth Avenue, Suite 900
Seattle, WA 98121
antibodies@lsbio.com
https://www.lsbio.com
1-206-464-1554
headquarters: USA
Since 1995, LifeSpan has been the industry leader in molecular pathology, specializing in the localization of proteins in normal and diseased tissues, both human and non-human. We offer more than 74,000 antibodies, custom designed immunohistochemistry (IHC) studies, immediately available human tissue IHC profiles for more than 500 proteins, and histology and pathology services. Our bank of 2 million specimens is available to support our customers' contract research studies and contains frozen and formalin-fixed (FFPE) normal and diseased tissues. Our contract services are comprehensive; they include study design, antibody sourcing and characterization, tissue sourcing and validation, immunolabeling, trouble shooting, and interpretation of the results by a LifeSpan pathologist.