IHC-plus™ VCL / Vinculin Antibody LS-B4848
LifeSpan Biosciences
catalog: LS-B4848

domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunohistochemistry - paraffin section

Sample (30 ug of whole cell lysate). A: A431. B: H1299. 7.5% SDS PAGE. VCL antibody diluted at 1:1000

Anti-Vinculin antibody IHC of human heart. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.
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VCL / Vinculin Antibody LS-C124793
LifeSpan Biosciences
catalog: LS-C124793

mouse monoclonal
reactivity: human
application: western blot, immunohistochemistry, immunohistochemistry - frozen section

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VCL / Vinculin Antibody (phospho-Tyr100) LS-C40217
LifeSpan Biosciences
catalog: LS-C40217

domestic rabbit polyclonal
reactivity: human
modification: phosphorylated
application: western blot, immunoprecipitation

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VCL / Vinculin Antibody (phospho-Tyr822) LS-C40218
LifeSpan Biosciences
catalog: LS-C40218

domestic rabbit polyclonal
reactivity: human
modification: phosphorylated
application: western blot

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VCL / Vinculin Antibody (phospho-Tyr1065) LS-C40219
LifeSpan Biosciences
catalog: LS-C40219

domestic rabbit polyclonal
reactivity: human
modification: phosphorylated
application: western blot, immunoprecipitation

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VCL / Vinculin Antibody LS-C40210
LifeSpan Biosciences
catalog: LS-C40210

mouse monoclonal
reactivity: human
application: western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation, immunohistochemistry - frozen section

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VCL / Vinculin Antibody (clone V284) LS-C85522
LifeSpan Biosciences
catalog: LS-C85522

mouse monoclonal (V284)
reactivity: human
application: western blot

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VCL / Vinculin Antibody (clone VLN01, Azide-free) LS-C87611
LifeSpan Biosciences
catalog: LS-C87611

mouse monoclonal (VLN01)
reactivity: human
application: western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation, immunohistochemistry - paraffin section

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VCL / Vinculin Antibody (aa12-39) LS-C100624
LifeSpan Biosciences
catalog: LS-C100624

domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunohistochemistry - paraffin section

Western blot of anti-VINC Antibody in mouse lung tissue lysates (35 ug/lane). VINC (arrow) was detected using the purified antibody.

Formalin-fixed and paraffin-embedded human skeletal muscle tissue reacted with VINC antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
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VCL / Vinculin Antibody (aa1012-1039) LS-C100625
LifeSpan Biosciences
catalog: LS-C100625

domestic rabbit polyclonal
reactivity: human
application: western blot

Western blot of anti-VINC Antibody in Ramos cell line lysates (35 ug/lane). VINC (arrow) was detected using the purified antibody.
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IHC-plus™ VCL / Vinculin Antibody (Azide-free) LS-B4764
LifeSpan Biosciences
catalog: LS-B4764

mouse monoclonal
reactivity: human
application: western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation, immunohistochemistry - paraffin section

Anti-Vinculin antibody IHC of human prostate. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 2.5 ug/ml. This image was taken for the unmodified form of this product. Other forms have not been tested.

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VCL / Vinculin Antibody (clone FB11) LS-C124794
LifeSpan Biosciences
catalog: LS-C124794

mouse monoclonal (FB11)
reactivity: human
application: western blot, ELISA, immunohistochemistry, immunohistochemistry - frozen section

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VCL / Vinculin Antibody (clone VIN-54) LS-C124795
LifeSpan Biosciences
catalog: LS-C124795

mouse monoclonal (VIN-54)
reactivity: human
application: western blot

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VCL / Vinculin Antibody (aa873-922) LS-C135920
LifeSpan Biosciences
catalog: LS-C135920

domestic rabbit polyclonal
reactivity: human
application: western blot

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IHC-plus™ VCL / Vinculin Antibody (aa786-835) LS-B5770
LifeSpan Biosciences
catalog: LS-B5770

domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section

Anti-Vinculin antibody IHC of human heart. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:100.

Immunofluorescence analysis of A549 cells, using Vinculin Antibody. The picture on the right is blocked with the synthesized peptide.

Western blot analysis of lysates from HeLa cells, treated with forskolin 40nM 30', using Vinculin Antibody. The lane on the right is blocked with the synthesized peptide.
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IHC-plus™ VCL / Vinculin Antibody (clone VLN01) LS-B8515
LifeSpan Biosciences
catalog: LS-B8515

mouse monoclonal (VLN01)
reactivity: human
application: western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation, immunohistochemistry - paraffin section

Anti-Vinculin antibody IHC of human heart, intercalated disk. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 5 ug/ml.
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VCL / Vinculin Antibody (clone VIN-54) LS-C171297
LifeSpan Biosciences
catalog: LS-C171297

mouse monoclonal (VIN-54)
reactivity: human
application: western blot, immunohistochemistry, immunohistochemistry - frozen section

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VCL / Vinculin Antibody (clone VIN-54, Magnetic beads) LS-C171298
LifeSpan Biosciences
catalog: LS-C171298

mouse monoclonal (VIN-54)
reactivity: human
conjugate: Bead
application: immunoprecipitation

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VCL / Vinculin Antibody (clone VIN-54, Sepharose) LS-C171299
LifeSpan Biosciences
catalog: LS-C171299

mouse monoclonal (VIN-54)
reactivity: human
application: immunoprecipitation

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VCL / Vinculin Antibody (aa1-1067, clone 10B2533, Azide-free) LS-C183723
LifeSpan Biosciences
catalog: LS-C183723

mouse monoclonal (10B2533)
reactivity: human
application: western blot, ELISA, immunocytochemistry

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VCL / Vinculin Antibody (clone 489220) LS-C195330
LifeSpan Biosciences
catalog: LS-C195330

mouse monoclonal (489220)
reactivity: human
application: western blot, immunohistochemistry, immunocytochemistry

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VCL / Vinculin Antibody (clone 2C6-1B5) LS-C198423
LifeSpan Biosciences
catalog: LS-C198423

mouse monoclonal (2C6-1B5)
reactivity: human
application: western blot, ELISA, immunocytochemistry

VCL monoclonal antibody (M01), clone 2C6-1B5 Western Blot analysis of VCL expression in HeLa.

Immunofluorescence of monoclonal antibody to VCL on HeLa cell. [antibody concentration 10 ug/ml]
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VCL / Vinculin Antibody (phospho-Tyr821) LS-C199725
LifeSpan Biosciences
catalog: LS-C199725

domestic rabbit polyclonal
reactivity: human
modification: phosphorylated
application: immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section

Immunofluorescence analysis of HepG2 cells, using Vinculin (Phospho-Tyr821) Antibody. The picture on the right is blocked with the phospho peptide.

Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using Vinculin (Phospho-Tyr821) Antibody. The picture on the right is blocked with the phospho peptide.
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VCL / Vinculin Antibody (clone FB11, Concentrated) LS-C202542
LifeSpan Biosciences
catalog: LS-C202542

mouse monoclonal (FB11)
reactivity: human
application: western blot, immunohistochemistry, immunohistochemistry - frozen section

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IHC-plus™ VCL / Vinculin Antibody (aa356-607) LS-B9609
LifeSpan Biosciences
catalog: LS-B9609

domestic rabbit polyclonal
reactivity: human
application: western blot, ELISA, immunohistochemistry, immunohistochemistry - paraffin section

Human Prostate, Smooth Muscle: Formalin-Fixed, Paraffin-Embedded (FFPE)
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VCL / Vinculin Antibody (HRP) LS-C211449
LifeSpan Biosciences
catalog: LS-C211449

domestic rabbit polyclonal
reactivity: human
conjugate: HRP
application: western blot, ELISA

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VCL / Vinculin Antibody (FITC) LS-C211450
LifeSpan Biosciences
catalog: LS-C211450

domestic rabbit polyclonal
reactivity: human
conjugate: FITC
application: western blot, ELISA

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VCL / Vinculin Antibody (Biotin) LS-C211451
LifeSpan Biosciences
catalog: LS-C211451

domestic rabbit polyclonal
reactivity: human
conjugate: biotin
application: western blot, ELISA

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VCL / Vinculin Antibody (HRP) LS-C212066
LifeSpan Biosciences
catalog: LS-C212066

domestic rabbit polyclonal
reactivity: human
conjugate: HRP
application: western blot, ELISA, immunohistochemistry

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VCL / Vinculin Antibody (Biotin) LS-C212070
LifeSpan Biosciences
catalog: LS-C212070

domestic rabbit polyclonal
reactivity: human
conjugate: biotin
application: western blot, ELISA, immunohistochemistry

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IHC-plus™ VCL / Vinculin Antibody LS-B10133
LifeSpan Biosciences
catalog: LS-B10133

domestic rabbit polyclonal
reactivity: human
application: western blot, ELISA, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section

Western blot analysis of Vinculin in PC-3 cell lysate with Vinculin antibody at (A) 1 and (B) 2 ug/ml.

Immunofluorescence of Vinculin in rat small intestine tissue with Vinculin antibody at 20 ug/ml.

Anti-VCL / Vinculin antibody IHC staining of human heart. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.
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VCL / Vinculin Antibody (aa1016-1066) LS-C286911
LifeSpan Biosciences
catalog: LS-C286911

domestic rabbit polyclonal
reactivity: human
application: immunohistochemistry

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VCL / Vinculin Antibody (aa750-800) LS-C289272
LifeSpan Biosciences
catalog: LS-C289272

domestic rabbit polyclonal
reactivity: human
application: western blot

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VCL / Vinculin Antibody (aa1020-1134) LS-C296985
LifeSpan Biosciences
catalog: LS-C296985

domestic rabbit polyclonal
reactivity: human
application: western blot

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VCL / Vinculin Antibody (aa1020-1134, Biotin) LS-C299575
LifeSpan Biosciences
catalog: LS-C299575

domestic rabbit polyclonal
reactivity: human
conjugate: biotin
application: western blot

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VCL / Vinculin Antibody (aa173-188) LS-C312927
LifeSpan Biosciences
catalog: LS-C312927

domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunohistochemistry - paraffin section

WB of VCL / Vinculin antibody. All lanes: Anti-VCL at 0.5ug/ml. Lane 1: Rat Heart Tissue Lysate at 40ug. Lane 2: Rat Brain Tissue Lysate at 40ug. Lane 3: Rat Liver Tissue Lysate at 40ug. Lane 4: U87 Whole Cell Lysate at 40ug. Lane 5: SMMC Whole Cell Lysate at 40ug. Lane 6: HEPA Whole Cell Lysate at 40ug. Lane 7: HELA Whole Cell Lysate at 40ug. Lane 8: HT1080 Whole Cell Lysate at 40ug. Predicted bind size: 124KD. Observed bind size: 124KD.

VCL / Vinculin antibody. IHC(P): Rat Intestine Tissue.
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VCL / Vinculin Antibody (aa879-1064) LS-C314838
LifeSpan Biosciences
catalog: LS-C314838

domestic rabbit polyclonal
reactivity: human, mouse
application: western blot

Western Blot; Sample:Human Lung lysate; Primary Ab: 2µg/ml Rabbit Anti-Mouse VCL Antibody Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody

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VCL / Vinculin Antibody LS-C331674
LifeSpan Biosciences
catalog: LS-C331674

domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry

Western blot analysis of extracts of various cell lines.

Immunohistochemistry of paraffin-embedded human smooth muscle using VCL Antibodyat dilution of 1:100 (40x lens).
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VCL / Vinculin Antibody (C-Terminus) LS-C345584
LifeSpan Biosciences
catalog: LS-C345584

domestic rabbit polyclonal
reactivity: human
application: western blot

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IHC-plus™ VCL / Vinculin Antibody LS-B12145
LifeSpan Biosciences
catalog: LS-B12145

domestic rabbit polyclonal
reactivity: human
application: western blot, ELISA, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section

Human Testis: Formalin-Fixed, Paraffin-Embedded (FFPE)

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VCL / Vinculin Antibody (Internal) LS-C358386
LifeSpan Biosciences
catalog: LS-C358386

domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section

Western blot analysis of Vinculin expression in MCF7 (A); HepG2 (B) whole cell lysates.

Immunohistochemical analysis of Vinculin staining in human heart formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP-conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.

Immunofluorescent analysis of Vinculin staining in HepG2 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 deg C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
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IHC-plus™ VCL / Vinculin Antibody (aa1016-1066) LS-B12965
LifeSpan Biosciences
catalog: LS-B12965

domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunoprecipitation, immunohistochemistry - paraffin section

Human Kidney: Formalin-Fixed, Paraffin-Embedded (FFPE)
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VCL / Vinculin Antibody (760-840 aa) LS-C381738
LifeSpan Biosciences
catalog: LS-C381738

domestic rabbit polyclonal
reactivity: human
modification: phosphorylated
application: ELISA, immunohistochemistry, immunocytochemistry

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VCL / Vinculin Antibody (760-840 aa) LS-C386654
LifeSpan Biosciences
catalog: LS-C386654

domestic rabbit polyclonal
reactivity: human
application: western blot, ELISA, immunohistochemistry, immunocytochemistry

Western blot of Vinculin antibody
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VCL / Vinculin Antibody LS-C387510
LifeSpan Biosciences
catalog: LS-C387510

domestic rabbit polyclonal
reactivity: human
application: western blot

Western blot of Vinculin antibody
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VCL / Vinculin Antibody (HRP) LS-C550273
LifeSpan Biosciences
catalog: LS-C550273

mouse monoclonal
reactivity: human
conjugate: HRP
application: western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation

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