Cullin 2 Polyclonal Antibody | Invitrogen 51-1800 product information

product summary

company name :

Invitrogen

other brands :

NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience

product type :

antibody

product name :

Cullin 2 Polyclonal Antibody

catalog :

51-1800

quantity :

100µg

price :

US 309.00

clonality :

polyclonal

host :

rabbit

conjugate :

nonconjugated

reactivity :

human, mouse, rat

application :

western blot, ELISA, immunohistochemistry, immunoprecipitation, immunohistochemistry - paraffin section

more info or order :

Invitrogen product webpage

citations: 13

Published Application/Species/Sample/Dilution	Reference
western blot; human; 1:1000; loading ...; fig 2a	Fulcher L, MacArtney T, Bozatzi P, Hornberger A, Rojas Fernandez A, Sapkota G. An affinity-directed protein missile system for targeted proteolysis. Open Biol. 2016;6: pubmed
western blot; human; fig 6s1	Mosadeghi R, Reichermeier K, Winkler M, Schreiber A, Reitsma J, Zhang Y, et al. Structural and kinetic analysis of the COP9-Signalosome activation and the cullin-RING ubiquitin ligase deneddylation cycle. elife. 2016;5: pubmed publisher
western blot; human; 1:2500	Sandoval D, Hill S, Ziemba A, Lewis S, Kuhlman B, Kleiger G. Ubiquitin-conjugating enzyme Cdc34 and ubiquitin ligase Skp1-cullin-F-box ligase (SCF) interact through multiple conformations. J Biol Chem. 2015;290:1106-18 pubmed publisher
western blot; human; fig 2	den Besten W, Verma R, Kleiger G, Oania R, Deshaies R. NEDD8 links cullin-RING ubiquitin ligase function to the p97 pathway. Nat Struct Mol Biol. 2012;19:511-6, S1 pubmed publisher
western blot; human; fig 2	Choo Y, Boh B, Lou J, Eng J, Leck Y, Anders B, et al. Characterization of the role of COP9 signalosome in regulating cullin E3 ubiquitin ligase activity. Mol Biol Cell. 2011;22:4706-15 pubmed publisher
western blot; human; fig 1	Boh B, Smith P, Hagen T. Neddylation-induced conformational control regulates cullin RING ligase activity in vivo. J Mol Biol. 2011;409:136-45 pubmed publisher
	Heir P, Srikumar T, Bikopoulos G, Bunda S, Poon B, Lee J, et al. Oxygen-dependent Regulation of Erythropoietin Receptor Turnover and Signaling. J Biol Chem. 2016;291:7357-72 pubmed publisher
	Bett J, Ibrahim A, Garg A, Kelly V, Pedrioli P, Rocha S, et al. The P-body component USP52/PAN2 is a novel regulator of HIF1A mRNA stability. Biochem J. 2013;451:185-94 pubmed publisher
	Bandau S, Knebel A, Gage Z, Wood N, Alexandru G. UBXN7 docks on neddylated cullin complexes using its UIM motif and causes HIF1? accumulation. BMC Biol. 2012;10:36 pubmed publisher
	Mao X, Gluck N, Chen B, Starokadomskyy P, Li H, Maine G, et al. COMMD1 (copper metabolism MURR1 domain-containing protein 1) regulates Cullin RING ligases by preventing CAND1 (Cullin-associated Nedd8-dissociated protein 1) binding. J Biol Chem. 2011;286:32355-65 pubmed publisher
	Mao X, Gluck N, Li D, Maine G, Li H, Zaidi I, et al. GCN5 is a required cofactor for a ubiquitin ligase that targets NF-kappaB/RelA. Genes Dev. 2009;23:849-61 pubmed publisher
	Knauth K, Bex C, Jemth P, Buchberger A. Renal cell carcinoma risk in type 2 von Hippel-Lindau disease correlates with defects in pVHL stability and HIF-1alpha interactions. Oncogene. 2006;25:370-7 pubmed
	Lolkema M, Gervais M, Snijckers C, Hill R, Giles R, Voest E, et al. Tumor suppression by the von Hippel-Lindau protein requires phosphorylation of the acidic domain. J Biol Chem. 2005;280:22205-11 pubmed

product information

Product Type :

Antibody

Product Name :

Cullin 2 Polyclonal Antibody

Catalog # :

51-1800

Quantity :

100µg

Price :

US 309.00

Clonality :

Polyclonal

Purity :

Antigen affinity chromatography

Host :

Rabbit

Reactivity :

Human, Mouse, Rat

Applications :

ELISA: 0.1-1.0 µg/mL, Immunohistochemistry (Paraffin): 1:10-1:50, Immunoprecipitation: 2-3 µg/mL, Western Blot: 1:250-1:1000

Species :

Human, Mouse, Rat

Isotype :

IgG

Storage :

-20°C

Description :

CUL-2 is a member of the family of human cullin genes (CUL-1, -2, -3, -4a, -4b, and -5), homologous to the S. cerevisiae cdc53 gene. CUL-1 forms a complex with human p19skp1 and F box protein p45skp2. Together with cdc53 (Known as CulA), as three subunits, they form a ubiquitin ligase controlling proteolysis of G1 cell cycle regulatory proteins. In contrast to CUL-1, CUL-2 does not associate with p19skp1 and p45skp2. Instead, CUL-2 forms a complex with an inactive transcriptional elongation complex, SIII, formed by three subunits: Elongain C, Elongin B and VHL. The function of this association remains unknown.

Immunogen :

Synthetic peptide derived from the C-terminus of the human CUL2

Format :

Liquid

Applications w/Dilutions :

ELISA: 0.1-1.0 µg/mL, Immunohistochemistry (Paraffin): 1:10-1:50, Immunoprecipitation: 2-3 µg/mL, Western Blot: 1:250-1:1000

Aliases :

CUL-2

more info or order :

Invitrogen product webpage