product summary
request information :
company name :
LifeSpan Biosciences
product type :
antibody
product name :
Anti-B7-H1 / PD-L1 / CD274
catalog :
LS-B2999
quantity :
price :
clonality :
polyclonal
host :
goat
reactivity :
human
application :
immunohistochemistry - paraffin section, western blot, ELISA, immunohistochemistry
more info or order :
LifeSpan Biosciences product webpage
image
image 1 :
LifeSpan Biosciences LS-B2999 image 1
Human Heart (formalin-fixed, paraffin-embedded) stained with CD274 antibody LS-B2999 at 4 ug/ml followed by biotinylated anti-goat IgG secondary antibody LS-D3, alkaline phosphatase-streptavidin and chromogen.
image 2 :
LifeSpan Biosciences LS-B2999 image 2
Human Skeletal Muscle (formalin-fixed, paraffin-embedded) stained with CD274 antibody LS-B2999 at 4 ug/ml followed by biotinylated anti-goat IgG secondary antibody LS-D3, alkaline phosphatase-streptavidin and chromogen.
product information
?AntibodyID :
97376
AntibodyName :
LS-B2999
TargetSpecies :
Human
Host Species :
Goat
Product Name :
Anti-B7-H1 / PD-L1 / CD274
Specificity :
Human CD274.
ClonalityDesc :
Polyclonal
AntigenModification :
C-Terminus
PresentationDesc :
Tris-buffered saline, pH 7.3, 0.5% BSA, 0.02% sodium azide
ImmunogenType :
Synthetic peptide
ImmunogenDesc :
Synthetic peptide C-KKQSDTHLEET from the C-terminus of human CD274 (NP_054862.1). Percent identity by BLAST analysis: Human, Gorilla (100%); Gibbon (91%); Monkey, Marmoset (82%).
PurificationDesc :
Immunoaffinity Chromatography
RecommendedStorageDesc :
Store at -20°C. Minimize freezing and thawing.
Gene :
B7-H1 / PD-L1 / CD274
StandardGeneSymbol :
CD274
Reactivity :
Human
Usage :
ELISA (1:32000), IHC-P (4 µg/ml), WB (0.3 - 2 µg/ml)
UsageText :
Immunohistochemistry: LS-B2999 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2999 was determined to be 4 ug/ml.
Synonyms :
CD274, B7-H, B7 homolog 1, B7-H1, B7H1, CD274 molecule, CD274 antigen, PDCD1 ligand 1, PDL1, PD-L1, PDCD1L1, PDCD1LG1, Programmed death ligand 1
SwissProtProtoID :
Q9NZQ7
more info or order :
LifeSpan Biosciences product webpage
company information
LifeSpan Biosciences
2401 Fourth Avenue, Suite 900
Seattle, WA 98121
antibodies@lsbio.com
www.lsbio.com
1-206-464-1554
headquarters: usa
Since 1995, LifeSpan has been the industry leader in molecular pathology, specializing in the localization of proteins in normal and diseased tissues, both human and non-human. We offer more than 74,000 antibodies, custom designed immunohistochemistry (IHC) studies, immediately available human tissue IHC profiles for more than 500 proteins, and histology and pathology services. Our bank of 2 million specimens is available to support our customers' contract research studies and contains frozen and formalin-fixed (FFPE) normal and diseased tissues. Our contract services are comprehensive; they include study design, antibody sourcing and characterization, tissue sourcing and validation, immunolabeling, trouble shooting, and interpretation of the results by a LifeSpan pathologist.