| Published Application/Species/Sample/Dilution | Reference |
|---|
- western blot; mouse; fig s2a
| Kaufman D, Papillon J, Larose L, Iwawaki T, Cybulsky A. Deletion of inositol-requiring enzyme-1α in podocytes disrupts glomerular capillary integrity and autophagy.. Mol Biol Cell. 2017;28:1636-1651 pubmed publisher |
- western blot; human; 1:1000; loading ...; fig s3i
| Villar V, Nguyen T, Delcroix V, Terés S, Bouchecareilh M, Salin B, et al. mTORC1 inhibition in cancer cells protects from glutaminolysis-mediated apoptosis during nutrient limitation. Nat Commun. 2017;8:14124 pubmed publisher |
- western blot; mouse; 1:1000; fig 3
| Zhu S, Henninger K, McGrath B, Cavener D. PERK Regulates Working Memory and Protein Synthesis-Dependent Memory Flexibility. PLoS ONE. 2016;11:e0162766 pubmed publisher |
- western blot; Caenorhabditis elegans; fig 1a
| Ferraz R, Camara H, De-Souza E, Pinto S, Pinca A, Silva R, et al. IMPACT is a GCN2 inhibitor that limits lifespan in Caenorhabditis elegans. BMC Biol. 2016;14:87 pubmed |
- western blot; mouse; fig 8
- western blot; cat; fig 1
| Humoud M, Doyle N, Royall E, Willcocks M, Sorgeloos F, van Kuppeveld F, et al. Feline Calicivirus Infection Disrupts Assembly of Cytoplasmic Stress Granules and Induces G3BP1 Cleavage. J Virol. 2016;90:6489-501 pubmed publisher |
- western blot; human; fig 8
| Nelson E, Schmidt K, Deflubé L, Doganay S, Banadyga L, Olejnik J, et al. Ebola Virus Does Not Induce Stress Granule Formation during Infection and Sequesters Stress Granule Proteins within Viral Inclusions. J Virol. 2016;90:7268-84 pubmed publisher |
- western blot; human; fig 2
| Zhong L, Wang L, Xu L, Liu Q, Jiang L, Zhi Y, et al. The cytotoxic effect of the NOS-mediated oxidative stress in MCF-7 cells after PbCl₂ exposure. Environ Toxicol. 2016;31:601-8 pubmed publisher |
- western blot; human; loading ...; fig 5
| Mohammad S, Wolfe L, Stöbe P, Biskup S, Wainwright M, Melin-Aldana H, et al. Infantile Cirrhosis, Growth Impairment, and Neurodevelopmental Anomalies Associated with Deficiency of PPP1R15B. J Pediatr. 2016;179:144-149.e2 pubmed publisher |
- western blot; rat; loading ...; fig 6a
| Ayuso M, Martínez-Alonso E, Regidor I, Alcazar A. Stress Granule Induction after Brain Ischemia Is Independent of Eukaryotic Translation Initiation Factor (eIF) 2α Phosphorylation and Is Correlated with a Decrease in eIF4B and eIF4E Proteins. J Biol Chem. 2016;291:27252-27264 pubmed publisher |
- western blot; mouse; 1:2000; loading ...; fig 1a
| Silva R, Sattlegger E, Castilho B. Perturbations in actin dynamics reconfigure protein complexes that modulate GCN2 activity and promote an eIF2 response. J Cell Sci. 2016;129:4521-4533 pubmed |
- western blot; human; 1:2000; loading ...; fig 3a
| Kainulainen M, Lau S, Samuel C, Hornung V, Weber F. NSs Virulence Factor of Rift Valley Fever Virus Engages the F-Box Proteins FBXW11 and β-TRCP1 To Degrade the Antiviral Protein Kinase PKR. J Virol. 2016;90:6140-7 pubmed publisher |
- western blot; mouse; 1:1000; loading ...; fig 1c
- western blot; human; 1:1000; loading ...; fig 5d
| Khoutorsky A, Sorge R, Prager-Khoutorsky M, Pawlowski S, Longo G, Jafarnejad S, et al. eIF2α phosphorylation controls thermal nociception. Proc Natl Acad Sci U S A. 2016;113:11949-11954 pubmed |
| Sidrauski C, McGeachy A, Ingolia N, Walter P. The small molecule ISRIB reverses the effects of eIF2α phosphorylation on translation and stress granule assembly. elife. 2015;4: pubmed publisher |
| Zhong L, Wang L, Xu L, Liu Q, Jiang L, Zhi Y, et al. The role of NOS-mediated ROS accumulation in an early phase Cu-induced acute cytotoxicity in MCF-7 cells. Biometals. 2015;28:113-22 pubmed publisher |
- western blot; human; 1:1000; fig 5
| Tholen M, Wolanski J, Stolze B, Chiabudini M, Gajda M, Bronsert P, et al. Stress-resistant Translation of Cathepsin L mRNA in Breast Cancer Progression. J Biol Chem. 2015;290:15758-69 pubmed publisher |
- western blot; baker's yeast
| Silva R, Dautel M, Di Genova B, Amberg D, Castilho B, Sattlegger E. The Gcn2 Regulator Yih1 Interacts with the Cyclin Dependent Kinase Cdc28 and Promotes Cell Cycle Progression through G2/M in Budding Yeast. PLoS ONE. 2015;10:e0131070 pubmed publisher |
- western blot; fruit fly; 1:1000
| Niehues S, Bussmann J, Steffes G, Erdmann I, Köhrer C, Sun L, et al. Impaired protein translation in Drosophila models for Charcot-Marie-Tooth neuropathy caused by mutant tRNA synthetases. Nat Commun. 2015;6:7520 pubmed publisher |
- western blot; human; 1:1000; fig s2b
| Das I, Krzyzosiak A, Schneider K, Wrabetz L, D'Antonio M, Barry N, et al. Preventing proteostasis diseases by selective inhibition of a phosphatase regulatory subunit. Science. 2015;348:239-42 pubmed publisher |
- western blot; mouse; fig 5
| Bettaieb A, Jiang J, Sasaki Y, Chao T, Kiss Z, Chen X, et al. Hepatocyte Nicotinamide Adenine Dinucleotide Phosphate Reduced Oxidase 4 Regulates Stress Signaling, Fibrosis, and Insulin Sensitivity During Development of Steatohepatitis in Mice. Gastroenterology. 2015;149:468-80.e10 pubmed publisher |
- western blot; mouse
- western blot; human
| Legrand N, Jaquier-Gubler P, Curran J. The impact of the phosphomimetic eIF2αS/D on global translation, reinitiation and the integrated stress response is attenuated in N2a cells. Nucleic Acids Res. 2015;43:8392-404 pubmed publisher |
- western blot; mouse; fig s1
- western blot; rat; fig 1
| Wang S, Park S, Kodali V, Han J, Yip T, Chen Z, et al. Identification of protein disulfide isomerase 1 as a key isomerase for disulfide bond formation in apolipoprotein B100. Mol Biol Cell. 2015;26:594-604 pubmed publisher |
- western blot; mouse; 1:1000; fig 3
| Hengstermann A, Muller T. Endoplasmic reticulum stress induced by aqueous extracts of cigarette smoke in 3T3 cells activates the unfolded-protein-response-dependent PERK pathway of cell survival. Free Radic Biol Med. 2008;44:1097-107 pubmed publisher |
- western blot; human; fig 5
| Drummond M, Fry C, Glynn E, Timmerman K, Dickinson J, Walker D, et al. Skeletal muscle amino acid transporter expression is increased in young and older adults following resistance exercise. J Appl Physiol (1985). 2011;111:135-42 pubmed publisher |
| Garcia M, Carrasco E, Aguilera M, Alvarez P, Rivas C, Campos J, et al. The chemotherapeutic drug 5-fluorouracil promotes PKR-mediated apoptosis in a p53-independent manner in colon and breast cancer cells. PLoS ONE. 2011;6:e23887 pubmed publisher |
| Yuan C, Sharma N, Gilge D, Stanley W, Li Y, Hatzoglou M, et al. Preserved protein synthesis in the heart in response to acute fasting and chronic food restriction despite reductions in liver and skeletal muscle. Am J Physiol Endocrinol Metab. 2008;295:E216-22 pubmed publisher |
- western blot; fission yeast; 1:3000
| Rødland G, Tvegård T, Boye E, Grallert B. Crosstalk between the Tor and Gcn2 pathways in response to different stresses. Cell Cycle. 2014;13:453-61 pubmed publisher |
- western blot; mouse; fig 2
| Chikka M, McCabe D, Tyra H, Rutkowski D. C/EBP homologous protein (CHOP) contributes to suppression of metabolic genes during endoplasmic reticulum stress in the liver. J Biol Chem. 2013;288:4405-15 pubmed publisher |
- western blot; budding yeasts; fig 2
| Tarumoto Y, Kanoh J, Ishikawa F. Receptor for activated C-kinase (RACK1) homolog Cpc2 facilitates the general amino acid control response through Gcn2 kinase in fission yeast. J Biol Chem. 2013;288:19260-8 pubmed publisher |
- immunocytochemistry; mouse; fig 1
| Palam L, Baird T, Wek R. Phosphorylation of eIF2 facilitates ribosomal bypass of an inhibitory upstream ORF to enhance CHOP translation. J Biol Chem. 2011;286:10939-49 pubmed publisher |
- western blot; human; 1:1000; fig 4
| Burnett H, Audas T, Liang G, Lu R. Herpes simplex virus-1 disarms the unfolded protein response in the early stages of infection. Cell Stress Chaperones. 2012;17:473-83 pubmed publisher |
- western blot; mouse; fig 2
| Zhou D, Palam L, Jiang L, Narasimhan J, Staschke K, Wek R. Phosphorylation of eIF2 directs ATF5 translational control in response to diverse stress conditions. J Biol Chem. 2008;283:7064-73 pubmed publisher |
| Legrand N, Araud T, Conne B, Kuijpers O, Jaquier-Gubler P, Curran J. An AUG codon conserved for protein function rather than translational initiation: the story of the protein sElk1. PLoS ONE. 2014;9:e102890 pubmed publisher |
- immunohistochemistry - free floating section; mouse; fig 1
- western blot; mouse; fig 5
| Ma T, Trinh M, Wexler A, Bourbon C, Gatti E, Pierre P, et al. Suppression of eIF2α kinases alleviates Alzheimer's disease-related plasticity and memory deficits. Nat Neurosci. 2013;16:1299-305 pubmed publisher |
- western blot; human; fig 3
| Galicia-Vázquez G, Cencic R, Robert F, Agenor A, Pelletier J. A cellular response linking eIF4AI activity to eIF4AII transcription. RNA. 2012;18:1373-84 pubmed publisher |
- western blot; mouse; fig 5
| Arensdorf A, Rutkowski D. Endoplasmic reticulum stress impairs IL-4/IL-13 signaling through C/EBPβ-mediated transcriptional suppression. J Cell Sci. 2013;126:4026-36 pubmed publisher |
- western blot; human; fig 3
| Zhong L, Wang L, Xu L, Liu Q, Jiang L, Zhi Y, et al. The role of nitric oxide synthase signaling pathway in the Zn-induced cellular responses in MCF-7 cells. Environ Toxicol Pharmacol. 2014;38:783-91 pubmed publisher |
- western blot; human; fig 10
| Shabman R, Hoenen T, Groseth A, Jabado O, Binning J, Amarasinghe G, et al. An upstream open reading frame modulates ebola virus polymerase translation and virus replication. PLoS Pathog. 2013;9:e1003147 pubmed publisher |
- western blot; rat; 1:3000; fig 2
| Tuckow A, Vary T, Kimball S, Jefferson L. Ectopic expression of eIF2Bepsilon in rat skeletal muscle rescues the sepsis-induced reduction in guanine nucleotide exchange activity and protein synthesis. Am J Physiol Endocrinol Metab. 2010;299:E241-8 pubmed publisher |
- western blot; mouse; fig 6
| Ventoso I, Berlanga J, Almendral J. Translation control by protein kinase R restricts minute virus of mice infection: role in parvovirus oncolysis. J Virol. 2010;84:5043-51 pubmed publisher |
- western blot; mouse; fig 1
| Yamani L, Latreille M, Larose L. Interaction of Nck1 and PERK phosphorylated at Y⁵⁶¹ negatively modulates PERK activity and PERK regulation of pancreatic β-cell proinsulin content. Mol Biol Cell. 2014;25:702-11 pubmed publisher |
- western blot; African green monkey
| Cassady K. Human cytomegalovirus TRS1 and IRS1 gene products block the double-stranded-RNA-activated host protein shutoff response induced by herpes simplex virus type 1 infection. J Virol. 2005;79:8707-15 pubmed |
- western blot; mouse; fig 3
| Harding H, Lackey J, Hsu H, Zhang Y, Deng J, Xu R, et al. An intact unfolded protein response in Trpt1 knockout mice reveals phylogenic divergence in pathways for RNA ligation. RNA. 2008;14:225-32 pubmed |
- western blot; mouse; fig 1
| Jiang H, Jiang L, Wek R. The eukaryotic initiation factor-2 kinase pathway facilitates differential GADD45a expression in response to environmental stress. J Biol Chem. 2007;282:3755-65 pubmed |
- western blot; human; 1:500; fig 3
| Myers J, Chi J, Gong D, Schaner M, Brown P, Ferrell J. Minimizing off-target effects by using diced siRNAs for RNA interference. J RNAi Gene Silencing. 2006;2:181-94 pubmed |
- western blot; rat; 1:750; fig 2
| Roberts G, Di Loreto M, Marshall M, Wang J, DeGracia D. Hippocampal cellular stress responses after global brain ischemia and reperfusion. Antioxid Redox Signal. 2007;9:2265-75 pubmed |
| Shah A, Parker J, Gillespie G, Lakeman F, Meleth S, Markert J, et al. Enhanced antiglioma activity of chimeric HCMV/HSV-1 oncolytic viruses. Gene Ther. 2007;14:1045-54 pubmed |
| Tong L, Wu S. The role of constitutive nitric-oxide synthase in ultraviolet B light-induced nuclear factor κB activity. J Biol Chem. 2014;289:26658-68 pubmed publisher |
| Li Y, Bevilacqua E, Chiribau C, Majumder M, Wang C, Croniger C, et al. Differential control of the CCAAT/enhancer-binding protein beta (C/EBPbeta) products liver-enriched transcriptional activating protein (LAP) and liver-enriched transcriptional inhibitory protein (LIP) and the regulation of gene expression during the respo. J Biol Chem. 2008;283:22443-56 pubmed publisher |
- western blot; human; fig 7
| Shirai A, Sadaie M, Shinmyozu K, Nakayama J. Methylation of ribosomal protein L42 regulates ribosomal function and stress-adapted cell growth. J Biol Chem. 2010;285:22448-60 pubmed publisher |
- western blot; human; fig 2
| Rodriguez M, Domingo E, Alonso S, Frade J, Eiros J, Crespo M, et al. The unfolded protein response and the phosphorylations of activating transcription factor 2 in the trans-activation of il23a promoter produced by β-glucans. J Biol Chem. 2014;289:22942-57 pubmed publisher |
- western blot; mouse; 1:1000; fig 6
| Miyamoto Y, Mauer A, Kumar S, Mott J, Malhi H. Mmu-miR-615-3p regulates lipoapoptosis by inhibiting C/EBP homologous protein. PLoS ONE. 2014;9:e109637 pubmed publisher |
- western blot; human; fig 10
| Yu Y, Pierciey F, Maguire T, Alwine J. PKR-like endoplasmic reticulum kinase is necessary for lipogenic activation during HCMV infection. PLoS Pathog. 2013;9:e1003266 pubmed publisher |
- western blot; human; 1:1000
| Spatara M, Robinson A. Transgenic mouse and cell culture models demonstrate a lack of mechanistic connection between endoplasmic reticulum stress and tau dysfunction. J Neurosci Res. 2010;88:1951-61 pubmed publisher |
| Liao M, Pabarcus M, Wang Y, Hefner C, Maltby D, Medzihradszky K, et al. Impaired dexamethasone-mediated induction of tryptophan 2,3-dioxygenase in heme-deficient rat hepatocytes: translational control by a hepatic eIF2alpha kinase, the heme-regulated inhibitor. J Pharmacol Exp Ther. 2007;323:979-89 pubmed |
| Eng K, Panas M, Murphy D, Karlsson Hedestam G, McInerney G. Accumulation of autophagosomes in Semliki Forest virus-infected cells is dependent on expression of the viral glycoproteins. J Virol. 2012;86:5674-85 pubmed publisher |
| Oyadomari S, Harding H, Zhang Y, Oyadomari M, Ron D. Dephosphorylation of translation initiation factor 2alpha enhances glucose tolerance and attenuates hepatosteatosis in mice. Cell Metab. 2008;7:520-32 pubmed publisher |
| Lu G, Ota A, Ren S, Franklin S, Rau C, Ping P, et al. PPM1l encodes an inositol requiring-protein 1 (IRE1) specific phosphatase that regulates the functional outcome of the ER stress response. Mol Metab. 2013;2:405-16 pubmed publisher |
| Perzlmaier A, Richter F, Seufert W. Translation initiation requires cell division cycle 123 (Cdc123) to facilitate biogenesis of the eukaryotic initiation factor 2 (eIF2). J Biol Chem. 2013;288:21537-46 pubmed publisher |
| Herzog B, Popova B, Jakobshagen A, Shahpasandzadeh H, Braus G. Mutual cross talk between the regulators Hac1 of the unfolded protein response and Gcn4 of the general amino acid control of Saccharomyces cerevisiae. Eukaryot Cell. 2013;12:1142-54 pubmed publisher |
| Brush M, Weiser D, Shenolikar S. Growth arrest and DNA damage-inducible protein GADD34 targets protein phosphatase 1 alpha to the endoplasmic reticulum and promotes dephosphorylation of the alpha subunit of eukaryotic translation initiation factor 2. Mol Cell Biol. 2003;23:1292-303 pubmed |
| Muaddi H, Majumder M, Peidis P, Papadakis A, Holcik M, Scheuner D, et al. Phosphorylation of eIF2α at serine 51 is an important determinant of cell survival and adaptation to glucose deficiency. Mol Biol Cell. 2010;21:3220-31 pubmed publisher |
| Nejepinska J, Malik R, Wagner S, Svoboda P. Reporters transiently transfected into mammalian cells are highly sensitive to translational repression induced by dsRNA expression. PLoS ONE. 2014;9:e87517 pubmed publisher |
| Busnadiego I, Maestre A, Rodriguez D, Rodriguez J. The infectious bursal disease virus RNA-binding VP3 polypeptide inhibits PKR-mediated apoptosis. PLoS ONE. 2012;7:e46768 pubmed publisher |
| Qiu Y, Mao T, Zhang Y, Shao M, You J, Ding Q, et al. A crucial role for RACK1 in the regulation of glucose-stimulated IRE1alpha activation in pancreatic beta cells. Sci Signal. 2010;3:ra7 pubmed publisher |
| von Holzen U, Pataer A, Raju U, Bocangel D, Vorburger S, Liu Y, et al. The double-stranded RNA-activated protein kinase mediates radiation resistance in mouse embryo fibroblasts through nuclear factor kappaB and Akt activation. Clin Cancer Res. 2007;13:6032-9 pubmed |
| Zhang P, McGrath B, Li S, Frank A, Zambito F, Reinert J, et al. The PERK eukaryotic initiation factor 2 alpha kinase is required for the development of the skeletal system, postnatal growth, and the function and viability of the pancreas. Mol Cell Biol. 2002;22:3864-74 pubmed |
| Dey M, Cao C, Dar A, Tamura T, Ozato K, Sicheri F, et al. Mechanistic link between PKR dimerization, autophosphorylation, and eIF2alpha substrate recognition. Cell. 2005;122:901-13 pubmed |
| Qian Z, Xuan B, Chapa T, Gualberto N, Yu D. Murine cytomegalovirus targets transcription factor ATF4 to exploit the unfolded-protein response. J Virol. 2012;86:6712-23 pubmed publisher |
| Lu W, Wu S. Mechanism for dynamic regulation of iNOS expression after UVB-irradiation. Mol Carcinog. 2013;52:627-33 pubmed publisher |
| Bøe C, Krohn M, Rødland G, Capiaghi C, Maillard O, Thoma F, et al. Induction of a G1-S checkpoint in fission yeast. Proc Natl Acad Sci U S A. 2012;109:9911-6 pubmed publisher |
