Phospho-Tau (Ser199, Ser202) Polyclonal Antibody | Invitrogen 44-768G product information

product summary

company name :

Invitrogen

other brands :

NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience

product type :

antibody

product name :

Phospho-Tau (Ser199, Ser202) Polyclonal Antibody

catalog :

44-768G

quantity :

10 blots

price :

US 309.00

clonality :

polyclonal

host :

rabbit

conjugate :

nonconjugated

antigen modification :

phosphorylated

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section, western blot knockout validation

more info or order :

Invitrogen product webpage

citations: 16

Published Application/Species/Sample/Dilution	Reference
western blot knockout validation; human; 1:500; tbl 1 immunocytochemistry; human; 1:100; tbl 2 western blot; mouse	Ercan E, Eid S, Weber C, Kowalski A, Bichmann M, Behrendt A, et al. A validated antibody panel for the characterization of tau post-translational modifications. Mol Neurodegener. 2017;12:87 pubmed publisher
western blot; mouse; 1:1000; loading ...; fig 2A	Chen S, Sun J, Zhao G, Guo A, Chen Y, Fu R, et al. Liraglutide Improves Water Maze Learning and Memory Performance While Reduces Hyperphosphorylation of Tau and Neurofilaments in APP/PS1/Tau Triple Transgenic Mice. Neurochem Res. 2017;42:2326-2335 pubmed publisher
immunohistochemistry - paraffin section; human; 1:5000; loading ...; fig 6	Noy S, Krawitz S, Del Bigio M. Chronic Traumatic Encephalopathy-Like Abnormalities in a Routine Neuropathology Service. J Neuropathol Exp Neurol. 2016;75:1145-1154 pubmed publisher
western blot; mouse; 1:2000	Kaufman A, Salazar S, Haas L, Yang J, Kostylev M, Jeng A, et al. Fyn inhibition rescues established memory and synapse loss in Alzheimer mice. Ann Neurol. 2015;77:953-71 pubmed publisher
western blot; rat; 1:1500; fig 7	Zhang J, Tan H, Jiang W, Zuo Z. The choice of general anesthetics may not affect neuroinflammation and impairment of learning and memory after surgery in elderly rats. J Neuroimmune Pharmacol. 2015;10:179-89 pubmed publisher
western blot; mouse; fig 6	Ordóñez Gutiérrez L, Torres J, Gavin R, Anton M, Arroba Espinosa A, Espinosa J, et al. Cellular prion protein modulates ?-amyloid deposition in aged APP/PS1 transgenic mice. Neurobiol Aging. 2013;34:2793-804 pubmed publisher
western blot; rat	Lin D, Cao L, Wang Z, Li J, Washington J, Zuo Z. Lidocaine attenuates cognitive impairment after isoflurane anesthesia in old rats. Behav Brain Res. 2012;228:319-27 pubmed publisher
western blot; human	Burkhard K, Shapiro P. Use of inhibitors in the study of MAP kinases. Methods Mol Biol. 2010;661:107-22 pubmed publisher
western blot; human; 1:1000	Spatara M, Robinson A. Transgenic mouse and cell culture models demonstrate a lack of mechanistic connection between endoplasmic reticulum stress and tau dysfunction. J Neurosci Res. 2010;88:1951-61 pubmed publisher
immunohistochemistry; mouse; fig 2 western blot; mouse; fig 1	Kim B, Backus C, Oh S, Hayes J, Feldman E. Increased tau phosphorylation and cleavage in mouse models of type 1 and type 2 diabetes. Endocrinology. 2009;150:5294-301 pubmed publisher
	Mohamed N, Desjardins A, Leclerc N. Tau secretion is correlated to an increase of Golgi dynamics. PLoS ONE. 2017;12:e0178288 pubmed publisher
	Blas Rus N, Bustos Morán E, Perez de Castro I, de Carcer G, Borroto A, Camafeita E, et al. Aurora A drives early signalling and vesicle dynamics during T-cell activation. Nat Commun. 2016;7:11389 pubmed publisher
	Zhao W, Dumanis S, Tamboli I, Rodriguez G, Jo Ladu M, Moussa C, et al. Human APOE genotype affects intraneuronal A?1-42 accumulation in a lentiviral gene transfer model. Hum Mol Genet. 2014;23:1365-75 pubmed publisher
	Petratos S, Ozturk E, Azari M, Kenny R, Lee J, Magee K, et al. Limiting multiple sclerosis related axonopathy by blocking Nogo receptor and CRMP-2 phosphorylation. Brain. 2012;135:1794-818 pubmed publisher
	Zhang Q, Gao T, Luo Y, Chen X, Gao G, Gao X, et al. Transient focal cerebral ischemia/reperfusion induces early and chronic axonal changes in rats: its importance for the risk of Alzheimer's disease. PLoS ONE. 2012;7:e33722 pubmed publisher
	Zheng Y, Kesavapany S, Gravell M, Hamilton R, Schubert M, Amin N, et al. A Cdk5 inhibitory peptide reduces tau hyperphosphorylation and apoptosis in neurons. EMBO J. 2005;24:209-20 pubmed

product information

Product Type :

Antibody

Product Name :

Phospho-Tau (Ser199, Ser202) Polyclonal Antibody

Catalog # :

44-768G

Quantity :

10 blots

Price :

US 309.00

Clonality :

Polyclonal

Purity :

Antigen affinity chromatography

Host :

Rabbit

Reactivity :

Human, Mouse

Applications :

Immunocytochemistry: 1 µg/mL, Immunofluorescence: 1 µg/mL, Immunohistochemistry (Paraffin): 1:10-1:100, Western Blot: 1:1000

Species :

Human, Mouse

Isotype :

IgG

Storage :

-20°C

Description :

Tau is a neuronal microtubule-associated protein found predominantly on axons. The function of Tau is to promote tubulin polymerization and stabilize microtubules. The C-terminus binds axonal microtubules while the N- terminus binds neural plasma membrane components, suggesting that tau functions as a linker protein between both. Axonal polarity is predetermined by TAU/MAPT localization (in the neuronal cell) in the domain of the cell body defined by the centrosome. The short isoforms allow plasticity of the cytoskeleton while the longer isoforms may preferentially play a role in its stabilization. In its hyper-phosphorylated form, Tau is the major component of paired helical filaments (PHF), the building block of neurofibrillary lesions in Alzheimer's diseases (AD) brain. Hyper-phosphorylation impairs the microtubule binding function of Tau, resulting in the destabilization of microtubules in AD brains, ultimately leading to the degeneration of the affected neurons. Numerous serine/threonine kinases phosphorylate Tau, including GSK-3beta, protein kinase A (PKA), cyclindependent kinase 5 (cdk5) and casein kinase II. Hyper-phosphorylated Tau is found in neurofibrillary lesions in a range and other central nervous system disorders such as Pick's disease, frontotemporal dementia, cortico-basal degeneration and progressive supranuclear palsy.

Immunogen :

The antiserum was produced against a chemically synthesized phosphopeptide derived from the region of human tau that contains serines 199 and 202. The sequence is conserved in mouse and rat.

Format :

Liquid

Applications w/Dilutions :

Immunocytochemistry: 1 µg/mL, Immunofluorescence: 1 µg/mL, Immunohistochemistry (Paraffin): 1:10-1:100, Western Blot: 1:1000

Aliases :

MAPT, Microtubule-associated protein tau, MTBT1, Neurofibrillary tangle protein, Paired helical filament-tau, PHF-tau

more info or order :

Invitrogen product webpage