TRA-1-81 Monoclonal Antibody (cl.26) | Invitrogen 41-1100 product information

product summary

company name :

Invitrogen

other brands :

NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience

product type :

antibody

product name :

TRA-1-81 Monoclonal Antibody (cl.26)

catalog :

41-1100

quantity :

100µg

price :

US 309.00

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

cl.26

reactivity :

human

application :

western blot, ELISA, immunocytochemistry, immunoprecipitation, flow cytometry

more info or order :

Invitrogen product webpage

citations: 9

Published Application/Species/Sample/Dilution	Reference
immunocytochemistry; human; fig 1	Reboun M, Rybová J, Dobrovolny R, Vcelak J, Veselková T, Storkanova G, et al. X-Chromosome Inactivation Analysis in Different Cell Types and Induced Pluripotent Stem Cells Elucidates the Disease Mechanism in a Rare Case of Mucopolysaccharidosis Type II in a Female. Folia Biol (Praha). 2016;62:82-9 pubmed
immunocytochemistry; human; 1:100; fig 1	Alves C, Dariolli R, Jorge F, Monteiro M, Maximino J, Martins R, et al. Gene expression profiling for human iPS-derived motor neurons from sporadic ALS patients reveals a strong association between mitochondrial functions and neurodegeneration. Front Cell Neurosci. 2015;9:289 pubmed publisher
western blot; human; 1:100	Wong A, Chin S, Xia S, Garner J, Bear C, Rossant J. Efficient generation of functional CFTR-expressing airway epithelial cells from human pluripotent stem cells. Nat Protoc. 2015;10:363-81 pubmed publisher
immunocytochemistry; human	Afzal M, Strande J. Generation of induced pluripotent stem cells from muscular dystrophy patients: efficient integration-free reprogramming of urine derived cells. J Vis Exp. 2015;:52032 pubmed publisher
immunocytochemistry; human; fig 8	Ruff D, Macarthur C, Tran H, Bergseid J, Tian J, Shannon M, et al. Applications of quantitative polymerase chain reaction protein assays during reprogramming. Stem Cells Dev. 2012;21:530-8 pubmed publisher
flow cytometry; human	Hotta A, Cheung A, Farra N, Vijayaragavan K, Séguin C, Draper J, et al. Isolation of human iPS cells using EOS lentiviral vectors to select for pluripotency. Nat Methods. 2009;6:370-6 pubmed publisher
	Quintanilla R. Cellular characterization of human pluripotent stem cells. Methods Mol Biol. 2013;997:179-90 pubmed publisher
	MacArthur C, Fontes A, Ravinder N, Kuninger D, Kaur J, Bailey M, et al. Generation of human-induced pluripotent stem cells by a nonintegrating RNA Sendai virus vector in feeder-free or xeno-free conditions. Stem Cells Int. 2012;2012:564612 pubmed publisher
	Andrews P, Casper J, Damjanov I, Duggan Keen M, Giwercman A, Hata J, et al. Comparative analysis of cell surface antigens expressed by cell lines derived from human germ cell tumours. Int J Cancer. 1996;66:806-16 pubmed

product information

Product Type :

Antibody

Product Name :

TRA-1-81 Monoclonal Antibody (cl.26)

Catalog # :

41-1100

Quantity :

100µg

Price :

US 309.00

Clonality :

Monoclonal

Purity :

protein A

Host :

Mouse

Reactivity :

Human

Applications :

ELISA: Assay Dependent, Immunocytochemistry: 2 µg/mL, Immunofluorescence: 5-10 µg/mL, Immunoprecipitation: 2-3 µg/mL, Western Blot: 1-5 µg/mL

Species :

Human

Clone :

cl.26

Isotype :

IgM

Storage :

-20°C

Description :

TRA-1-60 is a cell surface antigen, expressed along with SSEA-3, SSEA-4, and TRA-1-81 in human embryonic stem cells, embryonal carcinoma cells (EC), and induced pluripotent stem cells (iPS). These surface markers are lost during the differentiation process. In contrast, SSEA-1 is absent in undifferentiated human stem cells but is present on the cell surface after retinoic acid-mediated differentiation. The TRA-1-81 antigen is associated with a polydisperse glycoprotein with a molecular weight in excess of 200,000.

Immunogen :

2102Ep cl.2A6 human embryonal carcinoma cells

Format :

Liquid

Applications w/Dilutions :

ELISA: Assay Dependent, Immunocytochemistry: 2 µg/mL, Immunofluorescence: 5-10 µg/mL, Immunoprecipitation: 2-3 µg/mL, Western Blot: 1-5 µg/mL

Aliases :

Keratan sulfate antigen TRA1-81; TRA180

more info or order :

Invitrogen product webpage